Sperm DNA damage diagnostics: when and why

Dada, Rima

doi:10.21037/tau.2017.05.26

Cited by 21 publications

(14 citation statements)

References 21 publications

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“…Esteves, Prudencio, et al, 2014;Mitchell et al, 2006;Strassburger et al, 2000). The DNA oxidative damage may cause mutations or dysregulate methylation processes and genetic pathways critical for embryo development and implantation (Aitken, 2017a;Dada, 2017;Feng et al, 2003).…”

Section: In Vitro Fertilisation/intracytoplasmic Sperm Injectionmentioning

confidence: 99%

“…When DNA repair is complete, both the copy′s fidelity and the orthodox gene order housed in the chromosome allow the morula and blastocyst stages to be reached. In this case, the paternal genome would be normally regulated and expressed, and a success- (Aitken, 2017a;Champroux et al, 2016;Dada, 2017;Jin et al, 2015). Moreover, the SDF type (SS-DBs or DS-DBs) and quantity might also differentially affect embryo development.…”

Section: In Vitro Fertilisation/intracytoplasmic Sperm Injectionmentioning

confidence: 99%

See 1 more Smart Citation

Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations

et al. 2020

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Section: In Vitro Fertilisation/intracytoplasmic Sperm Injectionmentioning

confidence: 99%

Section: In Vitro Fertilisation/intracytoplasmic Sperm Injectionmentioning

confidence: 99%

Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations

et al. 2020

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“…Sperm DNA damage is a major cause of defective sperm function and infertility 37 and is linked to decreased pregnancy rates of natural fertilization, intrauterine insemination and in vitro fertilization 38 . Assessment of sperm DNA damage is a better independent predictor of male fertility than traditional semen analysis 5 .…”

Section: Discussionmentioning

confidence: 99%

Studying the mechanism of sperm DNA damage caused by folate deficiency

Wang

Peng

Yuan

et al. 2021

J Cellular Molecular Medi

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Sperm DNA injury is one of the common causes of male infertility. Folic acid deficiency would increase the methylation level of the important genes, including those involved in DNA double‐strand break (DSB) repair pathway. In the early stages, we analysed the correlation between seminal plasma folic acid concentration and semen parameters in 157 infertility patients and 91 sperm donor volunteers, and found that there was a significant negative correlation between seminal folic acid concentration and sperm DNA Fragmentation Index (DFI; r = −0.495, p < 0.01). Then through reduced representation bisulphite sequencing, global DNA methylation of sperm of patients in the low folic acid group and the high folic acid group was analysed, it was found that the methylation level in Rad54 promoter region increased in the folic acid deficiency group compared with the normal folic acid group. Meanwhile, the results of animal model and spermatocyte line (GC‐2) also found that folic acid deficiency can increase the methylation level in Rad54 promoter region, increased sperm DFI in mice, increased the expression of γ‐H2AX, that is, DNA injury marker protein, and increased sensitivity of GC‐2 to external damage and stimulation. The study indicates that the expression of Rad54 is downregulated by folic acid deficiency via DNA methylation. This may be one of the mechanisms of sperm DNA damage caused by folate deficiency.

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“…Sperm DNA damage is considered to be a major cause of defective sperm function and infertility (Dada, ). It is linked to decreased pregnancy rates of natural fertilisation, intrauterine insemination and in vitro fertilisation (Zini & Sigman, ).…”

Section: Introductionmentioning

confidence: 99%

Methylation levels ofIGF2andKCNQ1in spermatozoa from infertile men are associated with sperm DNA damage

Pan

et al. 2019

Andrologia

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Abnormal imprinted genes methylation in spermatozoa has been shown to be associated with subfertility. However, the relationship between sperm DNA damage and specific imprinted genes methylation remains unclear. In this study, DNA methylation levels were determined at seven imprinted genes loci (H19, INS‐IGF2, KCNQ1, MEG3, MEST, PEG3 and SNRPN) in 66 semen samples using the MSRE‐qPCR method. The semen samples were divided into two groups according to the threshold value (25%) of DNA fragmentation index (DFI). We found that the mean methylation level at IGF2 (cg17037101) in the group with DFI ≥ 25% was lower than that in the group with DFI < 25% (13.7 ± 3% vs. 31.5 ± 5.3%, p = 0.0053). However, the methylation levels of other CpGs did not differ from the imprinted genes. Correlation analysis of DFI with the methylation levels of imprinted genes demonstrated that the IGF2 (cg17037101) methylation level was negatively correlated with sperm DFI (r = −0.448, p = 0.0038), and the KCNQ1 (cg24932449) methylation level was positively correlated with sperm DFI (r = 0.354, p = 0.0273). Our results suggest that the aberrant methylation of IGF2 and KCNQ1 genes may be associated with sperm DNA damage.

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Sperm DNA damage diagnostics: when and why

Cited by 21 publications

References 21 publications

Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations

Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations

Studying the mechanism of sperm DNA damage caused by folate deficiency

Methylation levels ofIGF2andKCNQ1in spermatozoa from infertile men are associated with sperm DNA damage

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