Sperm nuclear histone H2B: correlation with sperm DNA denaturation and DNA stainability

Zini, Armand; Zhang, Xiaoyang; Gabriel, Maria San

doi:10.1111/j.1745-7262.2008.00415.x

Cited by 30 publications

(19 citation statements)

References 37 publications

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“…). Similarly, both focal and diffuse immunofluorescence signals for histone H2B were observed in human sperm by Zini et al []. In our investigation, we found strips or disk‐like localization patterns for modified histones and high concentrations of protamines very close to the periphery of the sperm nucleus (Figs.…”

Section: Discussionsupporting

confidence: 88%

Post‐Translational Modifications of Histones in Human Sperm

Krejčí

Stixová

Pagáčová

et al. 2015

J of Cellular Biochemistry

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We examined the levels and distribution of post-translationally modified histones and protamines in human sperm. Using western blot immunoassay, immunofluorescence, mass spectrometry (MS), and FLIM-FRET approaches, we analyzed the status of histone modifications and the protamine P2. Among individual samples, we observed variability in the levels of H3K9me1, H3K9me2, H3K27me3, H3K36me3, and H3K79me1, but the level of acetylated (ac) histones H4 was relatively stable in the sperm head fractions, as demonstrated by western blot analysis. Sperm heads with lower levels of P2 exhibited lower levels of H3K9ac, H3K9me1, H3K27me3, H3K36me3, and H3K79me1. A very strong correlation was observed between the levels of P2 and H3K9me2. FLIM-FRET analysis additionally revealed that acetylated histones H4 are not only parts of sperm chromatin but also appear in a non-integrated form. Intriguingly, H4ac and H3K27me3 were detected in sperm tail fractions via western blot analysis. An appearance of specific histone H3 and H4 acetylation and H3 methylation in sperm tail fractions was also confirmed by both LC-MS/MS and MALDI-TOF MS analysis. Taken together, these data indicate that particular post-translational modifications of histones are uniquely distributed in human sperm, and this distribution varies among individuals and among the sperm of a single individual.

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Section: Discussionsupporting

confidence: 88%

Post‐Translational Modifications of Histones in Human Sperm

Krejčí

Stixová

Pagáčová

et al. 2015

J of Cellular Biochemistry

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“…During mammalian spermiogenesis, packaging of the paternal chromosome complement into the highly condensed transport form is not a well-understood process, despite its central role for the survival of the species and despite increasing evidence for associations between low sperm chromatin quality and infertility, increased miscarriage rates, and likely also embryonic failure [5,[54][55][56]. One of the hallmarks of adequate sperm chromatin condensation is the removal of most of the histones and their replacement first with transition proteins and then protamines (Fig.…”

Section: Discussionmentioning

confidence: 98%

Poly(ADP-ribose) Metabolism Is Essential for Proper Nucleoprotein Exchange During Mouse Spermiogenesis

Meyer-Ficca

Ihara

Lonchar

et al. 2010

Biology of Reproduction

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Sperm chromatin is organized in a protamine-based, highly condensed form, which protects the paternal chromosome complement in transit, facilitates fertilization, and supports correct gene expression in the early embryo. Very few histones remain selectively associated with genes and defined regulatory sequences essential to embryonic development, while most of the genome becomes bound to protamine during spermiogenesis. Chromatin remodeling processes resulting in the dramatically different nuclear structure of sperm are poorly understood. This study shows that perturbation of poly(ADP-ribose) (PAR) metabolism, which is mediated by PAR polymerases and PAR glycohydrolase in response to naturally occurring endogenous DNA strand breaks during spermatogenesis, results in the abnormal retention of core histones and histone linker HIST1H1T (H1t) and H1-like linker protein HILS1 in mature sperm. Moreover, genetic or pharmacological alteration of PAR metabolism caused poor sperm chromatin quality and an abnormal nuclear structure in mice, thus reducing male fertility.

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“…Anomalies in the proportion of histones and protamines within a spermatozoon are correlated with DNA instability, and thus with vulnerability to damage. Higher histone levels have been observed in infertile individuals or those with reduced fertility that have certain anomalies: oligospermia -reduced numbers of mature spermatozoa, teratospermia -spermatozoa with abnormal structure, and asthenozoospermia -anomalous sperm motility (Zhang et al 2006, Zini et al 2008. These unique proteins and their possible role in early embryogenesis must be investigated in order to understand the epigenetic factors in male infertility.…”

Section: Discussionmentioning

confidence: 99%

Comparison of different chromatin staining techniques for bull sperm

Andraszek¹,

Banaszewska

Czubaszek³

et al. 2014

Arch. Anim. Breed.

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Morphological analysis of semen is a very important step in fertility assessment, but many semen defects are not detectable at the morphological level. These include pathological changes in sperm chromatin structure. During mammalian spermiogenesis, histone proteins associated with DNA structure are replaced by specific protamines, with which chromatin does not form nucleosomal complexes. In the fully developed, mature sperm, the histones are replaced with protamines. Disruptions of nucleoprotein structure can be treated as possible indicators of the biological value of spermatozoa. The experimental material consisted of sperm from one-and-a-half-year-old bulls, isolated post mortem from the tail of the epididymis. The smears were stained with silver nitrate (AgNO 3 ), acridine orange (AO), aniline blue (AB) and chromomycin A3 (CMA3). Sperm dimensions largely depend on individual variability among the bulls. In most cases, differences in sperm dimensions were identified between individuals, which was confirmed in the statistics. Sperm with elevated, abnormal histone levels were proportionally quite scarce (1.4 %). Studies of nuclear proteins in the context of infertility demonstrate the important influence of normal chromatin structure on sperm functions.

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Sperm nuclear histone H2B: correlation with sperm DNA denaturation and DNA stainability

Abstract: The data demonstrate that infertile men have a higher proportion of spermatozoa with diffuse histone H2B than do fertile men and suggest that sperm DNA damage might, at least in part, be due to abnormally high histone H2B levels.

Cited by 30 publications

References 37 publications

Post‐Translational Modifications of Histones in Human Sperm

Post‐Translational Modifications of Histones in Human Sperm

Poly(ADP-ribose) Metabolism Is Essential for Proper Nucleoprotein Exchange During Mouse Spermiogenesis

Comparison of different chromatin staining techniques for bull sperm

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