Spherical indentation of free-standing acellular extracellular matrix membranes

Cloonan, Aidan J.; O'Donnell, Michael R.; Lee, William T.; Walsh, Michael T.; Barra, Eamonn De; McGloughlin, Timothy M.

doi:10.1016/j.actbio.2011.08.003

Cited by 17 publications

(18 citation statements)

References 52 publications

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“…The aim of this study was to introduce the SIS of sheep as a new source of ECM‐based scaffolds. Characterization of this layer after decellularization with chemical reagents, SDS and TX‐100, represents superior quality compared to commercial and noncommercial scaffolds for applications in tissue engineering and regenerative medicine . ECM‐based scaffolds can be biocompatible, biodegradable, and porous scaffolds and provide a suitable in vivo environment for cell proliferation, adhesion, and differentiation as well as protein expression, tissue regeneration, and angiogenesis .…”

Section: Discussionmentioning

confidence: 99%

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Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix‐based scaffold for tissue engineering

Rashtbar

Hadjati

et al. 2017

J Biomed Mater Res

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Extracellular matrix-based scaffolds derived from mammalian tissues have been used in tissue engineering applications. Among all the tissues, decellularized small intestine submucosal layer (SIS) has been recently investigated for its exceptional characteristics and biocompatibilities. These investigations have been mainly focused on the decellularized porcine SIS; however, there has not been any report on ovine SIS (OSIS) layer. In this study, OSIS was decellularized and its physical, chemical, and morphological properties were evaluated. Decellularization was carried out using chemical reagents and various physical conditions. The effects of different conditions were evaluated on histological and biomechanical properties, quality of residual DNA, GAPDH gene expression, and biocompatibility. Results revealed satisfactory decellularization of OSIS which could be due to its thin thickness. Mechanical properties, structural form, and glycosaminoglycan contents were preserved in all the decellularized groups. In SDS-treated groups, further cells and DNA residues were removed compared to the groups treated with Triton X-100 only. No toxicity was observed in all treatments, and viability, expansion, and cell proliferation were supported. In conclusion, our results suggest that OSIS decellularized scaffold could be considered as an appropriate biological scaffold for tissue engineering applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 933-944, 2018.

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Section: Discussionmentioning

confidence: 99%

“…Mechanical strength properties depend on many factors, such as age of the animal, diet, race, and so forth. The type of decellularization process and final sterilization are considered the most important factors …”

Section: Discussionmentioning

confidence: 99%

Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix‐based scaffold for tissue engineering

Rashtbar

Hadjati

et al. 2017

J Biomed Mater Res

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“…Loading at a high frequency is relevant to the materials viscoelastic response to fast -loading events and is also useful as an accelerated evaluation of the ability of the phantom materials to sustain repeated loading. 31,32 Topography Analysis…”

Section: Dynamic Mechanical Analysismentioning

confidence: 99%

3D-Printed Tissue-Mimicking Phantoms for Medical Imaging and Computational Validation Applications

Cloonan

Shahmirzadi

et al. 2014

3D Printing and Additive Manufacturing

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“…Its preparation steps include manual manipulation of the harvested bladder tissue, chemical decellularisation, lyophilisation and sterilisation [5]. Although these processing steps are necessary for constructing a biocompatible tissue-engineered biomaterial, an overriding clinical concern is damage to the scaffold's mechanical properties and ultrastructure during the invasive preparation process [6,7]. Specific collagenous arrangements are frequently disrupted when ECMs are exposed to chemical reagents leading to significant decreases in mechanical strength.…”

Section: Introductionmentioning

confidence: 99%

Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds

Callanan

Davis

Walsh

et al. 2012

Medical Engineering & Physics

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Spherical indentation of free-standing acellular extracellular matrix membranes

Cited by 17 publications

References 52 publications

Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix‐based scaffold for tissue engineering

Characterization of decellularized ovine small intestine submucosal layer as extracellular matrix‐based scaffold for tissue engineering

3D-Printed Tissue-Mimicking Phantoms for Medical Imaging and Computational Validation Applications

Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds

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