Peste des petits ruminants (PPR), an economically important morbillivirus infection of sheep and goats, is widely distributed in sub-Saharan Africa, Middle East and western and southern Asia including Bangladesh. A small flock of Black Bengal goats contracted PPR following introduction of new animals. A pathological investigation was conducted on the outbreak, the viral RNA corresponding to F gene was detected by RT-PCR and the virus was isolated in Vero cells. Out of 37 goats 19 (51 %) developed clinical disease, of which 5 (13.5 %) died. Goats under one year of age had highest morbidity and mortality with typical signs and lesions of PPR. Viral RNA could be detected in mesenteric and bronchial lymph node tissues. Typical cytopathic effects (CPE) in Vero cells following inoculation of lymph node tissue homogenate were visible at the third passage. However, the replication of virus in cell culture was detected by RT-PCR at the first and second passage in the absence of visible CPE. RT-PCR appears to be a very useful and sensitive tool not only for the detection of PPR virus in clinical samples but also for monitoring the growth of virus in cell culture following inoculation. (Bangl. vet.
A cell-culture-adapted reverse genetics strain of very virulent infectious bursal disease virus (IBDV) of chickens, designated as BD-3tcC, having four amino acid substitutions (Gln253His, Asp279Asn, Ala284Thr and Ser330Arg) in the capsid protein VP2 was tested for its genetic stability during serial passage in chickens and chicken embryo fibroblast (CEF) cell culture. Results of in vitro and in vivo experiments demonstrated that all four introduced mutations in BD-3tcC remained stable during serial passage in CEF cell culture, but during passage in chickens, amino acid residues at position 253 and 284 reverted from histidine to glutamine and threonine to alanine, respectively. In a parallel experiment, the same substitutions also occurred in a conventionally attenuated vaccine strain D-78 on serial passage in chickens. However, no reversion or substitution took place at positions 279 and 330 during in vivo passage of the mutant virus BD-3tcC or vaccine virus D-78. The findings provide conclusive evidence that while IBDV requires histidine and threonine at positions 253 and 284 for cell culture adaptation, glutamine and alanine at these positions are selected preferentially during in vivo replication.
The experiment was carried out at the research farm of Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh during December 2015 to January 2016 to evaluate the effects of cow dung (CD), poultry manure (PM) and mustard oil cake (MOC) along with NPK fertilizer amendments on the growth and yield performances of red amaranth in silty loam soil. The treatments were (i) NPK (control), (ii) NPK + CD, (iii) NPK + PM and (iv) NPK + MOC. All three types of manures significantly increased the plant height and numbers of leaves plant-1, thereby fresh weight, yield compared with the control (NPK). The best results in terms of vegetable fresh yield were obtained in the following order: NPK > CD > PM > MOC. Application of MOC is not beneficial due to 30 times higher price than CD and PM. PM added NPK fertilizer combination showed higher vegetable yield (10.87 t ha-1) than CD added NPK fertilizer combination (10.17 t ha-1). PM added fertilizer combination also had almost equal investment and cost-benefit ratio in comparison with CD. The lowest marginal benefit-cost ratio (1.09) was observed in MOC added NPK fertilizer combination though it showed higher vegetable yield (11.08 t ha-1). The farmers’ can be recommended to follow NPK + CD fertilizer combination. Manures applied post soil analysis showed improves the soil physiochemical properties. Res. Agric., Livest. Fish.6(2): 263-269, August 2019
This study was carried out with a view to evaluating the effect of foliar application of urea on BRRI dhan29. The experiment was laid out in a RCBD design with seven treatments (T 1 : Full dose of prilled urea @ 140 Kg N ha -1 ) (control), T 2 (50% prilled urea + 5 foliar spray @ 0.5% N solution @ 5.5 kg N spray -1 ),T 3 (70% prilled urea + 3 foliar spray @ 0.5% N solution @ 5.5 kg N spray -1 ), T 4 (50% prilled urea + 3 foliar spray @ 1.0% N solution @ 11 kg N spray -1 ), T 5 (50% prilled urea + 5 foliar spray @ 0.5% N solution @ 5.5 kg N spray -1 + 5 t ha -1 cow dung), T 6 (70% prilled urea + 3 foliar spray @ 0.5% N solution @ 5.5 kg N spray -1 + 5 t ha -1 cow dung) and T 7 (50% prilled urea + 3 foliar spray @ 1.0% N solution @ 11 kg N spray -1 + 5 t ha -1 cow dung) and three replications. Soil and foliar application of nitrogen significantly influenced the growth and yield contributing characters like plant height, panicle length, and effective tillers hill -1 as well as the grain and straw yields of the crop. The treatment T 5 produced the highest grain yield (6.68 t ha -1 ) which was statistically similar to yield from T 7 , T 6 .Treatment T 2 ,T 3 and T 4 gave similar yield. The grain yields of these treatments were higher than those of T 1 treatment (soil application of urea only). The T 5 also produced the highest straw yield of the crop. The lowest grain yield of 4.72 t ha -1 and the lowest straw yield of 5.17 t ha -1 were recorded with T 1 (control). Manik et al.Reducing urea demand for rice crop through foliar application of ureaRes.
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