Anticancer, antimicrobial, and analgesic activities of spider venoms

Akef, Hassan M.

doi:10.1039/c8tx00022k

Cited by 23 publications

(15 citation statements)

References 114 publications

(173 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As in previous cases, spider venoms are a complex mixture being of interest in oncotherapy by the peptides they content such as Brachynin, Lycosin-I, and Gomesin presented in the species Brachypelma albopilosum, Lycosa carolinensis and Acanthoscurria gomesiana respectively [91]. Hymenoptera venoms, those which come from bees (Apis mellifera) and wasps (Polybia paulista) contain Melittin and Mastoparan as the main important compounds respectively, because of their evidenced anticancer properties as it has been reviewed in some works [94,95].…”

Section: Zootherapymentioning

confidence: 85%

See 1 more Smart Citation

Zoopharmacology: A Way to Discover New Cancer Treatments

et al. 2020

View full text Add to dashboard Cite

Zoopharmacognosy is the multidisciplinary approach of the self-medication behavior of many kinds of animals. Recent studies showed the presence of antitumoral secondary metabolites in some of the plants employed by animals and their use for the same therapeutic purposes in humans. Other related and sometimes confused term is Zootherapy, which consists on the employment of animal parts and/or their by-products such as toxins, venoms, etc., to treat different human ailments. Therefore, the aim of this work is to provide a brief insight for the use of Zoopharmacology (comprising Zoopharmacognosy and Zootherapy) as new paths to discover drugs studying animal behavior and/or using compounds derived from animals. This work is focused on the approaches related to cancer, in order to propose a new promising line of research to overcome multidrug resistance (MDR). This novel subject will encourage the use of new alternative prospective ways to find new medicines.

show abstract

Section: Zootherapymentioning

confidence: 85%

Section: Zootherapymentioning

confidence: 99%

Zoopharmacology: A Way to Discover New Cancer Treatments

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Compounds produced by different organisms found in nature represent a valuable alternative for the discovery of new agents with therapeutic potential [ 1 - 3 ]. The search for agents with antimicrobial action is especially important, given the establishment of bacterial strains resistant to conventional drugs [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity and partial chemical structure of acylpolyamines isolated from the venom of the spider Acanthoscurria natalensis

Barth

Silva

Santos

et al. 2022

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Background: Acylpolyamines are one of the main non-peptide compounds present in spider venom and represent a promising alternative in the search for new molecules with antimicrobial action. Methods: The venom of Acanthoscurria natalensis spider was fractionated by reverse-phase liquid chromatography (RP-HPLC) and the antimicrobial activity of the fractions was tested using a liquid growth inhibition assay. The main antimicrobial fraction containing acylpolyamines (ApAn) was submitted to two additional chromatographic steps and analyzed by MALDI-TOF. Fractions of interest were accumulated for ultraviolet (UV) spectroscopy and ESI-MS/MS analysis and for minimum inhibitory concentration (MIC) and hemolytic activity determination. Results: Five acylpolyamines were isolated from the venom with molecular masses between 614 Da and 756 Da, being named ApAn728, ApAn614a, ApAn614b, ApAn742 and ApAn756. The analysis of UV absorption profile of each ApAn and the fragmentation pattern obtained by ESI-MS/MS suggested the presence of a tyrosyl unit as chromophore and a terminal polyamine chain consistent with structural units PA43 or PA53. ApAn presented MIC between 128 µM and 256 µM against Escherichia coli and Staphylococcus aureus , without causing hemolysis against mouse erythrocytes. Conclusion: The antimicrobial and non-hemolytic properties of the analyzed ApAn may be relevant for their application as possible therapeutic agents and the identification of an unconventional chromophore for spider acylpolyamines suggests an even greater chemical diversity.

show abstract

“…Anticancer peptides (ACPs) and their derivatives have a broad anticancer spectrum and strong and rapid anticancer effects, which means they can be considered good candidate precursor molecules for cancer therapy. Natural toxins from venomous animals such as snakes, spiders, bees, scorpions, and conus, are precious resources for developing novel anticancer drugs [2][3][4][5][6][7][8]. For instance, lycosin-I, isolated from the spider Lycosa singoriensis, with a linear amphipathic alpha-helical conformation, can effectively suppress tumor growth in vitro and in vivo [9].…”

Section: Introductionmentioning

confidence: 99%

Fatty Acid Modification of the Anticancer Peptide LVTX-9 to Enhance Its Cytotoxicity against Malignant Melanoma Cells

Chen

et al. 2021

Toxins

View full text Add to dashboard Cite

Spider venom is a valuable resource for the development of novel anticancer drugs. In this study, we focused on novel linear amphipathic α-helical anticancer peptide LVTX-9, which was derived from the cDNA library of the venom gland of the spider Lycosa vittata. The cytotoxicity of LVTX-9 against murine melanoma cells in the range of 1.56–200 μM was tested and found to be significantly lower than those of most anticancer peptides reported. Its IC50 was determined to be 59.2 ± 19.8 μM in a serum or 76.3 ± 12.7 μM in serum-free medium. Fatty acid modification is a promising strategy for improving peptide performance. Therefore, to enhance the cytotoxic activity of LVTX-9, fatty acid modification of this peptide was performed, and five different carbon chain length lipopeptides named LVTX-9-C12-C20 were produced. Among them, the lipopeptide LVTX-9-C18 showed the highest cytotoxic activity in relation to B16-F10 cells, whether in a serum or serum-free medium. Most importantly, the cytotoxic activity of LVTX-9-C18 was improved by about 12.9 times in a serum medium or 19.3 times in a serum-free medium compared to that of LVTX-9. Subsequently, assays including scanning electron microscopy, trypan blue staining, lactate dehydrogenase leakage assay, and hemolytic activity could indicate that the potential direct cell membrane disruption is the main mechanism of LVTX-9-C18 to induce cancer cell death. Furthermore, the LVTX-9-C18 also showed strong cytotoxicity in relation to 3D B16-F10 spheroids, which indicates it might be a promising lead for developing anticancer drugs.

show abstract

Anticancer, antimicrobial, and analgesic activities of spider venoms

Cited by 23 publications

References 114 publications

Zoopharmacology: A Way to Discover New Cancer Treatments

Zoopharmacology: A Way to Discover New Cancer Treatments

Antimicrobial activity and partial chemical structure of acylpolyamines isolated from the venom of the spider Acanthoscurria natalensis

Fatty Acid Modification of the Anticancer Peptide LVTX-9 to Enhance Its Cytotoxicity against Malignant Melanoma Cells

Contact Info

Product

Resources

About