Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications

Gosálvez, Jaime; López‐Fernández, Carmen; Fernández, José Luis Fernández

doi:10.1007/978-1-4419-6857-9_10

Cited by 19 publications

(14 citation statements)

References 114 publications

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“…However, to the best of our knowledge, this is the first time that SDF analyses have been performed in the context of speciation research. Although SDF rates are usually correlated with other parameters traditionally used to measure sperm quality, infertile males with standard semen parameters show significantly higher rates of SDF (reviewed in Gosálvez et al ., ). These results suggest SDF rate as an important and independent estimator of male fertility.…”

Section: Discussionmentioning

confidence: 97%

“…However, post‐zygotic isolation can be estimated indirectly in wild by comparing partial fitness components between hybrids and parental types, with parameters such as the rate of sperm DNA fragmentation (SDF). In several species, including mammals, fishes and molluscs, SDF rates are negatively correlated with fertilization (Evenson et al ., , ; Morris et al ., ; Gwo et al ., ) and birth rates (Benchaib, ; Rybar et al ., ; Borini et al ., ; Gosálvez et al ., ,b) and positively correlated with abortion rates (Benchaib, ; Pérez‐Cerezales et al ., ). Offspring descending from sperm cells with fragmented DNA also show reduced adult viability and increased incidence of tumours (Fernández‐González et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Incipient post‐zygotic barrier in a model system of ecological speciation with gene flow

Sá-Pinto¹,

Martínez‐Fernández

López‐Fernández

et al. 2013

J of Evolutionary Biology

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The role of post-zygotic isolation in nonallopatric ecological speciation is still mostly unknown and information on the nature and strength of these barriers in well-known speciation models is essential for a deeper understanding of such processes. The Galician ecotypes of the marine snail Littorina saxatilis represent one of the best studied cases of nonallopatric ecological speciation. Here, we test the existence of incipient post-zygotic isolation by comparing the fertility of male hybrids with that of both pure forms [ridged and banded (RB) and smooth and unbanded (SU) ecotypes]. We analysed the degree of sperm DNA fragmentation (SDF) of individuals morphologically classified as RB, SU and hybrids, sampled from two locations. SDF analyses were chosen to study sperm quality because, in other animal species, SDF rates correlate with important parameters for speciation research, such as fertilization and abortion rates and viability of adult progeny. In the present work, hybrids showed significantly higher SDF rates than RB and SU males in one location and significantly higher variances in both locations. These results suggest the existence of an incipient post-zygotic barrier, the strength of which may vary across the Galician shore, and highlight the potential of SDF analyses for speciation research.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Incipient post‐zygotic barrier in a model system of ecological speciation with gene flow

Sá-Pinto¹,

Martínez‐Fernández

López‐Fernández

et al. 2013

J of Evolutionary Biology

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“…[53] In classical SCD test, while the sperm which produced halo were regarded as intact, the sperm which had no halo were regarded as DNA damaged. [53][54][55] Contrary to the classical version of SCD test, the sperm which produced halo are regarded as DNA damaged; the sperm which had no halo are regarded as intact in modified commercial version of the SCD test. [55][56][57][58] Comparison of tests used to detect DNA damage in spermatozoa In humans, SCSA, TUNEL and SCD tests have been found quite effective in detecting DNA damage in sperm contrary to AOT.…”

Section: Sperm Chromatin Dispersion Testmentioning

confidence: 99%

“…[53][54][55] Contrary to the classical version of SCD test, the sperm which produced halo are regarded as DNA damaged; the sperm which had no halo are regarded as intact in modified commercial version of the SCD test. [55][56][57][58] Comparison of tests used to detect DNA damage in spermatozoa In humans, SCSA, TUNEL and SCD tests have been found quite effective in detecting DNA damage in sperm contrary to AOT. [46] Although SCSA and AOT have same experimental principles; the reason of different results obtained from these tests might be a consequence of different evaluation procedures.…”

