2015
DOI: 10.1017/s0967199415000635
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Sperm dilution ratio affects post-thaw motility rate and velocity of Prochilodus lineatus (Characiformes) sperm

Abstract: There is a lack of standardization in sperm cryopreservation of aquatic organisms and, thus, a necessity of more accurate investigations in all steps of this process. In this study, the effects of sperm dilution ratio on post-thaw sperm quality of Prochilodus lineatus were evaluated. Sperm was diluted in a standard freezing medium (glucose and methyl glycol) at four different ratios (sperm to final volume = 1:5, 1:10, 1:50 or 1:100), frozen in a nitrogen vapour vessel at -170°C and then stored in liquid nitrog… Show more

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Cited by 7 publications
(2 citation statements)
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“…Suquet et al (2016) reported 75% lower velocity in frozen/thawed spermatozoa of the great scallop compared to fresh. Linhart et al (2005) (Figueroa et al 2016), 97 µm s -1 to 134 µm s -1 in Prochilodus lineatus (Viveiros and Leal 2016;Viveiros et al 2017), 57 µm s -1 in rainbow trout Oncorhynchus mykiss (Suquet et al 2012b) and µm s -1 in Siberian sturgeon Acipenser baerii spermatozoa (Sarosiek et al 2014). VSL of sea urchin spermatozoa was shown to be 170-240 µm sec -1 in Hemicentrotus pulcherrimus (Hiramoto and Baba 1978) and 150-200 µm sec -1 in Paracentrotus lividus (Fabbrocini and D'Adamo 2017).…”
Section: Discussionmentioning
confidence: 97%
“…Suquet et al (2016) reported 75% lower velocity in frozen/thawed spermatozoa of the great scallop compared to fresh. Linhart et al (2005) (Figueroa et al 2016), 97 µm s -1 to 134 µm s -1 in Prochilodus lineatus (Viveiros and Leal 2016;Viveiros et al 2017), 57 µm s -1 in rainbow trout Oncorhynchus mykiss (Suquet et al 2012b) and µm s -1 in Siberian sturgeon Acipenser baerii spermatozoa (Sarosiek et al 2014). VSL of sea urchin spermatozoa was shown to be 170-240 µm sec -1 in Hemicentrotus pulcherrimus (Hiramoto and Baba 1978) and 150-200 µm sec -1 in Paracentrotus lividus (Fabbrocini and D'Adamo 2017).…”
Section: Discussionmentioning
confidence: 97%
“…Conversely, a higher DR might cause more glycerol to penetrate sperm cells, as a higher glycerol ratio per sperm cell is available, and consequently sperm quality would be diminished. Higher DRs may also reduce sperm post-thaw quality through dilution of several proteins or antioxidants present in seminal plasma that protect sperm against freezing-thawing (Viveiros and Leal, 2016).…”
Section: Discussionmentioning
confidence: 99%