Sperm quality analysis in XX, XY and YY males of the Nile tilapia (Oreochromis niloticus)

Gennotte, Vincent; François, Ekniel; Rougeot, Carole; Ponthier, Jérôme; Deleuze, Stéfan; Mélard, Charles

doi:10.1016/j.theriogenology.2012.02.002

Cited by 24 publications

(26 citation statements)

References 47 publications

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“…Taken together, these results strongly suggest that XX sex-reversed breeders exist in the wild where they reproduce and sire all or almost all-female offspring. Similarly, under controlled conditions androgen-induced XX males and XY or YY males of Nile tilapia were found to have quite similar male behavior and sperm quality [Gennotte et al, 2012]. Besides, our previous unpublished data revealed that under controlled conditions, progeny testing of 19 single hormonally-induced sex-reversed XX males sired progenies strongly skewed toward females but in some of them, unexpected XX males (1-25.5%) were identified.…”

Section: Consequences Of Sex Reversal In Wild Populationsmentioning

confidence: 65%

The Reversible Sex of Gonochoristic Fish: Insights and Consequences

Baroiller¹,

D'Cotta²

2016

Sex Dev

124

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Fish sex reversal is a means to understand sex determination and differentiation, but it is also used to control sex in aquaculture. This review discusses sex reversal in gonochoristic fish, with the coexistence of genetic and environmental influences. The different periods of fish sensitivity to sex reversal treatments are presented with the mechanisms implicated. The old players of sex differentiation are revisited with transcriptome data and loss of function studies following hormone- or temperature-induced sex reversal. We also discuss whether cortisol is the universal mediator of sex reversal in fish due to its implication in ovarian meiosis and 11KT increase. The large plasticity in fish for sex reversal is also evident in the brain, with a reversibility existing even in adulthood. Studies on epigenetics are presented, since it links the environment, gene expression, and sex reversal, notably the association of DNA methylation in sex reversal. Manipulations with exogenous factors reverse the primary sex in many fish species under controlled conditions, but several questions arise on whether this can occur under wild conditions and what is the ecological significance. Cases of sex reversal in wild fish populations are shown and their fitness and future perspectives are discussed.

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Section: Consequences Of Sex Reversal In Wild Populationsmentioning

confidence: 65%

The Reversible Sex of Gonochoristic Fish: Insights and Consequences

Baroiller¹,

D'Cotta²

2016

Sex Dev

124

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“…For example, Fitzpatrick, Henry, Liley, and Devlin (), analysed semen motility of Coho salmon neo‐males and suggested that the sex reversal procedure does not affect semen quality and maturation. Similarly, Gennotte, François, Rougeota, Ponthierc, and Deleuzec () have shown that neo‐ (XX) and super‐ (YY) male semen of Nile tilapia have similar motility to those of their XY males. In contrast, higher semen motility of XX males (73.1%) compared to XY males (48.4%) was reported in O. mykiss using the same semen harvest method (Kowalski et al, ).…”

Section: Discussionmentioning

confidence: 84%

Comparative gonad histology and semen quality of normal (XY) and neo‐males (XX) of Atlantic salmon (Salmo salar)

Castro

Patil

2019

Aquac Res

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With an overarching objective of improving the hatchery production of Atlantic salmon (Salmo salar) all‐female progeny, this study comparatively evaluated the reproductive parameters between normal (genotype XY) and neo‐males (genotype XX). Four normal (XY) and seven neo‐ (XX) males, from the same brood stock, distinguished by their ability to or lack of expressing semen, respectively, were comparatively evaluated. The left testicular lobe was used for histomorphometric analyses, while the right for semen collection and sperm quality analyses. Histomorphometric observations revealed that neo‐male testes are irregularly shaped, and have poorly formed seminiferous ducts, higher proportions of interstitial tissue and lower gonadosomatic index (p < .05). In addition, hypertrophied and cyst forming Sertoli cells were found in these individuals which collectively appear to form a physical barrier, precluding the semen collection by standard stripping techniques and reducing sperm quality. Particularly, semen motility (80.69 ± 2.4% and 57.2 ± 36.5% for XY and XX respectively) and duration of motility (99.31 ± 28.03 s and 66.84 ± 23.83 s for XY and XX respectively) of neo‐males were most compromised (p < .05). Interestingly, the TUNEL assay indicated no signs of apoptotic tissue suggesting that the histological differences may relate to delayed physiological/sexual maturity of neo‐males.

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“…Sperm concentration was assessed by conventional hemocytometry using a phasecontrast microscope (400 × magnification) according to a previous study (Gennotte et al, 2012). In each case, sperm samples were diluted with sperm preservative fluid (63 mM NaCl, 19 mM KCl, 1.3 mM CaCl 2 , 4.7 mM MgSO 4 ·7H 2 O, 2.5 mM NaHCO 3 , pH 7.4) to a concentration of 6 × 10 8 sperms/mL.…”

Section: Evaluation Of Sperm Motility and In Vitro Fertilization Effimentioning

confidence: 99%

Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish

Jin

Xie

et al. 2017

Aquaculture

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Sperm quality analysis in XX, XY and YY males of the Nile tilapia (Oreochromis niloticus)

Cited by 24 publications

References 47 publications

The Reversible Sex of Gonochoristic Fish: Insights and Consequences

The Reversible Sex of Gonochoristic Fish: Insights and Consequences

Comparative gonad histology and semen quality of normal (XY) and neo‐males (XX) of Atlantic salmon (Salmo salar)

Beneficial effect and potential molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish

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