Sphingomonas aerophila sp. nov. and Sphingomonas naasensis sp. nov., isolated from air and soil, respectively

Abstract

Two strains, designated 5413J-26T and KIS18-15T, were isolated from the air and forest soil, respectively, in South Korea. Cells of the two strains were Gram-stain-negative, aerobic, polar-flagellated and rod-shaped. According to the phylogenetic tree, strains 5413J-26T and KIS18-15T fell into the cluster of Sphingomonas sensu stricto. Strain 5413J-26T showed the highest sequence similarities with Sphingomonas trueperi LMG 2142T (96.6 %), Sphingomonas molluscorum KMM 3882T (96.5 %), Sphingomonas azotifigens NBRC 15497T (96.3 %) and Sphingomonas pituitosa EDIVT (96.1 %), while strain KIS18-15T had the highest sequence similarity with Sphingomonas soli T5-04T (96.8 %), Sphingomonas pituitosa EDIVT (96.6 %), Sphingomonas leidyi ATCC 15260T (96.6 %), Sphingomonas asaccharolytica NBRC 15499T (96.6 %) and Sphingomonas koreensis JSS26T (96.6 %). The 16S rRNA gene sequence similarity between strains 5413J-26T and KIS18-15T was 95.4 %. Ubiquinone 10 was the predominant respiratory quinone and homospermidine was the major polyamine. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and several unidentified phospholipids and lipids. The main cellular fatty acids (>10 % of the total fatty acids) of strain 5413J-26T were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C14 : 0 2-OH, and those of strain KIS18-15T were summed feature 8 and C16 : 0. Based on the results of 16S rRNA gene sequence analysis, and physiological and biochemical characterization, two novel species with the suggested names Sphingomonas aerophila sp. nov. (type strain 5413J-26T = KACC 16533T = NBRC 108942T) and Sphingomonas naasensis sp. nov. (type strain KIS18-15T = KACC 16534T = NBRC 108943T) are proposed.

Sphingomonas faucium sp. nov., isolated from canyon soil

“…S3). However, the polar lipid profile of strain E62-3 T lacked sphingoglycolipid which was similar to Sphingomonas aerophila and Sphingomonas naasensis (Kim et al, 2014). 6.0-9.0 6.0-9.0 5.0-9.0 6.0-11.0 NaCl (%, w/v) tolerance 0.5 3 5 0.5 Kaur et al (2012); b, Chen et al (2013); c, Yasir et al (2010).…”

“…The major polyamine homospermidine was found in the genera Sphingomonas and Sphingosinicella, while spermidine was present in the genera Novosphingobium, Sphingobium, and Sphingopyxis Busse et al, 2003;Maruyama et al, 2006). Members of the genus Sphingomonas were isolated from various samples, such as soil, air, water, marine bivalve specimen and a lead-zinc mine (Lee et al, 2001;Romanenko et al, 2007;Lin et al, 2012;Son et al, 2013;Feng et al, 2014;Kim et al, 2014). All strains in the genus are Gram-stain-negative, aerobic, rod-shaped and catalase-positive Chen et al, 2012a;Kaur et al, 2012).…”

Detection of Legionella Species and the Influence of Precipitation on the Amount of Legionella DNA Present in Aerosols from Outdoor Sites Near Asphalt Roads in Toyama Prefecture, Japan, with Microbiome Analysis

“…16S rRNA gene amplicon sequencing revealed that Sphingomonas was the most frequently detected genus in the air samples. This genus has been found in various environments such as soil, water, clinical specimens, air, and other locations [30][31][32], indicating the opportunity for the bacteria to be released into the air. Pseudomonas and Achromobacter were signi cantly more abundant in air samples from bathrooms and other indoor sites, respectively.…”

Sphingomonas profundi sp. nov., isolated from deep-sea sediment of the Mariana Trench

“…net/ sphingomonas. html), the genus Sphingomonas contains 131 recognized species isolated from various natural sources, such as air, soil [7][8][9], groundwater [10], marine bivalves [11] and freshwater sediments [12]. The present study reports on the taxonomic characterization of a Sphingomonas-like bacterial strain (designated LMO-1 T ) isolated from marine sediment.…”