Sphingomyelin is critical in organizing phosphoinositide dynamics during phagocytic uptake of <i>Mycobacterium tuberculosis</i>

Niekamp, Patrick; Guzman, Gaelen; Leier, Hans C.; Rashidfarrokhi, Ali; Richina, Veronica; Pott, Fabian; Holthuis, Joost C. M.; Tafesse, Fikadu G.

doi:10.1101/565226

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…Results observed by Driss et al (2012), suggest that LAM has an eosinophil anti-inflammatory effect, as LAM down-regulate the release of peroxidase and TNF through the CD11b/CD18-LAM interaction, which limits the TLR-mediated activation (Figure 3C). In this regard, Niekamp et al (2019) have provided evidence in support of Mtb-catabolic activity induction in the sphingolipid pathway, consequently remodeling lipid rafts during phagocytosis. Similarly, it has been suggested that LAM modulates the ceramide synthesis and alters the ERK-dependent signaling pathway (Sirkar and Majumdar, 2002).…”

Section: Modulation Of the Innate Immunity By Lammentioning

confidence: 86%

Lipoarabinomannan as a Point-of-Care Assay for Diagnosis of Tuberculosis: How Far Are We to Use It?

2021

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Tuberculosis (TB) is still a severe public health problem; the current diagnostic tests have limitations that delay treatment onset. Lipoarabinomannan (LAM) is a glycolipid that is a component of the cell wall of the bacillus Mycobacterium tuberculosis, the etiologic agent of TB. This glycolipid is excreted as a soluble form in urine. The World Health Organization has established that the design of new TB diagnostic methods is one of the priorities within the EndTB Strategy. LAM has been suggested as a biomarker to develop diagnostic tests based on its identification in urine, and it is one of the most prominent candidates to develop point-of-care diagnostic test because urine samples can be easily collected. Moreover, LAM can regulate the immune response in the host and can be found in the serum of TB patients, where it probably affects a wide variety of host cell populations, consequently influencing the quality of both innate and adaptive immune responses during TB infection. Here, we revised the evidence that supports that LAM could be used as a tool for the development of new point-of-care tests for TB diagnosis, and we discussed the mechanisms that could contribute to the low sensitivity of diagnostic testing.

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Section: Modulation Of the Innate Immunity By Lammentioning

confidence: 86%

Lipoarabinomannan as a Point-of-Care Assay for Diagnosis of Tuberculosis: How Far Are We to Use It?

2021

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“…Knock-out of the gene encoding sphingomyelin synthase (SMase) 1 in human monocytic leukemia cell line U937 cells was found to inhibit the phagocytosis of M. tuberculosis. Moreover, knockout of the gene encoding serine palmitoyltransferase 2 impaired clustering of PRRs, such as Dectin-1, in RAW264.7 cells (Niekamp et al, 2019). The phosphatase CD45 did remained at the receptorpathogen contact site of nascent phagosomes.…”

Section: Role Of Sphingolipids and Their Metabolites On Antigen Prese...mentioning

confidence: 97%

Glycolipids: Linchpins in the Organization and Function of Membrane Microdomains

Hanafusa

Hotta

Iwabuchi

2020

Front. Cell Dev. Biol.

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Membrane microdomains, also called lipid rafts, are areas on membrane enriched in glycolipids, sphingolipids, and cholesterol. Although membrane microdomains are thought to play key roles in many cellular functions, their structures, properties, and biological functions remain obscure. Cellular membranes contain several types of glycoproteins, glycolipids, and other lipids, including cholesterol, glycerophospholipids, and sphingomyelin. Depending on their physicochemical properties, especially the characteristics of their glycolipids, various microdomains form on these cell membranes, providing structural or functional contextures thought to be essential for biological activities. For example, the plasma membranes of human neutrophils are enriched in lactosylceramide (LacCer) and phosphatidylglucoside (PtdGlc), each of which forms different membrane microdomains with different surrounding molecules and is involved in different functions of neutrophils. Specifically, LacCer forms Lyn-coupled lipid microdomains, which mediate neutrophil chemotaxis, phagocytosis, and superoxide generation, whereas PtdGlc-enriched microdomains mediate neutrophil differentiation and spontaneous apoptosis. However, the mechanisms by which these glycolipids form different nano/meso microdomains and mediate their specialized functions remain incompletely understood. This review describes current understanding of the roles of glycolipids and sphingolipids in their enriched contextures on cellular membranes, including their mechanisms of facilitation and regulation of intracellular signaling. This review also introduces new concepts about the roles of glycolipid and sphingolipiddependent contextures in immunological functions.

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“…[108] RD has been used for HPTLC-lipid detection in bioresearch, often as unique detection technique, without SD. [109][110][111][112][113][114][115][116][117] Due to considerations of safety, need of skills in nuclear methodology, and equipment cost, its implementation has been restricted to the laboratories working in nuclear techniques.…”

Section: Sd and Radio-densitometry (Rd)mentioning

confidence: 99%

Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

Cebolla

Jarne

Vela

et al. 2021

Journal of Liquid Chromatography & Related Technologies

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This work is a review on HPTLC contribuon to lipid analysis in complex matrices, in the period from 2010 to now, lapse of me where hyphenaon with other techniques, especially Mass Spectrometry, has experienced an important growth.Scanning densitometry (SD)-UV/FL of separated lipids, as the core of detecon and centerpiece for hyphenaon, and its coupling with MS using so ionizaon techniques (ESI, APCI, MALDI, DESI and others), are the central axis of this work. The occasional intercalaon in this coupling of an on-plate biological assay for eect-direct analysis (EDA) of lipids, as well as the combinaon of SD with radiodensitometry (RD) using isotopically labeled-lipids are also covered topics.The described techniques make possible to develop strategies for obtaining qualitave and dierent levels of quantave informaon, including untargeted lipids species.HPTLC of lipids has been used for: comparave purposes; ngerprinng; semi-quantave determinaon, idencaon of species; or quantave determinaon of a given individual lipid. Goals in molecular biology and biochemistry-related samples have been: preparave isolaons; control of purity; vericaon of metabolic products; on-plate biological assays; proles in cells; analysis of products from the cellular metabolism; measurement of enzymac acvies; monitoring lipid transport across membranes or at biological interfaces; or monitoring lipid dynamics.

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Sphingomyelin is critical in organizing phosphoinositide dynamics during phagocytic uptake of Mycobacterium tuberculosis

Cited by 5 publications

References 27 publications

Lipoarabinomannan as a Point-of-Care Assay for Diagnosis of Tuberculosis: How Far Are We to Use It?

Lipoarabinomannan as a Point-of-Care Assay for Diagnosis of Tuberculosis: How Far Are We to Use It?

Glycolipids: Linchpins in the Organization and Function of Membrane Microdomains

Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

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