Sperm cryopreservation of the critically endangered olive barb (Sarpunti) Puntius sarana (Hamilton, 1822)

Nahiduzzaman, Md.; Hassan, Mahbubul; Khanam, U. Habiba; Mamun, Shaheed Nasrullah Al; Hossain, Mostafa Ali Reza; Tiersch, Terrence R.

doi:10.1016/j.cryobiol.2010.12.004

Cited by 24 publications

(14 citation statements)

References 40 publications

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“…Similar results with 10% concentration were reported from many species by Withler and Lim (1982); Shirohara et al (1982) and Chao et al (1975). Incubation of Olive barb (Puntius sarana) sperm with 5% DMSO and methanol showed longer motility and 15% concentration seemed toxic to sperm (Nahiduzzaman et al, 2011). DMSO and methanol at 5 and 10% concentrations produced better motility of sperm of Ompok pabda and Nandus nandus during 5 and 10 min incubation but 15% was toxic to sperm (Sarder et al, 2012 and2013).…”

Section: Discussionsupporting

confidence: 76%

“…Rao (1989) argued that DMSO along with Alsever's solution might play a positive role in preservation by easily penetrating into the cellular membrane and bringing a balance between the intra and extra-cellular fluid concentrations (Ciereszko et al, 1993). In contrast, methanol yielded comparatively poor post-thaw motility; however, it was suitable for cryopreservation of sperm of zebra fish (Yang et al, 2007) and Olive barb (Nahiduzzaman et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Viveiros et al (2001) also investigated the optimum cooling rate of sperm of African catfish (Clarias gariepinus) and temperature falling at -5 to -10 o C/min was found most suitable. Protocols for cryopreservation of sperm of endangered Ompok pabda (Sarder et al, 2013); Nandus nandus (Sarder et al, 2012) and minor carps, Olive barb (Nahiduzzaman et al, 2011) have been developed in Bangladesh. Though M. cavasius is becoming endangered, it has not been a subject to cryopreservation anywhere in the world.…”

Section: Mystus Cavasiusmentioning

confidence: 99%

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Development of a sperm cryopreservation protocol of Gangetic Mystus (Mystus cavasius)

et al. 2017

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Cryopreservation is considered as one of the most useful techniques for long-term preservation of genetic material specially sperm of fish. This study focused on the development of a sperm cryopreservation protocol for indigenous near threatened gulsha (Mystus cavasius) and a number of experiments were conducted for the purpose. To collect milt, male gulsha were sacrificed and milt was suspended in extenders. Different concentrations of NaCl were used to evaluate the activation of sperm motility and it decreased as the concentration of the extending media increased, therefore, motility was completely inhibited at 0.8% and 1.2% NaCl solution when sperm suspended in Kurokura-2 and Alsevers solution, respectively. The toxicity of cryoprotectants to sperm were evaluated using two cryoprotectants, dimethyl sulfoxide (DMSO) and methanol along with the extenders, Alsevers solution and Kurokura-2 solution. DMSO and methanol with 5% and 10% concentrations produced significantly higher motility during 5 and 10 min incubation and their 15% concentration found toxic to sperm. Alsevers solution with 10% DMSO produced best equilibration (83.75±2.39%) as well as post-thaw motility (67.5±3.23%) while Kurokura-2 solution with DMSO produced similar equilibration motility (81.25±2.39%) but the post-thaw motility (50.0±6.12%) was significantly much lower than that of Alsevers solution. Sperm preserved with Alsevers solution plus DMSO produced highest fertilization, 72.5±7.5% and hatching, 56.8±5.6% while fresh sperm yielded 85.0±5.0% and 74.8±3.6% fertilization and hatching, respectively. The protocols that have been developed can be used for conservation of genetic materials of M. cavasius and other endangered fish species and new generations of them can be propagated using the cryopreserved sperm.Progressive Agriculture 27 (4): 517-529, 2016

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Mystus Cavasiusmentioning

confidence: 99%

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Development of a sperm cryopreservation protocol of Gangetic Mystus (Mystus cavasius)

et al. 2017

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“…Although various cryoprotectants have been used successfully to freeze cyprinid sperm, few studies have been conducted on cryopreservation of Barbodes sperm. Nahiduzzaman et al (2011) reported the highest motility of thawed sperm of Puntius sarana from a treatment using 10% dimethylsulfoxide (DMSO), 8-10 min of equilibration time and one-step freezing rate at 10°C min À1 from 5 to À80°C. Little information is available on the use of common cryoprotectants such as propylene glycol, sucrose and methanol in freezing B. gonionotus sperm.…”

Section: Introductionmentioning

confidence: 99%

“…Critical steps of cryopreservation technology are associated with chemical optimization of extender and cryoprotectant as well as equilibration time and freezing and thawing rate. Several extenders have been used to cryopreserve freshwater fish sperm, including Alsever's solution (Nahiduzzaman, Hassan, Khanam, Mamuna, Hossain & Tiersch 2011), calcium-free Hank's balanced salt solution (Ca-F HBSS; Kwantong & Bart 2003) and buffered sperm motility-inhibiting saline solution (Lahnsteiner, Berger, Horvath, Urbanyi & Weismann 2000). Although various cryoprotectants have been used successfully to freeze cyprinid sperm, few studies have been conducted on cryopreservation of Barbodes sperm.…”

Section: Introductionmentioning

confidence: 99%

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

et al. 2014

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Effectiveness and efficiency of frozen sperm on fertilization and hatching success of eggs from silver barb was examined in relation to cryoprotectants, freezing rate and storage period. Sperm was diluted in calcium-free Hank's balanced salt solution, equilibrated with dimethylsulphoxide (DMSO), propylene glycol, sucrose or methanol at 5%, 10%, 15% or 20% final concentrations, and frozen in 250-lL straws using a one-step freezing procedure (1, 5 and 8°C min À1 from 25 to À40°C). Highest post-thaw sperm motility was found from a treatment using 10% DMSO and 5°C min À1 (82.2 AE 2.1%), similar to that of 10% DMSO and 8°C min À1 (87.8 AE 3.2%). Post-thaw motility of sperm frozen at 5 or 8°C min À1 was significantly higher than 1°C min À1 . Relative sperm motility declined significantly after 10 months of cryostorage while viability did not change during a 12-month cryostorage. Average fertilization rates of sperm after 1 and 4 months of storage were 64.5 AE 4.6% and 61.3 AE 3.4%, respectively, similar to those of fresh sperm (69.6-72.3%). Hatching rates of cryopreserved sperm (45.4-51.2%) were similar to those of fresh sperm (51.8-57.8%). This study developed suitable methods for cryopreservation of silver barb sperm that can be used to facilitate hatchery operation.

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Potential of Fish Gamete Cryopreservation in Conservation Programs in Bangladesh

Sarder

2020

Cryopreservation of Fish Gametes

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Sperm cryopreservation of the critically endangered olive barb (Sarpunti) Puntius sarana (Hamilton, 1822)

Cited by 24 publications

References 40 publications

Development of a sperm cryopreservation protocol of Gangetic Mystus (Mystus cavasius)

Development of a sperm cryopreservation protocol of Gangetic Mystus (Mystus cavasius)

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

Potential of Fish Gamete Cryopreservation in Conservation Programs in Bangladesh

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