Section: Sperm Chromatin Dispersion Testmentioning

confidence: 99%

Sperm DNA and detection of DNA fragmentations in sperm

Küçük¹

2018

Turkish Journal of Urology

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The questionable effectiveness of routine sperm parameters in determining male factor infertility problems and increasing the success rates of assisted reproductive techniques have led to the investigation of more detailed sperm parameters that could affect the male fertility and reproduction. Thus, the effects of different sperm parameters such as sperm DNA integrity was started to be investigated thanks to the previously described methods such as single cell gel electrophoresis (COMET) assay, sperm chromatin structure assay (SCSA), acridine orange test (AOT), terminal deoxynucleotidyl transferase-mediated deoxyuridine (TdT) triphosphate (dUTP) nick end labeling (TUNEL) assay and sperm chromatin dispersion (SCD) test. However, studying on sperm DNA might be very complex because the sperm DNA differs from the somatic cell DNA with its unique structure. Also, the sperm DNA undergoes many changes during spermatogenesis and it is condensed by being packaged tightly with different types and numbers of protamines in different species. Despite all these difficulties, these methods provide important information about the reasons and consequences of DNA damages in sperm and the effects of these damages on reproduction.

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“…In this respect, the assessment of sperm DNA fragmentation (SDF) as a potential predictor of fertility is gaining increased acceptance; [5][6][7] in fact, we would suggest that this might particularly be the case for predictions of pregnancy based on intracytoplasmic sperm injection (ICSI), as this procedure requires no sperm motility and a single 'selected' spermatozoon, so that the effect of other sperm population parameters on reproductive outcome are largely negated or reduced. Under these conditions, it is possible that sperm DNA fragmentation is likely to be a better predictor of male factor infertility, especially that associated with compromised syngamy and early embryonic loss, [8][9][10][11] although in other cases, this relationship is still not clear.…”

Section: Introductionmentioning

confidence: 99%

Can DNA fragmentation of neat or swim-up spermatozoa be used to predict pregnancy following ICSI of fertile oocyte donors?

Gosálvez

Caballero²,

López‐Fernández³

et al. 2013

Asian J Androl

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This study compared the potential of assessing sperm DNA fragmentation (SDF) from neat semen and the subsequent swim-up (SU) procedure to predict pregnancy when conducting ICSI of fertile donor oocytes. Infertile females (n581) were transferred embryos resulting from intracytoplasmic sperm injection (ICSI) of their partner's spermatozoa and proven donor oocytes. This model normalized the impact of female factor in putative sperm DNA repair. Semen was blindly assessed for SDF using Halosperm immediately following ejaculation (NS) and after swim-up at the time of ICSI fertilisation. There was a decrease in SDF values of the ejaculated semen sample following the swim-up protocol (P50.000). Interestingly, pregnancy could be equally predicted from SDF values derived from either neat or swim-up semen samples. Receiver operator curves and the derived Youden's indices determined SDF cutoff values for NS and SU of 24.8% and 17.5%, respectively. Prediction of pregnancy from NS SDF had a sensitivity of 75% and a specificity of 69%, whereas for SU SDF was 78% and 73%, respectively. While increased levels of SDF negatively impact reproductive outcome, we have shown that a reduction in SDF following sperm selection using ICSI with proven donor oocytes is not mandatory for achieving pregnancy. This suggests that a certain level of DNA damage that is not detectable using current technologies could be impacting on the relative success of assisted reproductive technology (ART) procedures. Consequently, we propose a modification of the so called 'iceberg model' as a possible rationale for understanding the role of SDF in reproductive outcome.

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Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications

Cited by 19 publications

References 114 publications

Incipient post‐zygotic barrier in a model system of ecological speciation with gene flow

Incipient post‐zygotic barrier in a model system of ecological speciation with gene flow

Sperm DNA and detection of DNA fragmentations in sperm

Can DNA fragmentation of neat or swim-up spermatozoa be used to predict pregnancy following ICSI of fertile oocyte donors?

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