Sphingomyelinase cleavage of sphingomyelin in pure and mixed lipid membranes. Influence of the physical state of the sphingolipid

Ruiz-Argüello, M. Begoña; Veiga, M. P.; Arrondo, José Luis R.; Goñi, Félix M.; Alonso, Alicia

doi:10.1016/s0009-3084(01)00195-5

Cited by 59 publications

(67 citation statements)

References 36 publications

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“…Interestingly, whereas increasing proportions of an unsaturated PC added to a model membrane containing a saturated SM such as 16:0 SM progressively decreased the Tt of the latter ( 32 ), in the present study, the addition of increasing proportions of highly unsaturated SMs decreased the Tt of a saturated glycerophospholipid such as DMPC ( Table 3 ). Thus, in this case, n-V and h-V SMs played the role of the "fl uidizing" lipid of the system, contrary to the general consensus that SMs have a lipid mobility-restraining effect in bilayers.…”

Section: N-v Sm and H-v Sm Interactions With Lipidscontrasting

confidence: 41%

Unique thermal behavior of sphingomyelin species with nonhydroxy and 2-hydroxy very-long-chain (C28-C32) PUFAs

Peñalva

Furland

López

et al. 2013

Journal of Lipid Research

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Sphingomyelin (SM) is a ubiquitous sphingolipid component of cell membranes in vertebrate tissues. Like phosphatidylcholine (PC), it has phosphorylcholine as the head group, its hydrophobic moieties being a long-chain or a very-long-chain fatty acid amide-bound to the long-chain base sphingosine. Molecular species naturally occurring in mammalian SM often contain saturated (16:0, 18:0, 24:0) or monounsaturated (18:1, 22:1, 24:1) fatty acids ( 1 ).The testis and spermatozoa of various mammals including man contain SM species with an infrequent series of very-long-chain polyunsaturated fatty acids (VLCPUFAs) ( 2 ). These are elongated versions of ordinary 20-or 22-carbon atom tetraenoic, pentaenoic, and hexaenoic fatty acids of the n-3 or the n-6 series, according to the mammalian species. Whereas SMs from bovine and ovine spermatozoa are exceedingly rich in n-3 VLCPUFA with up to 34 carbon atoms, those from rodent germ and sperm cells mostly contain tetraenoic and pentaenoic fatty acids of the n-6 series with 28 to 32 carbon atoms, representative components being 28:4n-6, 30:5n-6, and 32:5n-6 ( 2, 3 ).In some mammals including the rat, a unique series of molecular species of SM containing 2-hydroxylated derivatives of the mentioned VLCPUFA also occurs in testis and spermatozoa ( 4 ). In adult rat testis, the SM species having nonhydroxy VLCPUFA and 2-hydroxy VLCPUFA (n-V SM and h-V SM, respectively) are exclusive components of spermatogenic cells, as are the corresponding ceramides containing Abstract In rat germ cells and spermatozoa, sphingomyelin (SM) contains molecular species with nonhydroxy (n) and 2-hydroxy (h) very-long-chain polyunsaturated fatty acids (V), the most abundant being SMs with (n-and h-) 28:4n-6, 30:5n-6, and 32:5n-6 as acyl chains. The aim of this study was to gain information about their thermotropic behavior and interactions with other lipids. After isolation from rat testis, multilamellar and giant unilamellar vesicles from these SMs were examined using fl uorescent probes.

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Section: N-v Sm and H-v Sm Interactions With Lipidscontrasting

confidence: 41%

Unique thermal behavior of sphingomyelin species with nonhydroxy and 2-hydroxy very-long-chain (C28-C32) PUFAs

Peñalva

Furland

López

et al. 2013

Journal of Lipid Research

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“…Catalytic activity is affected by the phase of the lipid, with gel phase SM vesicles showing relatively low activity. By contrast, mixed vesicles (SM plus PCs, phosphatidylethanolamines or Chol) or SM vesicles above the lipid melting temperature exhibit significantly higher activity [52,53].…”

Section: Enzymatic Ceramide Generation In Supported Membranesmentioning

confidence: 92%

Ceramide-enriched microdomains in planar membranes

Zou

Johnston

2010

Current Opinion in Colloid & Interface Science

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Section: Bi-directional Information Transduction Between Surface Domamentioning

confidence: 99%

“…Lipid mixing-demixing processes and concomitant structuring of segregated domains with different lipid composition or phase state profoundly influence the pre-catalytic and catalytic steps of the enzymatic reactions (10,41,45,49). A common structural element in all of these factors is the existence of lateral and/or transverse packing defects and interface tensions introduced by changes of lipid composition, anisotropic interactions, phase coexistence and connectivity of the domain lattice (10,21,41,45,49). Within this context, critical amounts of non-substrate lipids or proteins that may induce or disrupt specific surface super-structuring (10,45,47) over ranges that exceed the local intermolecular interactions become important membraneregulating factors, and some of the topological variations may be induced by the phosphohydrolytic enzymes themselves.…”

Section: Bi-directional Information Transduction Between Surface Domamentioning

confidence: 99%

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Protein-mediated surface structuring in biomembranes

Maggio

Rosetti

Borioli

et al. 2005

Braz J Med Biol Res

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The lipids and proteins of biomembranes exhibit highly dissimilar conformations, geometrical shapes, amphipathicity, and thermodynamic properties which constrain their two-dimensional molecular packing, electrostatics, and interaction preferences. This causes inevitable development of large local tensions that frequently relax into phase or compositional immiscibility along lateral and transverse planes of the membrane. On the other hand, these effects constitute the very codes that mediate molecular and structural changes determining and controlling the possibilities for enzymatic activity, apposition and recombination in biomembranes. The presence of proteins constitutes a major perturbing factor for the membrane sculpturing both in terms of its surface topography and dynamics. We will focus on some results from our group within this context and summarize some recent evidence for the active involvement of extrinsic (myelin basic protein), integral (Folch-Lees proteolipid protein) and amphitropic (cFos and c-Jun) proteins, as well as a membrane-active amphitropic phosphohydrolytic enzyme (neutral sphingomyelinase), in the process of lateral segregation and dynamics of phase domains, sculpturing of the surface topography, and the bi-directional modulation of the membrane biochemical reactivity.

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Sphingomyelinase cleavage of sphingomyelin in pure and mixed lipid membranes. Influence of the physical state of the sphingolipid

Cited by 59 publications

References 36 publications

Unique thermal behavior of sphingomyelin species with nonhydroxy and 2-hydroxy very-long-chain (C28-C32) PUFAs

Unique thermal behavior of sphingomyelin species with nonhydroxy and 2-hydroxy very-long-chain (C28-C32) PUFAs

Ceramide-enriched microdomains in planar membranes

Protein-mediated surface structuring in biomembranes

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