Spermidine enhanced the antioxidant capacity of rice seeds during seed aging

Hu, Qi; Chen, Mo Xian; Song, Tao; Cheng, Chen; Tian, Yuan; Hu, Jin; Zhang, Jianhua

doi:10.1007/s10725-020-00613-4

Cited by 13 publications

(10 citation statements)

References 68 publications

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“…Then, the supernatant obtained following centrifugation at 6000× g for 10 min was poured into a 50 mL volumetric flask, and the amylase stock solution was made up to volume with distilled water. Then, 1 mL of these samples was diluted in 50 mL of distilled water as a diluent to determine the (α + β) amylase activities, which were measured using 3,5-dinitrosalicylic acid colorimetry, as described by Hu et al [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…Seed germination is a physiological activity that is regulated by endogenous and exogenous factors [ 15 , 16 ]. This complex physiological process involves activities such as water absorption, storage degradation, seed respiration, transcription product (mRNA) synthesis, and mitochondrial repair and proliferation, all of which require an adequate energy supply [ 15 , 17 , 18 ]. The energy supply for seed germination and emergence comes from the catabolism of stored substances in the seed before the seedlings acquire photosynthetic capacity [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…It was found that starch decomposition is blocked during germination of aged seeds [ 45 , 46 ]. A few previous studies have used Spd to improve seed aging vigor, but largely, it has been used to improve the activity of antioxidant enzymes and related gene expression, ability to scavenge reactive oxygen species, and seed vigor [ 2 , 18 ]. Hitherto, only a few studies have assessed the effect of Spd on sugar metabolism during the germination of aged seeds, and even fewer studies have done so in sorghum seeds.…”

Section: Introductionmentioning

confidence: 99%

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Exogenous Spermidine Promotes Germination of Aged Sorghum Seeds by Mediating Sugar Metabolism

Zhang

Wan

et al. 2022

Plants

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Starch, a substance stored in seeds, is the main source of energy for germination in sorghum seeds. However, as the seeds age, the catabolism of seed starch is affected, thereby seriously damaging germination ability. In this study, we aimed to understand how exogenous spermidine promoted germination in aged sorghum seed. Our phenotypic analysis indicated that exogenous spermidine not only significantly improved the germination rate, germination potential, germination index, and vigor index of aged seeds, but also increased the root and shoot length after germination. Further, physiological analysis showed that exogenous spermidine increased the content of soluble sugar by upregulating the activity of amylase and sucrose invertase. Exogenous spermidine also improved the activities of key enzymes in glycolysis, the tricarboxylic acid cycle, and the pentose phosphate pathway of aged sorghum seeds. Interestingly, exogenous spermidine protected the mitochondrial structure of aged seeds, which was consistent with the increase in the respiration rate and ATP content during seed germination. Moreover, qRT-PCR analysis revealed that exogenous spermidine induced the expression of key genes related to starch and sugar metabolism in aged sorghum seeds. In conclusion, our study demonstrated that exogenous spermidine promoted aged sorghum seed germination by regulating starch and sugar metabolism.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exogenous Spermidine Promotes Germination of Aged Sorghum Seeds by Mediating Sugar Metabolism

Zhang

Wan

et al. 2022

Plants

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“…Franck et al (1995) reported in Prunus avium that in contrast to normal shoots, the hyperhydric shoots showed higher activity of H 2 O 2 generating enzyme, superoxide dismutase and lower activity of H 2 O 2 detoxifying enzymes viz., catalase, peroxidase, ascorbate peroxidase, glutathione reductase, and dehydro-ascorbate reductase. A recent report suggested that treatment of Spd alleviated accelerated ageing process of rice seedlings through enhanced scavenging activity of ROS (Hu et al 2020). It suggests that the exogenous supply of PAs regulate abiotic stress condition of plants (Tabart et al 2015).…”

Section: Effect Of Polyamines On Hh Reversionmentioning

confidence: 98%

Influence of polyamines on hyperhydricity reversion and its associated mechanism during micropropagation of China pink (Dianthus chinensis L.)

Sreelekshmi

Siril

2020

Physiol Mol Biol Plants

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Hyperhydricity (HH) is a physiological disorder that frequently occurs in plant tissue cultures, affecting healthy growth and development of clonal plants. The primary cultures raised in Murashige and Skoog (MS) medium supplemented with 2.5 lM N 6 -benzyladenine (BA) produced normal microshoot (6.3 shoots/ culture) with least HH. However, the third subculture onwards, HH becomes a major problem. The role of ethylene on HH induction through stomatal closure mechanism were proved by the supplementation of ethephon (5 lM) in the culture medium containing 2.5 lM BA. In the present study, the application of polyamines (putrescine, spermidine, or spermine) to minimize the HH was examined. Supplementation of 5 lM spermine in MS medium significantly reduced the percentage of HH to 0.33%, in contrast to control (100%), while a maximum number of healthy reverted shoots (11.0) were observed in 5 lM spermidine treatment. The addition of polyamines effectively reduced H 2 O 2 content (50%) characterized by increased chlorophyll content with proper stomatal morphology. The relative gene expression profile of ethylene biosynthesis genes, 1-Aminocyclopropane-1-carboxylase synthase (ACS1) and 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO1) at 5 lM spermine added medium was 1.09 and 1.3 over normal (1) or HH cultures (1.93 and 2.53) respectively, and thus directed restoration of normal morphology of shoots. The present finding in brief, forward a novel method to regulate HH in terms of endogenous ethylene by adopting polyamines exposure and the procedure can be applied to many other plants facing similar HH problems.

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“…Under nitrogen-saturated conditions, the loss of functional molecules, especially vitamin E, was reduced [ 170 ]. Moreover, new approaches aiming to improve seed storage include the invigoration of seeds with, i.e., exogenous antioxidants [ 171 , 172 , 173 ] or usage of nanoparticles for seed protection against microbial infections and alteration of deteriorative physiological processes through ROS scavenging. As a result, strategies incorporating the usage of nanoparticles have the potential for beneficial impact on plant growth, development and stress resistance [ 7 , 174 , 175 ].…”

Section: Future Possibility Of Improving Storability Of Seeds With Co...mentioning

confidence: 99%

Choosing the Right Path for the Successful Storage of Seeds

Trusiak

Plitta-Michalak

Michalak

2022

Plants

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Seeds are the most commonly used source of storage material to preserve the genetic diversity of plants. However, prior to the deposition of seeds in gene banks, several questions need to be addressed. Here, we illustrate the scheme that can be used to ensure that the most optimal conditions are identified to enable the long-term storage of seeds. The main questions that need to be answered pertain to the production of viable seeds by plants, the availability of proper protocols for dormancy alleviation and germination, seed tolerance to desiccation and cold storage at −20 °C. Finally, it is very important to fully understand the capability or lack thereof for seeds or their explants to tolerate cryogenic conditions. The proper storage regimes for orthodox, intermediate and recalcitrant seeds are discussed.

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Spermidine enhanced the antioxidant capacity of rice seeds during seed aging

Cited by 13 publications

References 68 publications

Exogenous Spermidine Promotes Germination of Aged Sorghum Seeds by Mediating Sugar Metabolism

Exogenous Spermidine Promotes Germination of Aged Sorghum Seeds by Mediating Sugar Metabolism

Influence of polyamines on hyperhydricity reversion and its associated mechanism during micropropagation of China pink (Dianthus chinensis L.)

Choosing the Right Path for the Successful Storage of Seeds

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