Sperm chromatin assessment

Agarwal, Ashok; Tsarev, Igor; Ērenpreiss, Juris; Sharma, Rakesh

doi:10.3109/9781841849713.006

Cited by 18 publications

(18 citation statements)

References 223 publications

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“…Heads of the spermatozoa with high chromatin DNA integrity are stained blue and damaged DNA are stained purple. It is a rapid and simple assay [29] , [30] .…”

Section: Different Techniques Of Sdf Assays For Measuring Dna Damagementioning

confidence: 99%

“…Further, it is washed in Tris–borate-ethylenediamine tetra-acetic acid (EDTA) buffer for 2 min and rehydrated in ascending grades of ethanol (70%, 90% and 100%). Finally the spermatozoa are stained with nuclear stain DAPI (4′,6-diamidino-2-phenylindole) and observed under a fluorescence microscope [30] , [42] , [44] .…”

Section: Different Techniques Of Sdf Assays For Measuring Dna Damagementioning

confidence: 99%

“…After the completion of electrophoresis the slides are stained with SYBR Green I to visualise fragmented DNA under a fluorescence microscope. The results are analysed based on the tail length either manually or using specialised commercially available software [30] .…”

Section: Different Techniques Of Sdf Assays For Measuring Dna Damagementioning

confidence: 99%

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A systematic review on sperm DNA fragmentation in male factor infertility: Laboratory assessment

Selvam

Agarwal

2018

Arab Journal of Urology

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ObjectiveTo review sperm DNA fragmentation (SDF) testing as an important sperm function test in addition to conventional semen analysis. High SDF is negatively associated with semen quality, the fertilisation process, embryo quality, and pregnancy outcome. Over recent decades, different SDF assays have been developed and reviewed extensively to assess their applicability and accuracy as advanced sperm function tests. Amongst them, the standardisation of the terminal deoxynucleotidyl transferased UTP nick-end labelling (TUNEL) assay with a bench top flow cytometer in clinical practice deserves special mention with a threshold value of 16.8% to differentiate infertile men with DNA damage from fertile men.Materials and methodsA systematic literature search was performed through the PubMed, Medline, and ScienceDirect databases using the keywords ‘sperm DNA fragmentation’ and ‘laboratory assessment’. Non-English articles were excluded and studies related to humans were only included.ResultsOf the 618 identified, 87 studies (original research and reviews) and in addition eight book chapters meeting the selection criteria were included in this review. In all, 366 articles were rejected in the preliminary screening and a further 165 articles related to non-human subjects were excluded.ConclusionThere are pros and cons to all the available SDF assays. TUNEL is a reliable technique with greater accuracy and as an additional diagnostic test in Andrology laboratories along with basic semen analysis can predict fertility outcome, and thus direct the choice of an assisted reproductive technology procedure for infertile couples. Also, the TUNEL assay can be used as a prognostic test and results are beneficial in deciding personalised treatment for infertile men.

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“…Heads of the spermatozoa with high chromatin DNA integrity are stained blue and damaged DNA are stained purple. It is a rapid and simple assay [29] , [30] .…”

Section: Different Techniques Of Sdf Assays For Measuring Dna Damagementioning

confidence: 99%

Section: Different Techniques Of Sdf Assays For Measuring Dna Damagementioning

confidence: 99%

See 1 more Smart Citation

A systematic review on sperm DNA fragmentation in male factor infertility: Laboratory assessment

Selvam

Agarwal

2018

Arab Journal of Urology

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“…It may result in suboptimal embryogenesis and/or increased risk of mutations to the offspring (21) The exact mechanism by which DNA damage arises in human spermatozoa is not clearly understood and three mechanisms have been proposed: defective sperm chromatin packaging, apoptosis, and oxidative stress (22).…”

Section: Mortalities and Morbiditiesmentioning

confidence: 99%

The Impact of Bacterial Infections on Human Spermatozoa

Zeyad¹,

Amor²,

Hammadeh³

2017

International Journal of Women’s Health and Reproduction Scienc

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“…It is essential to determine the level of DNA fragmentation of freeze-dried sperm before use. Sperm chromatin assessment is an independent measure of sperm quality that provides better diagnostic and prognostic capabilities for potential fertility than standard sperm parameters [15]. Janny and Menezo reported that development to the blastocyst stage is linked to sperm quality [16] and it has been reported that increased levels of DNA fragmentation significantly decrease embryonic development to the blastocyst stage in mice [17].…”

Section: Establishing a Methods For Evaluating Dna Fragmentation Of Frmentioning

confidence: 99%

A Study on Freeze-Drying as a Method of Preserving Mouse Sperm

Kawase¹,

Suzuki

2011

Journal of Reproduction and Development

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Abstract. This review describes the study of freeze-dried mouse sperm for practical application in preserving and transporting genetic resources. Freeze-dried sperm can be used to preserve and transport genetic resources; however, there still remain many areas which need to be studied. In particular, it is essential to assure long-term preservation over several decades or centuries. Recently, the theory of accelerated degradation kinetics to freeze-dried mouse sperm has been applied, and found that long-term preservation by conventional methods requires temperatures lower than -80 C. When the relationship between the pressure at primary drying and the preservation potential of freezedried mouse sperm was examined, a pressure of 0.37 mbar at primary drying significantly improved the developmental rate to the blastocyst stage. In addition, it has been shown that freeze-dried sperm stored at -80 C with and without transportation can retain their ability to generate viable offspring after storage for up to 2 years. Sperm chromatin structure assay (SCSA) was applied to mouse sperm freeze-dried under several conditions and compared the results with the embryonic developmental rates of freeze-dried sperm after intracytoplasmic sperm injection (ICSI) and with comet assay results. Furthermore, SCSA might be useful for estimation of developmental potential of fertilized eggs derived from ICSI using freeze-dried sperm in mice.

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Sperm chromatin assessment

Cited by 18 publications

References 223 publications

A systematic review on sperm DNA fragmentation in male factor infertility: Laboratory assessment

A systematic review on sperm DNA fragmentation in male factor infertility: Laboratory assessment

The Impact of Bacterial Infections on Human Spermatozoa

A Study on Freeze-Drying as a Method of Preserving Mouse Sperm

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