Spent growth medium of Pantoea agglomerans primes wheat suspension cells for augmented accumulation of hydrogen peroxide and enhanced peroxidase activity upon elicitation

Ortmann, Imke; Moerschbacher, Bruno M.

doi:10.1007/s00425-006-0271-7

Cited by 38 publications

(32 citation statements)

References 47 publications

(40 reference statements)

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“…Ortmann et al . also reported the priming activity of EPS from Pantoea agglomerans on the ROS generation and peroxidase activity in several plant cells using similar model system [25], [26].…”

Section: Discussionmentioning

confidence: 85%

Positive Crosstalk of MAMP Signaling Pathways in Rice Cells

et al. 2012

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Plants have evolved efficient defense mechanisms known as priming and synergy, both of which can mobilize defense responses more extensively against successive pathogen invasion or simultaneous stimulation by different signal molecules. However, the mechanisms underlying these phenomena were largely unknown. In the present study, we used cultured rice cells and combination of purified MAMP molecules as a model system to study the mechanisms of these phenomena. We found that the pretreatment of rice cells with a low concentration of bacterial lipopolysaccharide (LPS) apparently primed the defense responses induced by successive N-acetylchitooctaose (GN8) treatment. On the other hand, simultaneous treatment with GN8 and LPS also resulted in the similar enhancement of defense responses observed for the LPS-induced priming, indicating that the synergistic effects of these MAMPs are basically responsible for such enhancement of defense responses, though the effect could be interpreted as “priming” under some experimental conditions. These results also indicate that such a positive crosstalk of signaling cascade downstream of MAMP receptors seems to occur very rapidly, probably at early step(s) of signaling pathway. Comprehensive analysis of phytohormones revealed a specific enhancement of the synthesis of jasmonic acid (JA), both in the LPS pretreatment and also simultaneous treatment, indicating a role of JA in the enhancement of downstream responses.

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Section: Discussionmentioning

confidence: 85%

Positive Crosstalk of MAMP Signaling Pathways in Rice Cells

et al. 2012

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“…It is reported that Pantoea sp. can effectively mobilize cellular to defense responses upon pathogen attack, such as wheat rust (Ortmann and Moerschbacher, 2006). There are other reports that the Pantoea sp.…”

Section: Dgge and Diversity Analysismentioning

confidence: 99%

Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere

Bai

Jin

et al. 2010

Journal of Environmental Sciences

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Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the γ-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application.

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“…However, when highly purified and homogeneous elicitors are used, the intensity of the oxidative burst induced can reach in some cases only 10% or less of that induced by crude elicitor preparations, suggesting the existence of additional signal perception mechanisms in the plant cell involved in the full induction of that response. [38][39][40] In our experiments, chitosan polymers with DA 69% and chitosan oligomers with DA 78% elicited the highest generation of H 2 O 2 . To our knowledge, such a thorough comparison between chitosan polymers and oligomers of different DA has not been previously reported for the induction of an oxidative burst in suspension cultured cells, nor for any other of the many biological activities of chitosan.…”

Section: Discussionmentioning

confidence: 99%

Partially Acetylated Chitosan Oligo- and Polymers Induce an Oxidative Burst in Suspension Cultured Cells of the Gymnosperm Araucaria angustifolia

et al. 2008

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Suspension-cultured cells were used to analyze the activation of defense responses in the conifer A. angustifolia , using as an elicitor purified chitosan polymers of different degrees of acetylation (DA 1-69%), chitin oligomers of different degrees of polymerization (DP 3-6), and chitosan oligomer of different DA (0-91%). Suspension cultured cells elicited with chitosan polymers reacted with a rapid and transient generation of H2O2, with chitosans of high DA (60 and 69%) being the most active ones. Chitosan oligomers of high DA (78 and 91%) induced substantial levels of H2O2, but fully acetylated chitin oligomers did not. When cultivated for 24-72 h in the presence of 1-10 microg mL(-1) chitosan (DA 69%), cell cultures did not show alterations in the levels of enzymes related to defense responses, suggesting that, in A. angustifolia , the induction of an oxidative burst is not directly coupled to the induction of other defense reactions.

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Spent growth medium of Pantoea agglomerans primes wheat suspension cells for augmented accumulation of hydrogen peroxide and enhanced peroxidase activity upon elicitation

Cited by 38 publications

References 47 publications

Positive Crosstalk of MAMP Signaling Pathways in Rice Cells

Positive Crosstalk of MAMP Signaling Pathways in Rice Cells

Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere

Partially Acetylated Chitosan Oligo- and Polymers Induce an Oxidative Burst in Suspension Cultured Cells of the Gymnosperm Araucaria angustifolia

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