Spermatogenesis

Kretser, David Morritz de; Loveland, Kate L

doi:10.1016/b978-0-323-18907-1.00136-0

Cited by 17 publications

(12 citation statements)

References 383 publications

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“…Gonadotropin-releasing hormone (GnRH) receptor signaling plays a key role in the reproduction of vertebrates [108]. Pulsatile GnRH release from the hypothalamus activates GnRH receptors in gonadotrophs of the pituitary gland leading to the secretion of e.g., follicle-stimulating hormone (FSH) [108,109]. FSH binds receptors on SCs, stimulating various cellular mechanisms, e.g., protein synthesis by SCs and SC growth [109].…”

Section: Discussionmentioning

confidence: 99%

“…Pulsatile GnRH release from the hypothalamus activates GnRH receptors in gonadotrophs of the pituitary gland leading to the secretion of e.g., follicle-stimulating hormone (FSH) [108,109]. FSH binds receptors on SCs, stimulating various cellular mechanisms, e.g., protein synthesis by SCs and SC growth [109]. In addition to others, inhibin is produced and secreted by SCs, which negatively influences hormone release in the hypothalamus and pituitary gland [84].…”

Section: Discussionmentioning

confidence: 99%

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Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch

Hilbold

Distl

Hoedemaker

et al. 2020

Cells

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Male factor infertility is a problem in today’s society but many underlying causes are still unknown. The generation of a conditional Sertoli cell (SC)-specific connexin 43 (Cx43) knockout mouse line (SCCx43KO) has provided a translational model. Expression of the gap junction protein Cx43 between adjacent SCs as well as between SCs and germ cells (GCs) is known to be essential for the initiation and maintenance of spermatogenesis in different species and men. Adult SCCx43KO males show altered spermatogenesis and are infertile. Thus, the present study aims to identify molecular mechanisms leading to testicular alterations in prepubertal SCCx43KO mice. Transcriptome analysis of 8-, 10- and 12-day-old mice was performed by next-generation sequencing (NGS). Additionally, candidate genes were examined by qRT-PCR and immunohistochemistry. NGS revealed many significantly differentially expressed genes in the SCCx43KO mice. For example, GC-specific genes were mostly downregulated and found to be involved in meiosis and spermatogonial differentiation (e.g., Dmrtb1, Sohlh1). In contrast, SC-specific genes implicated in SC maturation and proliferation were mostly upregulated (e.g., Amh, Fshr). In conclusion, Cx43 in SCs appears to be required for normal progression of the first wave of spermatogenesis, especially for the mitosis-meiosis switch, and also for the regulation of prepubertal SC maturation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch

Hilbold

Distl

Hoedemaker

et al. 2020

Cells

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“…11,15 Because the number of Sertoli cells specifies the size of the testis, it is speculated that a certain amount of spermatogonia is supported by each Sertoli cell. 16,17 Throughout the spermatogenesis process, germ cells are in direct contact with the Sertoli cells.…”

Section: Introductionmentioning

confidence: 99%

“…However, attention has recently shifted to identifying the interaction between cells, because this information will be useful in clarifying the mechanism of cell-to-cell interaction. 11,16 Since the underlying mechanisms effects of DM on male reproductive function remain largely unknown, the evaluation of the correlation between Sertoli cells and spermatogonial cells can help in understanding the mechanism of disorder such as diabetes in the process of spermatogenesis. Although some methods are available for data abstraction.…”

Section: Introductionmentioning

confidence: 99%

Impaired spermatogenesis associated with changes in spatial arrangement of Sertoli and spermatogonial cells following induced diabetes

Sajadi

Dadras

Bayat

et al. 2019

J of Cellular Biochemistry

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The current study was conducted to assess the relationship between testicular cells in spermatogenesis, through which the production of healthy and mature sperm is essential. However, it seems necessary to obtain more information about the three‐dimensional pattern of the testis cells arrangement, which is directly related to the function of the testis after induction of diabetes. Twelve adult mice (28‐30 g) were assigned into two experimental groups: (1) control and (2) diabetic (40 mg/kg STZ). The epididymal sperm collected from the tail of the epididymis and testes samples were taken for stereology, immunocytochemistry and RNA extraction. Our data showed that diabetes could notably decrease the number of testicular cells, together with a reduction of total sperm count. In addition, the results from the second‐order stereology indicated the significant changes in the spatial arrangement of Sertoli cells and spermatogonial cells in the diabetic groups, in comparison with the control (P < .05). Moreover, the immunohistochemistry results showed a significant reduction in Sex‐determining Region Y (SRY) box 9 gene (SOX9), vimentin, occludin, and connexin‐43 positive cells in the diabetic groups compared with the control (P < .05). Furthermore, our data showed that the expression of steroidogenic acute regulatory protein steroidogenic acute regulatory protein (StAR) and peripheral benzodiazepine receptor peripheral benzodiazepine receptor (PBR) was significantly reduced in the diabetic groups, in comparison with the control (P < .05). These findings suggest that structural and functional changes of testis cells after induction of diabetes cause the alterations in the spatial arrangement of Sertoli and spermatogonial cells, ultimately influencing the normal spermatogenesis in mice.

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“…The remaining prospermatogonia not forming spermatids will continue to divide mitotically and produce spermatogonial stem cells, to ensure the continuation of spermatogonia supply. Finally, spermiogenesis involves microtubule growth, tail formation, and the tight packaging of DNA into condensed, protamine-bound chromatin (21).…”

Section: Foundations Of the Epigenetic Landscape Established In Primomentioning

confidence: 99%

Not just heads and tails: The complexity of the sperm epigenome

Gold

Jung

Corcés

2018

Journal of Biological Chemistry

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Transgenerational inheritance requires mechanisms by which epigenetic information is transferred via gametes. Canonical thought holds that mammalian sperm chromatin would be incapable of carrying epigenetic information as post-translational modifications of histones because of their replacement with protamine proteins. Furthermore, compaction of the sperm genome would hinder DNA accessibility of proteins involved in transcriptional regulation and genome architecture. In this Minireview, we delineate the paternal chromatin remodeling events during spermatogenesis and fertilization. Sperm chromatin is epigenetically modified at various time points throughout its development. This allows for the addition of environment-specific modifications that can be passed from parents to offspring.

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Spermatogenesis

Cited by 17 publications

References 383 publications

Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch

Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch

Impaired spermatogenesis associated with changes in spatial arrangement of Sertoli and spermatogonial cells following induced diabetes

Not just heads and tails: The complexity of the sperm epigenome

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