Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters

Figueroa, Elías; Risopatrón, Jennie; Sánchez, Raúl; Isachenko, Evgenia; Merino, O.; Isachenko, Vladimir; Valdebenito, Iván

doi:10.1016/j.aquaculture.2012.10.019

Cited by 82 publications

(67 citation statements)

References 57 publications

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“…The samples show an activation time of 180 ± 10 s and decay at 50%, similar to results from a previous study [25]. The samples were agitated daily and aerated by injecting compressed oxygen to prevent mortality of the sperm cells (which are not motile for the entire conservation period) and preserved for 20 days for periodic experiments [26].…”

Section: Collection and Handling Of Semen Samplessupporting

confidence: 56%

“…The samples utilized for observation consisted of cells with size 2 µM. After focusing the sample, 20 µl of Powermilt (1×) activation solution [26] was applied at the edge of the drop sample to obtain interface, and activate sperm cell movement was observed. The samples were then observed at the interface of the solutions to record the activation of the spermatozoa, the border between solutions was in focus when the Powermilt was added, all of which exhibits a low-cost, easy to replicate model and in many laboratories.…”

Section: Observation Of the Samples Under A Microscopementioning

confidence: 99%

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Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

Parodí

Ramirez-Reveco²,

Guerra³

2015

ABB

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A spermiogramme is an analysis performed to assess the quality of semen. Motility parameters are primarily obtained by subjectively observing samples or by using complex systems, such as computer-assisted sperm analysis (CASA). Here, we describe an easy and low-cost analysis system for obtaining quantifiable kinetic observations using Salmo salar semen model. In this work, we observed and captured video images of both fresh and stored Atlantic salmon semen to describe the possibility of analysis using the ImageJ CASA plug-in application for the kinetic parameters obtained from the videos. The semen is exposed to "powermilt", a commercial activating solution, and the curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP) values are described. When the samples were activated after having been stored, differences were detected in sperm quality, using the low-cost plug-in application. However, this system was not able to detect small variations in the same recorded sample, suggesting limits in sample observation. The results indicate that it is possible to quantify the kinetic parameters of semen samples using a low-cost video system and free software.

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Section: Collection and Handling Of Semen Samplessupporting

confidence: 56%

Section: Observation Of the Samples Under A Microscopementioning

confidence: 99%

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

Parodí

Ramirez-Reveco²,

Guerra³

2015

ABB

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“…The procedure described by Figueroa et al . () was followed; all the fertility tests were carried out five times with 200 oocytes per replication. The sperm density used in all the treatments was 1·5 × 10 7 spermatozoa oocyte −1 .…”

Section: Methodsmentioning

confidence: 99%

Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

Figueroa

Valdebenito

Merino

et al. 2016

Journal of Fish Biology

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The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium + 1.3M dimethyl sulphoxide + 0.3M glucose + 2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as the treatment (T) and fresh semen as the control (F). Straws of 0·5 ml of sperm suspension were frozen in 4 cm of N2 L. They were thawed in a thermoregulated bath (40° C). After thawing, the percentage of spermatozoa with fragmented DNA [transferase dUTP (deoxyuridine triphosphate) nick-end labelling (TUNEL)], plasma membrane integrity (SYBR-14/PI) and mitochondrial membrane potential (ΔΨMMit, JC-1) were evaluated by flow cytometry and motility was evaluated by optical microscope under stroboscopic light. The fertilization rates of the control and treatment semen were tested at a sperm density of 1·5 × 10(7) spermatozoa oocyte(-1) , by observation of the first cleavages after 16 h incubation at 10° C. In the cryopreserved semen (T), the mean ± s.d. DNA fragmentation was 4·8 ± 2·5%; plasma membrane integrity 75·2 ± 6·3%; mitochondrial membrane potential 51·7 ± 3·6%; motility 58·5 ± 5·3%; curved line velocity (VCL ) 61·2 ± 17·4 µm s(-1) ; average-path velocity (VAP ) 50·1 ± 17·3 µm s(-1) ; straight-line velocity (VSL ) 59·1 ± 18·4 µm s(-1) ; fertilization rate 81·6 ± 1·9%. There were significant differences in the plasma membrane integrity, mitochondrial membrane potential, motility, fertilization rate, VCL , VAP and VSL compared with the controls (P < 0·05). Also the mitochondrial membrane potential correlated with motility, fertilization rate, VCL and VSL (r = 0·75; r = 0·59; r = 0·77 and r = 0·79, respectively; P < 0·05); and the fertilization rate correlated with VCL and VSL (r = 0·59 and r = 0·55, respectively).

