Sphingomonadaceae: Protective against breast cancer?

Lawani-Luwaji, Ebidor Ufoumanefe; Tolulope, Alade

doi:10.1186/s42269-020-00447-0

“…Of the top 30 genera, Sphingobium was almost only exclusively discovered in healthy individuals. Recent studies have suggested its potential protective role in breast cancer, because of its ability to metabolize oestrogen [53, 54]. Aggregatibacter was more abundant in tumorous patients in our study.…”

Section: Discussionsupporting

confidence: 54%

Variations in oral microbiome and its predictive functions between tumorous and healthy individuals

Yang

¹

,

He

²

,

Zhou

³

et al. 2022

Journal of Medical Microbiology

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Introduction. The oral cavity is one of the largest reservoirs of microorganisms and many pathogenic bacteria have been shown to be associated with the aetiology of oral cancers. Gap Statement. Owing to the complexity of oral microbial communities and their unclear relationship with oral cancer, identification of specific bacteria which contribute to oral cancer is a key imperative. Aim. To compare and investigate the variations in the composition of the bacterial microbiome and its functions between patients with oral tumorous lesions and healthy subjects. Methodology. Twenty-seven samples from individuals with oral tumours (five oral benign tumours and 22 oral squamous cell carcinomas) and 15 samples from healthy subjects were collected. Genomic DNA was extracted and the V3–V5 region of the 16S rRNA gene was sequenced. Subsequently, bioinformatic assessment was conducted using QIIME2, PICRUSt and linear discriminant analysis effect size analyses (LEfSe). Results. The oral microbiota was composed mainly of the phyla Proteobacteria (31.76 %, 35.00 %), Bacteroidetes (30.13 %, 25.13 %) and Firmicutes (23.92 %, 17.07 %) in tumorous and healthy individuals, respectively. Neisseria , Prevotella , Fusobacterium , Streptococcus , Capnocytophaga , Veillonella , Haemophilus , Prevotella , Porphyromonas and Leptotrichia were the most abundant genera. Alpha diversity in the tumour group was significantly greater than that in the healthy group (P<0.05). Differential analysis of microbes between groups demonstrated a significantly higher number of Neisseria , Veillonella , Streptococcus , Leptotrichia , Lautropia , Sphingopyxis , Sphingobium , Tannerella , Actinomyces and Rothia in healthy controls compared with the tumour group. However, the genera Treponema , Micrococcus , Pseudomonas , Janthinobacterium , Parvimos, Loktanella , Staphylococcus , Acinetobacter , Catonella , Aggregatibacter and Propionibacterium were significantly higher in the tumour group. Pathways related to cancers, cell motility, environmental adaptation, metabolism and signal transduction were enhanced in the tumour group, while functions associated with immune system diseases, replication, repair and translation were significantly enhanced in the healthy group. Conclusion. Variations in the oral microbiota and its functions showed a correlation with oral tumours. The tumour group showed an increased abundance of some multi-drug-resistant and periodontitis-related pathogens. The significantly altered microbiotas may serve as potential biomarkers or inform combination therapy for oral tumours.

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“…2B, C ). The family Sphingomonadaceae , which includes Sphigomonas , was previously suggested to be protective against breast cancer [ 45 ]. The highlighted bacterium in that study was a member of the genus Sphingobium , which we find in 18.3% of healthy esophagi but only a single ESCA sample (Fig.…”

Section: Resultsmentioning

confidence: 99%

Microbial gene expression analysis of healthy and cancerous esophagus uncovers bacterial biomarkers of clinical outcomes

Schäffer,

Li,

Elbasir

et al. 2023

ISME Communications

0

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Local microbiome shifts are implicated in the development and progression of gastrointestinal cancers, and in particular, esophageal carcinoma (ESCA), which is among the most aggressive malignancies. Short-read RNA sequencing (RNAseq) is currently the leading technology to study gene expression changes in cancer. However, using RNAseq to study microbial gene expression is challenging. Here, we establish a new tool to efficiently detect viral and bacterial expression in human tissues through RNAseq. This approach employs a neural network to predict reads of likely microbial origin, which are targeted for assembly into longer contigs, improving identification of microbial species and genes. This approach is applied to perform a systematic comparison of bacterial expression in ESCA and healthy esophagi. We uncover bacterial genera that are over or underabundant in ESCA vs healthy esophagi both before and after correction for possible covariates, including patient metadata. However, we find that bacterial taxonomies are not significantly associated with clinical outcomes. Strikingly, in contrast, dozens of microbial proteins were significantly associated with poor patient outcomes and in particular, proteins that perform mitochondrial functions and iron-sulfur coordination. We further demonstrate associations between these microbial proteins and dysregulated host pathways in ESCA patients. Overall, these results suggest possible influences of bacteria on the development of ESCA and uncover new prognostic biomarkers based on microbial genes. In addition, this study provides a framework for the analysis of other human malignancies whose development may be driven by pathogens.

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“…Conversely, Sphingobium may play a protective role. Due to its extra ability to metabolize estrogen, it may have favorable influence on outcomes in breast cancer, especially estrogen-positive breast cancer [78,79]. However, the exact roles the pathogens may play in bladder cancer pathology remain to be seen.…”

Section: Discussionmentioning

confidence: 99%

Microbiome Sex-Related Diversity in Non-Muscle-Invasive Urothelial Bladder Cancer

Bilski,

Żeber-Lubecka,

Kulecka

et al. 2024

CIMB

0

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Sex-specific discrepancies in bladder cancer (BCa) are reported, and new studies imply that microbiome may partially explain the diversity. We aim to provide characterization of the bladder microbiome in both sexes diagnosed with non-muscle-invasive BCa with specific insight into cancer grade. In our study, 16S rRNA next-generation sequencing was performed on midstream urine, bladder tumor sample, and healthy-appearing bladder mucosa. Bacterial DNA was isolated using QIAamp Viral RNA Mini Kit. Metagenomic analysis was performed using hypervariable fragments of the 16S rRNA gene on Ion Torrent Personal Genome Machine platform. Of 41 sample triplets, 2153 taxa were discovered: 1739 in tumor samples, 1801 in healthy-appearing bladder mucosa and 1370 in midstream urine. Women were found to have smaller taxa richness in Chao1 index than men (p = 0.03). In comparison to low-grade tumors, patients with high-grade lesions had lower bacterial diversity and richness in urine. Significant differences between sexes in relative abundance of communities at family level were only observed in high-grade tumors.

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Sphingomonadaceae: Protective against breast cancer?

Cited by 6 publications

References 27 publications

Variations in oral microbiome and its predictive functions between tumorous and healthy individuals

Variations in oral microbiome and its predictive functions between tumorous and healthy individuals

Microbial gene expression analysis of healthy and cancerous esophagus uncovers bacterial biomarkers of clinical outcomes

Microbiome Sex-Related Diversity in Non-Muscle-Invasive Urothelial Bladder Cancer

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