Sperm selection during ICSI treatments reduces single- but not double-strand DNA break values compared to the semen sample

Lara-Cerrillo, Sandra; Ribas-Maynou, Jordi; Rosado-Iglesias, Candela; Lacruz-Ruiz, Tania; Benet, J.; García-Peiró, Agustín

doi:10.1007/s10815-021-02129-w

Cited by 25 publications

(24 citation statements)

References 75 publications

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“…We observed that direct methods such as TUNEL and alkaline Comet, and indirect ones like SCSA, were correlated with progressive motility and sperm velocity. Previous studies in humans also reported that association (68)(69)(70), which may represent a positive bias for ICSI treatments, as the most motile sperm are selected (71).…”

Section: Discussionmentioning

confidence: 93%

Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage

et al. 2021

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Although sperm chromatin damage, understood as damage to DNA or affectations in sperm protamination, has been proposed as a biomarker for sperm quality in both humans and livestock, the low incidence found in some animals raises concerns about its potential value. In this context, as separate methods measure different facets of chromatin damage, their comparison is of vital importance. This work aims at analyzing eight techniques assessing chromatin damage in pig sperm. With this purpose, cryopreserved sperm samples from 16 boars were evaluated through the following assays: TUNEL, TUNEL with decondensation, SCSA, alkaline and neutral sperm chromatin dispersion (SCD) tests, alkaline and neutral Comet assays, and chromomycin A3 test (CMA3). In all cases, the extent of chromatin damage and the percentage of sperm with fragmented DNA were determined. The degree of chromatin damage and the percentage of sperm with fragmented DNA were significantly correlated (p < 0.05) in direct methods (TUNEL, TUNEL with decondensation, and alkaline and neutral Comet) and CMA3, but not in the indirect ones (SCD and SCSA). Percentages of sperm with fragmented DNA determined by alkaline Comet were significantly (p < 0.05) correlated with TUNEL following decondensation and CMA3; those determined by neutral Comet were correlated with the percentage of High DNA Stainability (SCSA); those determined by SCSA were correlated with neutral and alkaline SCD; and those determined by neutral SCD were correlated with alkaline SCD. While, in pigs, percentages of sperm with fragmented DNA are directly related to the extent of chromatin damage when direct methods are used, this is not the case for indirect techniques. Thus, the results obtained herein differ from those reported for humans in which TUNEL, SCSA, alkaline SCD, and alkaline Comet were found to be correlated. These findings may shed some light on the interpretation of these tests and provide some clues for the standardization of chromatin damage methods.

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Section: Discussionmentioning

confidence: 93%

Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage

et al. 2021

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“…Studies using the alkaline comet assay have reported associations between DNA fragmentation and % fertilization, embryo quality, implantation rate, clinical pregnancy and live birth [32][33]. In contrast, a smaller number of small studies with the neutral Comet assay have reported inconsistent associations between DNA integrity and ART outcomes [17,21,22,34]. Sperm with a high DNA fragmentation index (≥ 14%) was associated with a signi cantly lower fertilisation rate but not with the % of good embryos nor the pregnancy rate whereas in other studies DNA fragmentation was not associated with fertilisation rate [21,35] but with cleavage rate [21] and implantation rate [35].…”

Section: Discussionmentioning

confidence: 99%

DNA damage in prepared semen is associated with fertilisation rate in assisted reproduction technology (ART) treatment

Altakroni

Hunter

Horne

et al. 2023

Preprint

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Purpose This study aims to determine to what extent sperm DNA damage is associated with semen quality and assisted reproduction technology (ART) treatment outcomes. Methods 105 male partners of couples attending for infertility treatment were recruited and DNA integrity was measured by a neutral Comet assay and N7-methyldeoxyguanosine (N7-MedG) DNA levels by an immunoslotblot in sperm prepared by density gradient centrifugation for treatment use. Associations between measures of DNA damage (%tail DNA, proportion of sperm with either low (LDD) or high (HDD) levels of DNA damage, and N7-methylguanine levels), semen quality (concentration and motility) and ART outcomes (% oocytes fertilised, % embryo fragmentation, % cleavage, and birth outcome) were assessed. Results In the prepared sperm samples, DNA damage was significantly associated with semen quality and was lower than that in the original neat sample. % fertilisation was significantly negatively associated with N7-MedG levels, %HDD and % tail DNA and was positively associated with %LDD. % cleavage and live birth in fresh cycles were not associated with DNA damage but there was evidence that %LDD was lower, and %HDD higher, in couples with live births after a frozen embryo transfer cycle. Conclusion DNA damage can negatively impact on semen quality and fertilization rate but not embryo cleavage or live birth rate. These results suggest that the impact of sperm DNA damage on pregnancy outcomes appears in the early stage of embryo development.

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“…It can be used for foreign substance introduction, sperm injection, nuclear transfer, protein, peptide and genetic substance injection, drug delivery, etc. [57,[174][175][176] Cell puncture methods can be classified into ordinary glass needle puncture, piezoelectric-assisted puncture, Ros-drill puncture, and other methods.…”

Section: Cell Puncture Methodsmentioning

confidence: 99%

A Review of Single‐Cell Pose Adjustment and Puncture

Guo

Zhang

Jin

et al. 2022

Advanced Intelligent Systems

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Single‐cell manipulation technology is an essential method of cell research, with wide applications in biological engineering, medical engineering, agricultural engineering, and other precise manipulation fields. Cell pose adjustment and cell puncture are considered as two basic operations of single‐cell manipulation. Cell pose adjustment can be used for cell sorting, cell trapping, cell orientation, cell transportation, etc. It consists of direct contact manipulation methods and noncontact manipulation methods (e.g., mechanical contact method, electric field, optical field, magnetic field, acoustic field, and hydrodynamic methods). Cell puncture consists of ordinary glass needle puncture methods, piezoelectric‐assisted puncture methods, and rotational oscillatory drill puncture methods. It aims to achieve directional and quantified injection or sampling with minimal damage. Herein, the recent research progress on single‐cell pose adjustment and puncture methods is systematically summarized and discussed, which involves the basic mechanism, characteristics, limitations, and applications. Some potential directions for future research are proposed in accordance with the above analysis. It is hoped that this review can provide a reference for academic research and industrial applications of single‐cell manipulation technology.

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Sperm selection during ICSI treatments reduces single- but not double-strand DNA break values compared to the semen sample

Cited by 25 publications

References 75 publications

Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage

Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage

DNA damage in prepared semen is associated with fertilisation rate in assisted reproduction technology (ART) treatment

A Review of Single‐Cell Pose Adjustment and Puncture

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