Effects of 3 years of cryopreservation on sperm quality of seven‐band grouper,
            <i>Epinephelus septemfasciatus</i>

Kim, Soo Cheol; Hossen, Shaharior; Kho, Kang Hee

doi:10.1111/are.14615

Cited by 8 publications

(13 citation statements)

References 12 publications

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“…Abnormalities including total structure damage, abnormal body shape, coiled tail, and droplet in tail were only observed for cryopreserved sperm. Morphological abnormalities were previously reported from cryopreserved sperm of zebrafish and seven-band grouper (Costa et al, 2020;Kim et al, 2020b). Cryo-injury of post thaw sperm can occur due to several causative factors such as cold shock, ice crystallization, CPA toxicity, pH fluctuation, and osmotic effect (Chao and Liao, 2001;Chatiza et al, 2011;FIGURE 9 | Fertilization and hatching rates of cryopreserved sperm of Pacific abalone (n = 3).…”

Section: Discussionmentioning

confidence: 91%

“…Fluorescent technique was applied to evaluate morphological characteristics (AI, PMI, and MMP) of fresh and cryopreserved sperm according to the method described by Kim et al (2020b). Sperm structure was visualized under a fluorescent microscope (100×).…”

Section: Morphological Evaluation Of Spermmentioning

confidence: 99%

“…Sperm DNA integrity was assessed using Comet assay R kit by slightly modifying the protocol described by Kim et al (2020b). Briefly, sperm sample was suspended with pre-chilled PBS (1X) at a concentration of 1 × 10 5 cells/mL.…”

Section: Sperm Dna Integrity Testmentioning

confidence: 99%

“…Sperm motility is essential to transport male DNAs to eggs for both external and internal fertilization (Sukhan et al, 2020). Recently, fluorescent technique has been applied to detect post thaw sperm quality including acrosome integrity (AI), plasma membrane integrity (PMI), and mitochondrial membrane potential (MMP) (Pereira et al, 2010;Liu et al, 2014;Kim et al, 2020b). Cryopreservation decreases the metabolic activity and post thaw sperm quality by altering mitochondrial membrane and structure (Madeddu et al, 2010;Figueroa et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

et al. 2021

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Pacific abalone, Haliotis discus hannai, is a high commercial seafood in South-East Asia. The aim of the present study was to determine effects of cryopreservation on gene expression and post thaw sperm quality of Pacific abalone. Two ions, Na+ (459.1 ± 3.1 mM) and Cl– (515.9 ± 1.1 mM), were predominant in the seminal plasma (pH: 6.8 ± 0.1; osmolarity: 1,126 ± 3 mOsmL–1). Cryopreservation reduced mRNA expression levels of protein kinase A (PKA-C) and heat shock proteins (HSP70 and HSP90) genes in sperm. Fluorescent technique was used to compare morphological defects, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and DNA integrity of sperm cryopreserved with five different cryopreservation solutions (8% Me2SO, 8% EG, 6% PG, 2% GLY, and 2% MeOH). Droplet in tail and coiled tail defects was not observed for sperm cryopreserved with 8% Me2SO or 2% GLY. Sperm cryopreserved with 8% Me2SO showed improved DNA integrity and lower cryodamage than sperm cryopreserved with other cryoprotectants. Sperm to egg ratio of 10,000:1 was found to be the most suitable ratio for in vitro fertilization among different ratios tested. The fertilization rate of sperm cryopreserved with 8% Me2SO was not significantly (p > 0.05) different from that of sperm cryopreserved with 2% GLY. DNA fragmentation showed strongly negative relationships with sperm quality parameters. Sperm cryopreserved with 8% Me2SO showed higher post thaw quality and mRNA expression of sperm motility associated gene than those cryopreserved with other cryoprotectants. The present research suggests to use 8% Me2SO for cryopreservation of Pacific abalone sperm as well as for hatchery production.

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Section: Discussionmentioning

confidence: 91%

Section: Morphological Evaluation Of Spermmentioning

confidence: 99%

Section: Sperm Dna Integrity Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

et al. 2021

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“…4.10. Comet Assay to Detect Sperm DNA Integrity of Fresh and Cryopreserved Sperm Comet assay was performed according to the method described by Kim et al [56]. A Comet Assay ® kit was used to assess DNA integrity of fresh and cryopreserved sperm.…”

Section: Fluorescent Technique To Assess Pmi Ai and Mmp Of Cryopreserved Spermmentioning

confidence: 99%

Effects of Antifreeze Protein III on Sperm Cryopreservation of Pacific Abalone, Haliotis discus hannai

Hossen

Sharker

Cho

et al. 2021

IJMS

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Pacific abalone (Haliotis discus hannai) is a highly commercial seafood in Southeast Asia. The aim of the present study was to improve the sperm cryopreservation technique for this valuable species using an antifreeze protein III (AFPIII). Post-thaw sperm quality parameters including motility, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), DNA integrity, fertility, hatchability, and mRNA abundance level of heat shock protein 90 (HSP90) were determined to ensure improvement of the cryopreservation technique. Post-thaw motility of sperm cryopreserved with AFPIII at 10 µg/mL combined with 8% dimethyl sulfoxide (DMSO) (61.3 ± 2.7%), 8% ethylene glycol (EG) (54.3 ± 3.3%), 6% propylene glycol (PG) (36.6 ± 2.6%), or 2% glycerol (GLY) (51.7 ± 3.0%) was significantly improved than that of sperm cryopreserved without AFPIII. Post-thaw motility of sperm cryopreserved with 2% MeOH and 1 µg/mL of AFPIII was also improved than that of sperm cryopreserved without AFPIII. A combination of 10 µg/mL AFPIII with 8% DMSO resulted in the highest post-thaw motility, showing AI of 60.1 ± 3.9%, PMI of 67.2 ± 4.0%, and MMP of 59.1 ± 4.3%. DNA integrity of sperm cryopreserved using 10 µg/mL AFPIII combined with 8% DMSO was not significantly (p > 0.05) different from that of fresh sperm. Cryopreservation using a combination of AFPIII with 8% DMSO improved fertilization and hatching rates of sperm compared to that of cryopreservation without supplementation of 10 µg/mL AFPIII. Sperm cryopreserved using AFPIII showed higher mRNA abundance levels of HSP90 than those cryopreserved without AFPIII. Results of the present study suggest that 10 µg/mL AFPIII combined with 8% DMSO can be used for large scale cryopreservation of Pacific abalone sperm and for hatchery production.

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Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus)

Chen

et al. 2023

Aquaculture Reports

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Effects of 3 years of cryopreservation on sperm quality of seven‐band grouper, Epinephelus septemfasciatus

Cited by 8 publications

References 12 publications

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

Effects of Antifreeze Protein III on Sperm Cryopreservation of Pacific Abalone, Haliotis discus hannai

Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus)

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