Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere

Gu, Li; Bai, Zhihui; Jin, Bo; Hu, Qing; Wang, Huili; Zhuang, Guoqiang; Zhang, Hongxun

doi:10.1016/s1001-0742(09)60084-x

Cited by 44 publications

(46 citation statements)

References 34 publications

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“…Chemical treatments used in the grapevine protection program affected the microbial populations on and inside plant leaves during the growing season, and modified the balance between pathogenic and beneficial microorganisms (63). Likewise, the fungicides enostroburin and metalaxyl caused changes in the bacterial communities of the wheat and pepper phyllosphere (9,11), as well as the insecticides cypermethrin, abamectin, and imidacloprid in the communities of the pepper, cucumber, and broccoli phyllosphere (11,12). However, the impacts of chemical and biological treatments on the phyllosphere microbiota are dependent on the dosage and frequency of applications and the mechanisms of action of the products tested, as well as the types of indigenous microorganisms and the weather conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Many active molecules have a wide spectrum of activity and might affect nontarget microorganisms within the ecosystem, including those on the plant (12). The impact of chemical treatments has been evaluated on the microbial structure of soil (13,14) and aquatic ecosystems (15), while only a few studies have addressed the impact of pesticides on nontarget microorganisms of the phyllosphere (9,11,12). The use of biological control agents offers a promising alternative or supplement to chemical fungicides for the control of crop diseases.…”

mentioning

confidence: 99%

“…Therefore, the composition of phyllosphere communities could be affected by environmental factors, such as UV radiation, air pollution, and nitrogen fertilization, as well as by biotic factors, such as plant species and invading microorganisms (6)(7)(8). Moreover, the phyllosphere is an open system and microbes can invade plant leaves by migration from the atmosphere, soil, other plants, insects, and animals (9).…”

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confidence: 99%

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Resilience of the Natural Phyllosphere Microbiota of the Grapevine to Chemical and Biological Pesticides

Perazzolli

Antonielli

Storari

et al. 2014

Appl Environ Microbiol

145

122

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The phyllosphere is colonized by complex microbial communities, which are adapted to the harsh habitat. Although the role and ecology of nonpathogenic microorganisms of the phyllosphere are only partially understood, leaf microbiota could have a beneficial role in plant growth and health. Pesticides and biocontrol agents are frequently applied to grapevines, but the impact on nontarget microorganisms of the phyllosphere has been marginally considered. In this study, we investigated the effect of a chemical fungicide (penconazole) and a biological control agent (Lysobacter capsici AZ78) on the leaf microbiota of the grapevine at three locations. Amplicons of the 16S rRNA gene and of the internal transcribed spacer were sequenced for bacterial and fungal identification, respectively. Pyrosequencing analysis revealed that the richness and diversity of bacterial and fungal populations were only minimally affected by the chemical and biological treatments tested, and they mainly differed according to grapevine locations. Indigenous microbial communities of the phyllosphere are adapted to environmental and biotic factors in the areas where the grapevines are grown, and they are resilient to the treatments tested. The biocontrol properties of phyllosphere communities against downy mildew differed among grapevine locations and were not affected by treatments, suggesting that biocontrol communities could be improved with agronomic practices to enrich beneficial populations in vineyards. Plants support a complex micro-ecosystem, and they host distinct bacterial communities on and inside various plant organs (1). The aerial part of plants (phyllosphere) is normally colonized by a variety of bacteria, filamentous fungi and yeasts (2). Microbial phyllosphere communities are complex and composed by many uncultured microorganisms (2, 3), which are adapted to the harsh environmental conditions (4, 5). In particular, microbial epiphytes of the phyllosphere are exposed to the atmosphere and must deal with direct UV radiation, wide fluctuations in temperature, low water availability, and limited access to nutrients (2,6). Therefore, the composition of phyllosphere communities could be affected by environmental factors, such as UV radiation, air pollution, and nitrogen fertilization, as well as by biotic factors, such as plant species and invading microorganisms (6-8). Moreover, the phyllosphere is an open system and microbes can invade plant leaves by migration from the atmosphere, soil, other plants, insects, and animals (9).The phyllosphere has been less intensively studied than the rhizosphere and has received considerable attention in recent years (1). The interest in phyllosphere microbiology was initially driven by investigations into plant pathogens, but most phyllosphere-colonizing microorganisms live as commensals and/or mutualistic symbionts on their host plants (2,4,6). Phyllosphere communities are involved in functional processes as large in scale as the carbon cycle, nitrogen fixation, and degradation of organic pollu...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Resilience of the Natural Phyllosphere Microbiota of the Grapevine to Chemical and Biological Pesticides

Perazzolli

Antonielli

Storari

et al. 2014

Appl Environ Microbiol

145

122

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“…Firstly, by removing fungal-bacterial antagonism, fungicides also free an advantageous niche for bacteria [4,5]. Secondly, the antibacterial activity is demonstrated predominantly by metal-based pesticides, the usage of which in the contemporary rural economy significantly decreased as they were substituted by triazoles, benzimidazoles and thiocarbamates [1,2,29].…”

Section: Fungicidesmentioning

confidence: 99%

“…But insecticides, fungicides, herbicides use in agriculture against target objects do not provide the removal of bacteria from ecosystem. Therefore, pesticides violate the ecological balance between bacterial and fungal microbiota, particularly that do not exhibit antibacterial action [4,5]. As result is growing problem of bacterial diseases.…”

Section: Introductionmentioning

confidence: 99%

Specifics of pesticides effects on the phytopathogenic bacteria

Patyka

Buletsa

Pasichnyk

et al. 2016

Ecological Chemistry and Engineering S

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Abstract:The data concerning the effects of pesticides of different nature on the phytopathogenic bacteria was examined and summarized. Without extensive research on the mechanisms of interaction between pathogenic bacteria and pesticides in the literature review a similar message about microorganisms of soil and phyllosphere are included. The bacteria can be suppressed permanently by pesticides with a mechanism of action that universally affects biological processes in living systems. Long-term storage, ease of use and fast visible effect are the advantages of synthetic pesticides remedies. But chemical pollution, shifts in the balance of ecosystems, unpredictable effects of chemical pesticides on non-target objects are the drawbacks. Stimulation of resistance response in plants is unifying factor for all types of biopesticides. This is realized through localization of the pathogen during infection, blocking its further penetration, distribution and reproduction. The results of the study of effects of plant protection products on the phytopathogenic bacteria of main crops are described. Among all tested pesticides, thiocarbamate fungicides demonstrated significant inhibitory action on phytopathogens, but their effect may be neutralized by other constituents of multicomponent preparations. Triazole fungicides affect the causative agents of bacterioses of crops at a dose of more than 1% of the active substance in the nutrient medium. Insecticides and herbicides have little or no effect on phytopathogenic bacteria; however they can enhance morphological dissociations of some Pseudomonas strains, thereby increasing their ability to survive. The disadvantage of many biopesticides against phytopathogenic microorganisms is the difference between their efficacy in vitro and in vivo that is why the desired result is not achieved in field condition. In addition, biological pesticides often lose their activity causing the problem of constant search for new active antagonists. The fact that the sensitivity of phytopathogenic bacteria to pesticides is strain-dependent should be considered in practice, particularly, assessment of the antibacterial action of various preparations should not be limited to a single bacterial strain.

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