Sperm DNA and detection of DNA fragmentations in sperm

Küçük, Niyazi

doi:10.5152/tud.2018.49321

Cited by 22 publications

(7 citation statements)

References 56 publications

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“…Sperm DNA damage can evaluated using AO staining (Kucuk, 2018). This study indicated that all frozen semen in the various diluents had low sperm-DNA damage.…”

Section: Tasj-36996mentioning

confidence: 91%

The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents

Arif¹,

Maulana²,

Kaiin³

et al. 2022

Trop. Anim. Sci. J.

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Diluents are substances added to the semen to increase the semen volume, reduce sperm density, and maintain sperm viability. This study aimed to compare the effects of various semen diluents on the quality of frozen semen of Limousin bull. Three mature Limousin bulls were used as semen sources. The semen was collected using an artificial vagina and then evaluated for its quality. Sperms with motility less than 70% and abnormality greater than 20% were excluded from this study. After semen evaluation, each ejaculate was individually divided into four equal tubes and diluted with skimmed milk-egg yolk (SMEY), Tris-egg yolk (TEY), Tris-egg yolk-skimmed milk (TEYSM), or Andromed ® . Diluted semen was loaded into a mini straw and then equilibrated at 5 °C for 4 h. Following equilibration, the straws were frozen. The percentages of sperm motility, viability, intact membrane, and sperm DNA integrity were evaluated after freezing. Furthermore, malondialdehyde (MDA) and aspartate aminotransferase (AST) enzyme concentrations were assessed after thawing. The sperm motility with TEY, TEYSM, and Andromed ® did not differ significantly but was higher than with SMEY. Sperm viability, membrane intactness, and sperm DNA integrity did not differ among treatments. By contrast, the MDA levels and concentration of AST in the Andromed ® diluent semen showed the lowest values compared with SMEY semen (p<0.05) but did not differ from those of TEY and TEYSM semen. To conclude, TEY, TEYSM, and Andromed ® diluents performed equally well, and although they showed better results than SMEY, sperm diluted in SMEY are still suitable for artificial insemination.

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“…Sperm DNA damage can evaluated using AO staining (Kucuk, 2018). This study indicated that all frozen semen in the various diluents had low sperm-DNA damage.…”

Section: Tasj-36996mentioning

confidence: 91%

The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents

Arif¹,

Maulana²,

Kaiin³

et al. 2022

Trop. Anim. Sci. J.

View full text Add to dashboard Cite

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“…The TUNEL test generally consists of the fixation and permeabilization of sperm, the marking of DNA fragments, staining, and the evaluation of the stages of sperm. In the evaluation process, the sperm can be assessed subjectively under light and fluorescence microscopy or by flow cytometry [ 28 ]. The TUNEL assay can also be used to identify apoptotic cells in the tissues during histological examinations.…”

Section: Methodsmentioning

confidence: 99%

Protective effects of curcumin on chromatin quality, sperm parameters, and apoptosis following testicular torsion-detorsion in mice

Shahedi

Talebi

Mirjalili

et al. 2021

Clin Exp Reprod Med

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Objective: The chief outcome of testicular torsion in clinical and experimental contexts is testicular ischemia. Curcumin, a compound with anti-inflammatory and antioxidant properties, has fascinated researchers and clinicians for its promise in the treatment of fertility diseases. Methods: Thirty-five fully grown male mice were randomly classified into five groups: control, sham, testicular torsion, treatment group 1 (testicular torsion+short-term curcumin), and treatment group 2 (testicular torsion+long-term curcumin). Thirty-five days later, spermatozoa from the right cauda epididymis were analyzed with regard to count and motility. Toluidine blue (TB), aniline blue (AB), and chromomycin A3 (CMA3) staining assays were used to evaluate the sperm chromatin integrity. In addition, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) test was used to assess apoptosis.Result: Treatment group 1 exhibited a remarkably elevated sperm count compared to the testicular torsion group. Additionally, notably lower sperm motility was found in the testicular torsion group compared to the control, treatment 1, and treatment 2 groups. Staining (CMA3, AB, and TB) and the TUNEL test indicated significantly greater testicular torsion in the torsion group compared to the control group (p<0.05). The data also revealed notably lower results of all sperm chromatin assays and lower apoptosis in both treatment groups relative to the testicular torsion group (p<0.05). Significantly elevated (p<0.05) AB and TB results were noted in treatment group 1 compared to treatment group 2.Conclusion: Curcumin can compensate for the harmful effects of testicular ischemia and improve sperm chromatin quality in mice.

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“…It is important to correlate routinely evaluated sperm parameters at the moment of thawing with sperm viability and sperm DNA fragmentation evaluations, which together are able to correctly qualify an ideal sperm for fertilization. In the comet assay, sperm cells are placed on agarose gel using glass slides and, after cell discharge and DNA denaturation, alkaline electrophoresis is performed (Beirão, Cabrita, Soares, Herráez, & Dinis, 2008;Küçük, 2018;Lee & Steinert, 2003). The technique is based on different electrophoretic DNA patterns obtained from DNA fragments, where cells (DNA spermatozoa) with DNA archived in a comet-like tail structure are observed, larger when the DNA structure damage is greater (Gallego & Asturiano, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

Paula

Murgas

Castro

et al. 2019

Reprod Domestic Animals

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This study aims to investigate the effect of different cooling rates on the semen cryopreservation of curimba (Prochilodus lineatus). Nineteen ejaculates were obtained from adults males and cryopreserved at 15°C/min (CR15), 30°C/min (CR30) (controlled temperature inside and outside straw, speed was stable during freezing) and direct freezing in liquid nitrogen vapour (~35.6°C/min) (CRNV). The straws were thawed and seminal parameters evaluated. DNA fragmentation through the comet assay was assessed. A fresh sperm sample was not frozen and used for analyses. Data were submitted to an analysis of variance (ANOVA), and means were compared by Scott–Knott test (p < 0.05) using the R Software. Mean motility percentage was 100%, and motility duration was 39.5 ± 5.7 s for the fresh sperm (subjective analysis); 58.9 ± 8.0% and 24.5 ± 5.7 s for CR15; 64.8 ± 4.8% and 26.5 ± 7.1 s for CR30; and 50.1 ± 16% and 25.7 ± 4.7 s for CRNV, respectively. Motility percentages were higher and equal between CR15 and CR30 compared to CRNV (p < 0.05). Some sperm motion kinetics, namely average path velocity (VAP) and straight line velocity (VAS), were higher for CR30 (p < 0.05), while curvilinear velocity (VCL) and velocity progression (PRO) were lower for CRNV (p < 0.05). Straightness (STR) and wobble (WOB) were the same among treatments (p > 0.05). Sperm morphology results indicated higher means for total morphological sperm alterations in CRNV. All cooling rates caused sperm DNA fragmentation, although CR30 provided a less harmful effect. This is the first report for cryopreserved P. lineatus sperm preserved under different controlled cooling rates. The cooling rate of 30°C/min is indicated for the cryopreservation of this fish sperm as it led to the lowest detrimental spermatozoa effects.

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Sperm DNA and detection of DNA fragmentations in sperm

Cited by 22 publications

References 56 publications

The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents

The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents

Protective effects of curcumin on chromatin quality, sperm parameters, and apoptosis following testicular torsion-detorsion in mice

Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

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