Spezifische IgE-Werte gegen Aero- und Nahrungsmittelallergene im Vergleich: Dr. Fooke ALLERG-O-LIQ und Pharmacia CAP-System

Kleine-Tebbe, J.; Breuer, Klaus; Lepp, Ute; Vieths, Stefan; Worm, Margitta; Kunkel, G.; Wahn, Ulrich; Lau, Seng Fong

doi:10.5414/alp27129

Cited by 6 publications

(5 citation statements)

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“…According to the studies by various authors, the consistency of ALLERG-O-LIQ with ImmunoCAP results (the most common method used in allergy diagnosis) for quantitative or semi-quantitative determination of various allergens has been verified [22-24], although the correlation is stronger for aeroallergens than for food allergens.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study

Sun

Zheng

Zhang

et al. 2014

Multidiscip Respir Med

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BackgroundThe distribution of allergens may vary with different geographic areas, suggesting the importance of local epidemiological data to support evidence-based prevention and management of allergic diseases. We investigated the distribution of common allergens in allergic patients in Guangzhou, southern China.Methods7,047 patients with allergic symptoms were examined for serum sIgE to 15 common allergens in this region, based on the protocol of reversed enzyme allergosorbent test.Results4,869 (69.09%) of the subjects tested positive for sIgE to at least one of the 15 common allergens. There was no statistical difference in the overall rate of positive sIgE detection between males (3128/4523, 69.16%) and females (1741/2524, 68.98%). Der pteronyssinus and Der farinae were the most common aeroallergens, while eggs and cow’s milk the most common food allergens, responsible for higher positive rates of sIgE responses. A good correlation in positive sIgE response was found between Der pteronyssinus and Der farinae. By age-group analysis, we noted several peaks of sensitization to certain allergens: Der pteronyssinus, Der farinae, and Blomiatropicalis at age between 9 and 12; Blattellagermanica and mosquito at age between 15 and 18, cow’s milk before age 3; eggs and flour at age between 3 and 6; crabs and shrimps at age between 12 and 15. Along with older age, there was an ascending tendency in the overall positive rate of sIgE response to house dust mites among subjects who tested positive for sIgE to eggs or cow’s milk.ConclusionsDer pteronyssinus, Der farinae, cow’s milk, and eggs are major allergens in Guangzhou. Sensitization to eggs and cow’s milk is more common at younger age, and then gives place to the increasing prevalence of sensitization to Der pteronyssinus and Der farinae at older age. Such a sequence of events may be a result of allergy march. Knowledge on the prevalence of allergen sensitization in different age groups would help early diagnosis and intervention of allergic diseases in this large geographical region.

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Section: Discussionmentioning

confidence: 99%

Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study

Sun

Zheng

Zhang

et al. 2014

Multidiscip Respir Med

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“…In recent years, sIgE screening and profile tests have been developed using different protocols. In 2004, comparison between ALLERG-O-LIQ and the ImmunoCAP System showed good agreement for inhalant allergens and moderate agreement for food allergens [ 7 ]. In a recent study, ALFA has been evaluated by visual result interpretation and found in good qualitative agreement to SPT (90.8%), to ImmunoCAP (96.7%), and to ALLERG-O-LIQ (98.3%) [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Novel Rapid Test System for the Detection of Allergic Sensitization to Timothy Grass Pollen against Established Laboratory Methods and Skin Prick Test

Lucassen

Schulte-Pelkum

Csuvarszki³

et al. 2010

Journal of Allergy

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Type I hypersensitivity is driven by allergen specific immunoglobulin E (sIgE) and thus sIgE represents a marker for modern allergy diagnosis. Recently, a rapid assay for the detection of sIgE, termed as (Allergy Lateral Flow Assay) ALFA, has been developed. The objective of our study is the evaluation of a scanner-based system for the semiquantitative interpretation of ALFA results. Agreement to Skin Prick Test (SPT, Allergopharma), ALLERG-O-LIQ System (Dr. Fooke), and ImmunoCAP (Phadia) was investigated using 50 sera tested for specific IgE to timothy grass pollen (g6). 35/50 sera were positive by SPT, ALLERG-O-LIQ, and ImmunoCAP. Excellent agreement was observed between ALFA results and SPT, ImmunoCAP, and ALLERG-O-LIQ. Area under the curve (AUC) values were found at 1.0, and 100% sensitivity and specificity was found versus all other methods. Visual- and scanner-based interpretation of the ALFA results revealed excellent agreement.

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“…The ALLERG‐O‐LIQ is based on the ‘reversed enzyme allergo sorbent test (REAST)’ using antihuman IgE coated‐microtiter plates in combination with biotinylated reagents . In contrast, in the ImmunoCAP system (direct immunoassay), the allergens are bound to a solid phase .…”

Section: Methodsmentioning

confidence: 99%

Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains

Offermann

Plum

Hübner

et al. 2016

Allergy

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Human sera are the first choice as controls for diagnostic applications such as immunoassays, but are limited regarding availability, varying quality, and high costs. In this study, we aimed to circumvent these limitations by the use of a chimeric adaptor molecule comprising the extracellular domains of the human FcγRI (CD64) fused with human IgE Fc domains (CD64-IgE Fc). Allergen-specific antibodies were produced in rabbits using eight different allergens, extracts, and allergen mixtures including mites, pollen, drugs, and food. Preincubation of polyclonal IgG with CD64-IgE Fc established allergen-specific artificial sera that showed comparable results for more than 20 allergens and allergen extracts in three diagnostic systems for the determination of specific IgE. The agreement for these artificial sera is within ±1 radioallergosorbent test (RAST) class. Hence, rabbit IgG complexed with the IgG-specific CD64-IgE Fc adaptor molecule could provide a substitute for human reference sera with specificity for virtually any protein of interest.

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Spezifische IgE-Werte gegen Aero- und Nahrungsmittelallergene im Vergleich: Dr. Fooke ALLERG-O-LIQ und Pharmacia CAP-System

Cited by 6 publications

References 0 publications

Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study

Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study

Evaluation of a Novel Rapid Test System for the Detection of Allergic Sensitization to Timothy Grass Pollen against Established Laboratory Methods and Skin Prick Test

Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains

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