Sperm kinetics of Egyptian buffalo bulls (Bubalus bubalis) affected by the red laser postfreezing

Dessouki, Sherif Mohamed; Ahmed, Dalia Abd-El Rahman; Fayed, Ayat Kassem

doi:10.5455/javar.2022.i607

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…It is reported that buffalo semen is more sensitive to freezing and thawing than bull semen, resulting in lower fertilization potential (Dessouki et al, 2022). The value of sperm motility after thawing in this study follows SNI for frozen buffalo semen No.…”

Section: Discussionmentioning

confidence: 53%

Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen

Riwu,

Arifiantini,

Karja

2023

Trop. Anim. Sci. J.

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The objective of this study was to compare the quality of frozen semen from Toraya buffalo with different lecithins from different commercial diluents. Fresh semen from two 6-9 years old Toraya buffaloes were collected once a week in the morning through an artificial vagina. Fresh semen was examined macroscopically and microscopically. Semen with more than 70% sperm motility was divided into three tubes, each diluted with Andromed (soy lecithin), Steridyl (egg yolk lecithin), and Bovifree (synthetic lecithin) diluents at a concentration of 100 x10 6 mL -1 motile sperm. The diluted sperm were placed in 0.25 mL straws and allowed to equilibrate for 4 hours. The sperm was frozen above liquid nitrogen favour for 15 minutes and stored in liquid nitrogen containers for further evaluation. The quality of frozen semen was assessed 24 hours after freezing. The parameters tested were motility, viability, abnormalities, plasma membrane integrity, and sperm recovery rate (RR). The results showed no significant differences in the values of motility, viability, abnormalities, plasma membrane integrity, and sperm RR in the three diluents used. Sperm motility values ranged from 45.93% to 47.09% after freezing. Sperm viability ranged from 56.21% to 60.27%. The values of membrane integrity of the three diluents used ranged from 57.73% to 61.36%. The values of sperm abnormalities after freezing and thawing ranged from 2.74% to 3.18%. In conclusion, three commercial diluents containing animal, vegetable, and synthetic lecithin bases can be used as diluents for freezing Toraya buffalo semen with similar results.

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Section: Discussionmentioning

confidence: 53%

Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen

Riwu,

Arifiantini,

Karja

2023

Trop. Anim. Sci. J.

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“…According to the method of Dessouki et al [ 35 ], the software CASA sperm analyzer (Sperm Vision; Ref: 12520/5000; Minitube Hauptstrae 41. 84184 Tiefenbach, Germany) was applied to provide more information on the live images of various sperm motion characteristics captured by the Olympus computer-assisted microscope.…”

Section: Methodsmentioning

confidence: 99%

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Khalil,

Hassan,

El-Harairy

et al. 2023

Animals

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Thymoquinone nanoparticles (TQNPs) are broadly utilized in numerous pharmaceutical applications. In the present study, we tested the effects of TQNP supplementation on sperm quality and kinematics, acrosome exocytosis, oxidative biomarkers, apoptosis-like and morphological changes of frozen–thawed buffalo sperm, as well as the fertilizing capacity. Semen was collected from buffalo bulls, diluted (1:10; semen/extender), and divided into five aliquots comprising various concentrations of TQNP 0 (CON), 12.5 (TQNP12.5), 25 (TQNP25), 37.5 (TQNP37.5), and 50 (TQNP50) µg/mL, and then cryopreserved and stored in liquid nitrogen (−196 °C). The results revealed that TQNPs (25 to 50 µg/mL) provided the most optimal results in terms of membrane integrity (p < 0.001) and progressive motility (p < 0.01). In contrast, TQNP50 resulted in a greater post-thawed sperm viability (p = 0.02) compared with other groups. The addition of TQNPs to the extender had no discernible effects on sperm morphology measures. Sperm kinematic motion was significantly improved in the TQNP50 group compared to the control group (p < 0.01). TQNPs effectively reduced the content of H2O2 and MDA levels and improved the total antioxidant capacity of post-thawed extended semen (p < 0.01). The addition of TQNP significantly increased the number of intact acrosomes (p < 0.0001) and decreased the number of exocytosed acrosomes (p < 0.0001). A significant reduction in apoptosis-like changes was observed in TQNP groups. The non-return rates of buffalo cows inseminated with TQNP50-treated spermatozoa were higher than those in the control group (p < 0.05; 88% vs. 72%). These findings suggested that the freezing extender supplemented with TQNPs could effectively enhance the cryotolerance and fertility of buffalo sperm.

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“…However, Van de Hoek et al (2022) highlighted that CASA analysis provides essential information for semen quality assessment. Furthermore, using CASA, Dessouki et al (2022) examined buffalo semen and discovered variations in sperm motility and velocity parameters between fresh and frozen-thawed samples.…”

Section: Kinematics Of Fresh and Frozen Ram Spermmentioning

confidence: 99%

Quality and Protein Profiles in Local Indonesian Ram Sperm Before and After Cryopreservation

2024

Int J Vet Sci

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Sperm quality plays a crucial role in determining the success of fertilization. This study aimed to assess the quality of both fresh and frozen ram semen, as well as examine changes in semen protein content. Four local rams, aged 3-4 years, were selected for the study. Semen collection was conducted in the morning using an artificial vagina. The collected semen was divided into two parts: one for analyzing fresh semen and the other for freezing. The freezing process involved using a diluent consisting of Tris hydroxy amino methane, citric acid, egg yolk fructose, 20% egg yolk, and glycerol. Both fresh and frozen semen samples underwent analysis using the same method, including examination of concentration, motility, viability, and various kinematic motility parameters, such as VAP, VCL, VSL, LIN, and STR, with the assistance of CASA (AndroVision®). Following collection, both samples were centrifuged at 4500rpm for 10min, and the supernatant and precipitate were stored at -40°C until analysis. Protein concentration was determined using the Bradford kit, and semen protein profiles were evaluated through SDS-PAGE and Coomassie Blue staining. The study revealed a significant decrease in semen quality following cryopreservation (P<0.05). Protein analysis results indicated that fresh semen exhibited ten specific proteins, whereas frozen semen expressed six specific proteins. These findings suggested a correlation between protein content and sperm quality, with the cryopreservation process altering the composition of proteins in sperm.

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Sperm kinetics of Egyptian buffalo bulls (Bubalus bubalis) affected by the red laser postfreezing

Cited by 7 publications

References 19 publications

Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen

Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Quality and Protein Profiles in Local Indonesian Ram Sperm Before and After Cryopreservation

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