Sperm activation and effects of cryopreservation on motility, ultrastructure and DNA integrity in Grey mullet Mugil cephalus

Balamurugan, R.; Prapaporn, Whaiprib; Munuswamy, Natesan

doi:10.1016/j.aqrep.2019.100204

Cited by 15 publications

(23 citation statements)

References 56 publications

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“…tail, which showed similarity with the cryopreserved sperm of gray mullet (Balamurugan et al, 2019). In the present study, comet assay results (Figure 3) the DNA integrity of gray mullet, rainbow trout and Atlantic croaker's sperm (Balamurugan et al, 2019).…”

Section: F I G U R Esupporting

confidence: 78%

“…This technique was performed via a CometAssay ® reagent kit (Trevigen Inc.), following the protocol described by Kim et al (2016). tail, which showed similarity with the cryopreserved sperm of gray mullet (Balamurugan et al, 2019). In the present study, comet assay results (Figure 3) the DNA integrity of gray mullet, rainbow trout and Atlantic croaker's sperm (Balamurugan et al, 2019).…”

Section: F I G U R Ementioning

confidence: 55%

“…Mitochondrial function and cell viability can act as indicators of the quality of cryopreserved sperm, which may degrade during freezing and thawing (Kang et al, 2004). DNA integrity acts as a quality indicator of cryopreservation succession (Balamurugan, Prapaporn, & Munuswamy, 2019), and it can assess by measuring the % DNA in the comet tail.…”

Section: F I G U R Ementioning

confidence: 99%

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Effects of 3 years of cryopreservation on sperm quality of seven‐band grouper, Epinephelus septemfasciatus

2020

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Section: F I G U R Esupporting

confidence: 78%

Section: F I G U R Ementioning

confidence: 55%

Section: F I G U R Ementioning

confidence: 99%

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Effects of 3 years of cryopreservation on sperm quality of seven‐band grouper, Epinephelus septemfasciatus

2020

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“…Sperm DNA integrity acts as an indicator of cryopreservation succession. It can be assessed with comet assay (Balamurugan et al, 2019). DNA fragmentation is responsible for reduced fertilization capacity and abnormal embryonic development (Gwo et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

et al. 2021

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Pacific abalone, Haliotis discus hannai, is a high commercial seafood in South-East Asia. The aim of the present study was to determine effects of cryopreservation on gene expression and post thaw sperm quality of Pacific abalone. Two ions, Na+ (459.1 ± 3.1 mM) and Cl– (515.9 ± 1.1 mM), were predominant in the seminal plasma (pH: 6.8 ± 0.1; osmolarity: 1,126 ± 3 mOsmL–1). Cryopreservation reduced mRNA expression levels of protein kinase A (PKA-C) and heat shock proteins (HSP70 and HSP90) genes in sperm. Fluorescent technique was used to compare morphological defects, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and DNA integrity of sperm cryopreserved with five different cryopreservation solutions (8% Me2SO, 8% EG, 6% PG, 2% GLY, and 2% MeOH). Droplet in tail and coiled tail defects was not observed for sperm cryopreserved with 8% Me2SO or 2% GLY. Sperm cryopreserved with 8% Me2SO showed improved DNA integrity and lower cryodamage than sperm cryopreserved with other cryoprotectants. Sperm to egg ratio of 10,000:1 was found to be the most suitable ratio for in vitro fertilization among different ratios tested. The fertilization rate of sperm cryopreserved with 8% Me2SO was not significantly (p > 0.05) different from that of sperm cryopreserved with 2% GLY. DNA fragmentation showed strongly negative relationships with sperm quality parameters. Sperm cryopreserved with 8% Me2SO showed higher post thaw quality and mRNA expression of sperm motility associated gene than those cryopreserved with other cryoprotectants. The present research suggests to use 8% Me2SO for cryopreservation of Pacific abalone sperm as well as for hatchery production.

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“…Sperm DNA integrity acts as one of the indicators of fertility potential. It can be assessed by comet assay [17], sperm chromatin structure assay (SCSA) [18], sperm chromatin dispersion (SCD) test [19], and TUNEL assay (terminal deoxynucleotidyl transferase-mediated dUDP nick end labelling) [20]. DNA fragmentation is known to reduce the fertilization capacity of sperm.…”

Section: Introductionmentioning

confidence: 99%

Effects of Antifreeze Protein III on Sperm Cryopreservation of Pacific Abalone, Haliotis discus hannai

Hossen

Sharker

Cho

et al. 2021

IJMS

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Pacific abalone (Haliotis discus hannai) is a highly commercial seafood in Southeast Asia. The aim of the present study was to improve the sperm cryopreservation technique for this valuable species using an antifreeze protein III (AFPIII). Post-thaw sperm quality parameters including motility, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), DNA integrity, fertility, hatchability, and mRNA abundance level of heat shock protein 90 (HSP90) were determined to ensure improvement of the cryopreservation technique. Post-thaw motility of sperm cryopreserved with AFPIII at 10 µg/mL combined with 8% dimethyl sulfoxide (DMSO) (61.3 ± 2.7%), 8% ethylene glycol (EG) (54.3 ± 3.3%), 6% propylene glycol (PG) (36.6 ± 2.6%), or 2% glycerol (GLY) (51.7 ± 3.0%) was significantly improved than that of sperm cryopreserved without AFPIII. Post-thaw motility of sperm cryopreserved with 2% MeOH and 1 µg/mL of AFPIII was also improved than that of sperm cryopreserved without AFPIII. A combination of 10 µg/mL AFPIII with 8% DMSO resulted in the highest post-thaw motility, showing AI of 60.1 ± 3.9%, PMI of 67.2 ± 4.0%, and MMP of 59.1 ± 4.3%. DNA integrity of sperm cryopreserved using 10 µg/mL AFPIII combined with 8% DMSO was not significantly (p > 0.05) different from that of fresh sperm. Cryopreservation using a combination of AFPIII with 8% DMSO improved fertilization and hatching rates of sperm compared to that of cryopreservation without supplementation of 10 µg/mL AFPIII. Sperm cryopreserved using AFPIII showed higher mRNA abundance levels of HSP90 than those cryopreserved without AFPIII. Results of the present study suggest that 10 µg/mL AFPIII combined with 8% DMSO can be used for large scale cryopreservation of Pacific abalone sperm and for hatchery production.

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Sperm activation and effects of cryopreservation on motility, ultrastructure and DNA integrity in Grey mullet Mugil cephalus

Cited by 15 publications

References 56 publications

Effects of 3 years of cryopreservation on sperm quality of seven‐band grouper, Epinephelus septemfasciatus

Effects of 3 years of cryopreservation on sperm quality of seven‐band grouper, Epinephelus septemfasciatus

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

Effects of Antifreeze Protein III on Sperm Cryopreservation of Pacific Abalone, Haliotis discus hannai

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