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“…Sperm vitrification has been applied in freshwater fishes, and offspring have been produced from vitrified sperm samples of Russian Sturgeon Acipenser gueldenstaedtii (Andreev et al 2009), Channel Catfish Ictalurus punctatus (Cuevas-Uribe et al 2011a), the live-bearing Green Swordtail (Cuevas-Uribe et al 2011b), Rainbow Trout O. mykiss (Figueroa et al 2013), Atlantic Salmon (Figueroa et al 2015), and Eurasian Perch Perca fluviatilis (Kasa et al, in press). Our recent studies evaluated sperm vitrification in three marine species, Spotted Seatrout Cynoscion nebulosus , Red Drum Sciaenops ocellatus , and Red Snapper Lutjanus campechanus , in which sperm motility and membrane integrity were general indicators of gamete quality (Cuevas-Uribe et al 2015).…”

mentioning

confidence: 99%

“…In addition, males produce a very small volume (<500 μL) of sperm (Daniels 2000). Vitrification could offer a new way to protect this investment and reconstitute these valuable sex-reversed males as was demonstrated for neomales of Rainbow Trout (Figueroa et al 2013). …”

mentioning

confidence: 99%

Vitrification as an Alternative Approach for Sperm Cryopreservation in Marine Fishes

Cuevas‐Uribe

Hu²,

Daniels

et al. 2017

N American J Aquac

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The Southern Flounder Paralichthys lethostigma is a high-value species and a promising aquaculture candidate. Because sperm volume can be limited in this species (<500 μL), new sperm cryopreservation methods need to be evaluated. Vitrification is an alternative to conventional slow-rate freezing, whereby small volumes are cryopreserved at high cooling rates (>1,000°C/min). The goal of this work was to develop a standardized approach for vitrification of Southern Flounder sperm. The specific objectives were to (1) evaluate thawing methods and vitrification solutions, (2) evaluate the postthaw membrane integrity of sperm vitrified in different cryoprotectant solutions, (3) examine the relationship between membrane integrity and motility, and (4) evaluate the ability of vitrified sperm to fertilize eggs. From the vitrification solutions tested, the highest postthaw motility (28 ± 9% [mean ± SD]) and membrane integrity (11 ± 4%) was observed for 20% ethylene glycol plus 20% glycerol. There was no significant difference in postthaw motility of sperm thawed at 21°C or at 37°C. Fertilization from vitrified sperm in one trial yielded the same fertilization rate (50 ± 20%) as the fresh sperm control, while the sperm from the other two males yielded 3%. This is the first report of fertilization by vitrified sperm in a marine fish. Vitrification can be simple, fast, inexpensive, performed in the field, and, at least for small fishes, offers an alternative to conventional cryopreservation. Because of the minute volumes needed for ultrarapid cooling, vitrification is not presently suited as a production method for large fishes. Vitrification can be used to reconstitute lines from valuable culture species and biomedical models, conserve mutants for development of novel lines for ornamental aquaculture, and transport frozen sperm from the field to the repository to expand genetic resources.

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Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters

Cited by 82 publications

References 57 publications

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

Vitrification as an Alternative Approach for Sperm Cryopreservation in Marine Fishes

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Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters

Cited by 82 publications

References 57 publications

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (&lt;i&gt;Salmo salar&lt;/i&gt;)

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (&lt;i&gt;Salmo salar&lt;/i&gt;)

Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

Vitrification as an Alternative Approach for Sperm Cryopreservation in Marine Fishes

Contact Info

Product

Resources

About

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)