Sperm DNA and RNA abnormalities in fertile and oligoasthenoteratozoospermic smokers

Selit, I.; Basha, Mohamed Ahmed; Maraee, Alaa Hassan; El‐Naby, SobhyHasab; Nazeef, N.; El-Mehrath, R.; Mostafa, Taymour

doi:10.1111/j.1439-0272.2012.01305.x

Cited by 16 publications

(10 citation statements)

References 34 publications

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“…In support of our results, it has been shown that sperm DF increased in idiopathic infertile men (Saleh et al, 2003;Appasamy et al, 2007;Mahfouz et al, 2010), varicocele patients (Smith et al, 2006;Wang et al, 2012), infertile men with genitourinary infection (Gallegos et al, 2008), testicular hyperthermia (Ahmad et al, 2012), cigarette smoking (Selit et al, 2013), testicular cancer (O'Flaherty et al, 2008), vasectomy reversal (Smit et al, 2010) and cryptorchidism (Smith et al, 2007). Zhang et al (2009Zhang et al ( , 2010 measured the DF percentage in semen and showed that the amount of DF was higher in infertile groups compared with fertile groups.…”

Section: Discussionsupporting

confidence: 91%

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

et al. 2012

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The objective of this study was to investigate the association between the amount of superoxide anion, peroxynitrite as oxidative stress (OS) markers and total antioxidant capacity (TAC) with sperm DNA fragmentation in infertile men with abnormal semen parameters. Semen samples were obtained from 102 infertile couples and divided into groups with normal and abnormal semen parameters according to the World Health Organization (WHO). Peroxynitrite and superoxide anions were detected using spectrofluorometric assays combined with 2,7 dicholorofluorescein (DCF)-DA and 4-chloro-7-nitrobenzo-2-oxa -1, 3-diazole (NBD-CL). Colorimetric assay was used for evaluation of TAC, while DNA fragmentation was studied by using sperm chromatin dispersion test. Superoxide anion, peroxynitrite and DNA fragmentation were significantly higher in infertile couples with abnormal semen parameters as compared to infertile couples with normal semen (P < 0.01). TAC was significantly lower in infertile men with abnormal semen parameters (P < 0.01). There was also a significant positive correlation between OS markers with sperm DNA fragmentation (r = 0.59, P < 0.01 and r = 0.67, P < 0.01, respectively). We have found that imbalance between superoxide anion and peroxynitrite with antioxidant capacity in infertile men with abnormal sperm parameters is associated with higher sperm DNA fragmentation.

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Section: Discussionsupporting

confidence: 91%

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

et al. 2012

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“…Lately, Selit et al . () pointed to increased percentage of abnormal sperm DNA and RNA in fertile smokers compared with fertile nonsmokers and in OAT smokers compared with OAT nonsmokers. Increased percentage of severe, moderate sperm DNA and RNA damage was demonstrated in fertile heavy smokers compared with fertile light smokers and in OAT heavy smokers compared with OAT light smokers.…”

Section: Discussionmentioning

confidence: 93%

Smoking influence on sperm vitality, DNA fragmentation, reactive oxygen species and zinc in oligoasthenoteratozoospermic men with varicocele

et al. 2013

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This study aimed to assess the influence of smoking duration and intensity on sperm vitality, sperm DNA fragmentation, reactive oxygen species (ROS) and zinc (Zn) levels in oligoasthenoteratozoospermic (OAT) men with varicocele (Vx). A total of 246 men were investigated who were divided into OAT nonsmokers, OAT smokers, OAT nonsmokers and OAT smokers with Vx. They were subjected to history taking, clinical examination and semen analysis. In their semen, sperm hypo-osmotic swelling (HOS) test, sperm DNA fragmentation test, seminal ROS and seminal Zn were assessed. The results demonstrated significantly decreased HOS test, seminal Zn level and significantly increased sperm DNA fragmentation, seminal ROS levels in OAT smokers with Vx more than OAT smokers compared with OAT nonsmokers. Smoking intensity, smoking duration and Vx grade demonstrated significant negative correlations with sperm motility, HOS test percentage and significant positive correlations with sperm DNA fragmentation, seminal ROS level. It is concluded that smoking has a negative impact on sperm progressive motility, HOS test, seminal Zn and positive impact on sperm DNA fragmentation, semen ROS level that are exaggerated if Vx is associated being correlated with smoking intensity, smoking duration and Vx grade.

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“…In vitro sperm DNA damage inhibition activity of T. arjuna bark DNA damage inhibition activity of aqueous extract of T. arjuna bark was checked in cigarette smoke exposed irradiated infertile human semen DNA sample with control pBR322 plasmid DNA using simple modified non-enzymatic salting out DNA extraction method (Selit et al 2013). Three micro centrifuge tubes were added with a total of 1 ll of aliquots of smoke irradiated extracted sperm DNA sample.…”

Section: Seminal Plasma Zinc Analysismentioning

confidence: 99%

Human sperm DNA damage inhibition and antioxidant activity of T. arjuna bark: an in vitro study

Parameswari

Rao²,

Mano

et al. 2017

3 Biotech

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Complimentary or natural antioxidant type of alternative medicine is developed worldwide to treat male infertility. The aim of this study is to the extraction of T. arjuna bark and activity against human sperm DNA damage in asthenoteratospermic smoker's subjects-an in vitro study. All preliminary and antioxidant assays (DPPH, HO, and total antioxidant, reducing power activity) were done. T. arjuna bark metal analysis was done with AAS. On the other hand, patients were asked to fill a direct questionnaire about smoking history; 25 infertile smokers were identified as asthenoteratospermic; 34 fertile non-smokers (control) were assessed for semen parameters by CASA, seminal plasma Zinc analysis by AAS, DNA fragmentation by colorimetric method and semen genomic DNA damage inhibition by modified non-enzymatic salting out extraction method. Most of the antioxidants are highly present in the aqueous extract; meanwhile, the major content in this extract is zinc 16 µg/g (Ca = 0.5 µg/g; Se = 2.2 µg/g and Mg = 1.6 µg/g) along with FT-IR peaks which also confirmed the metal presence. The semen parameters in smokers that were noticed are low sperm count and morphological changes. Meanwhile, in the seminal plasma of smokers, zinc and DNA fragmentation results were positively correlated with sperm morphology (p < 0.001). Repaired DNA bands were noticed in the in vitro study of aqueous T. arjuna bark, in smokers' semen. T. arjuna bark will act as cryo protector as well as great zinc supplementary to maintain sperm motility and morphology in smokers.

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Sperm DNA and RNA abnormalities in fertile and oligoasthenoteratozoospermic smokers

Cited by 16 publications

References 34 publications

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

Smoking influence on sperm vitality, DNA fragmentation, reactive oxygen species and zinc in oligoasthenoteratozoospermic men with varicocele

Human sperm DNA damage inhibition and antioxidant activity of T. arjuna bark: an in vitro study

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