Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

Cebolla, Vicente L.; Jarne, Carmen; Vela, Jesús; Garriga, Rosa; Membrado, Luis; Galbán, Javier

doi:10.1080/10826076.2020.1866600

Cited by 12 publications

(18 citation statements)

References 136 publications

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“…TLC/MALDI enables the analysis of complex organic mixtures [253,254]. Previous reports indicate that this approach is effective in the identification of active compounds of plant origin, such as lipids or flavonoids [255,256]. The use of "direct analysis in real-time" (DART) in conjunction with TLC also seems to be an interesting approach.…”

Section: Gas Chromatography (Gc)mentioning

confidence: 99%

Comprehensive Study of Si-Based Compounds in Selected Plants (Pisum sativum L., Medicago sativa L., Triticum aestivum L.)

et al. 2023

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This review describes the role of silicon (Si) in plants. Methods of silicon determination and speciation are also reported. The mechanisms of Si uptake by plants, silicon fractions in the soil, and the participation of flora and fauna in the Si cycle in terrestrial ecosystems have been overviewed. Plants of Fabaceae (especially Pisum sativum L. and Medicago sativa L.) and Poaceae (particularly Triticum aestivum L.) families with different Si accumulation capabilities were taken into consideration to describe the role of Si in the alleviation of the negative effects of biotic and abiotic stresses. The article focuses on sample preparation, which includes extraction methods and analytical techniques. The methods of isolation and the characterization of the Si-based biologically active compounds from plants have been overviewed. The antimicrobial properties and cytotoxic effects of known bioactive compounds obtained from pea, alfalfa, and wheat were also described.

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Section: Gas Chromatography (Gc)mentioning

confidence: 99%

Comprehensive Study of Si-Based Compounds in Selected Plants (Pisum sativum L., Medicago sativa L., Triticum aestivum L.)

et al. 2023

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“…One of the areas of interest in current analytical chemistry is lipid analysis. Cebolla et al (2021) have recently presented a review on HPTLC contribution to lipid analysis in complex matrices during the period 2010-2021, when hyphenation of TLC to MS methods had experienced significant growth. Authors demonstrated that HPTLC separation of lipids on classes allowed the subsequent analysis by MALDI-TOF, resolving the difficulties in the analysis of acidic glycerophospholipid species due to ion suppression by phosphatidylcholine species.…”

Section: Applicationsmentioning

confidence: 99%

Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques

2021

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The combination of planar chromatography with desorption/ionization mass-spectrometry (MS) techniques provides chemists with unique tools for fast and simple separation of mixtures followed by the detection of analytes by the most powerful analytical method. Since its introduction in the early 1990s, thin-layer chromatography (TLC)/matrix-assisted mass spectrometry (MALDI) has been used for the analysis of a wide range of analytes, including natural and synthetic organic compounds. Nowadays, new desorption/ionization approaches have been developed and applied in conjunction with planar chromatography competing with MALDI. This review covers recent developments in the combination of TLC with various desorption/ionization MS methods which were made in recent several years.

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“…The plates further contain thinner layers and the surface is smoother [ 55 ]. These features altogether reduce sample quantity requirements and allow for more economical and faster separation with reduced diffusion rates [ 55 – 57 ]. This results in higher sensitivity, more efficient separation and better sample resolution, and also positively affects reproducibility [ 55 – 57 ].…”

Section: Introductionmentioning

confidence: 99%

Quantifying yeast lipidomics by high-performance thin-layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics

Meyer,

Knittelfelder,

Smolnig

et al. 2024

Microb Cell

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Lipidomic analysis in diverse biological settings has be-come a frequent tool to increase our understanding of the processes of life. Cellular lipids play important roles not only as being the main components of cellular membranes, but also in the regulation of cell homeostasis as lipid signaling molecules. Yeast has been harnessed for biomedical research based on its good conservation of genetics and fundamental cell organisation principles and molecular path-ways. Further application in so-called humanised yeast models have been developed which take advantage of yeast as providing the ba-sics of a living cell with full control over heterologous expression. Here we present evidence that high-performance thin-layer chroma-tography (HPTLC) represents an effective alternative to replace cost intensive mass spectrometry-based lipidomic analyses. We provide statistical comparison of identical samples by both methods, which support the use of HPTLC for quantitative analysis of the main yeast lipid classes.

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Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

Cited by 12 publications

References 136 publications

Comprehensive Study of Si-Based Compounds in Selected Plants (Pisum sativum L., Medicago sativa L., Triticum aestivum L.)

Comprehensive Study of Si-Based Compounds in Selected Plants (Pisum sativum L., Medicago sativa L., Triticum aestivum L.)

Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques

Quantifying yeast lipidomics by high-performance thin-layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics

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