Speleothem and biofilm formation in a granite/dolerite cave, Northern Sweden

Sallstedt, Therese; Ivarsson, Magnus; Lundberg, Johannes; Sjöberg, Rabbé; Romaní, Juan Ramón Vidal

doi:10.5038/1827-806x.43.3.7

Cited by 19 publications

(21 citation statements)

References 26 publications

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Section: Introductionmentioning

confidence: 99%

“…The process of speleothem formation in this cave is of particular interest because the main speleothem forming mineral is calcite, which is not reported as a major speleothem forming mineral in granite caves [ 5 ]. A previous study combining Environmental Scanning Electron Microscope (ESEM) and fluorescent microscopy on this cave has shown an abundance of fungi and Actinobacteria that together play important roles in the speleothem life cycle through a constructive-destructive interplay [ 5 ]. Actinobacteria are commonly identified in cave biofilms [ 11 ], speleothems, and cave soils from various locations, and can induce mineral precipitation [ 12 , 13 ], while fungi are mostly destructive agents [ 14 ] due to chemical etching and physical breakdown of the mineral substrate.…”

Section: Introductionmentioning

confidence: 99%

“…It has been suggested that the speleothem microbiome from Tjuv-Ante’s cave consists of heterotrophs probably living of organic matter transported from the surface by percolating fluids [ 5 ]. However, it is not known if there are any chemoautotrophs in the dolerite that can act as a carbon source for the heterotrophic communities on the cave walls.…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic Analysis from the Interior of a Speleothem in Tjuv-Ante's Cave, Northern Sweden

et al. 2016

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Speleothems are secondary mineral deposits normally formed by water supersaturated with calcium carbonate percolating into underground caves, and are often associated with low-nutrient and mostly non-phototrophic conditions. Tjuv-Ante’s cave is a shallow-depth cave formed by the action of waves, with granite and dolerite as major components, and opal-A and calcite as part of the speleothems, making it a rare kind of cave. We generated two DNA shotgun sequencing metagenomic datasets from the interior of a speleothem from Tjuv-Ante’s cave representing areas of old and relatively recent speleothem formation. We used these datasets to perform i) an evaluation of the use of these speleothems as past biodiversity archives, ii) functional and taxonomic profiling of the speleothem’s different formation periods, and iii) taxonomic comparison of the metagenomic results to previous microscopic analyses from a nearby speleothem of the same cave. Our analyses confirm the abundance of Actinobacteria and fungi as previously reported by microscopic analyses on this cave, however we also discovered a larger biodiversity. Interestingly, we identified photosynthetic genes, as well as genes related to iron and sulphur metabolism, suggesting the presence of chemoautotrophs. Furthermore, we identified taxa and functions related to biomineralization. However, we could not confidently establish the use of this type of speleothems as biological paleoarchives due to the potential leaching from the outside of the cave and the DNA damage that we propose has been caused by the fungal chemical etching.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metagenomic Analysis from the Interior of a Speleothem in Tjuv-Ante's Cave, Northern Sweden

et al. 2016

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“…In addition, it seems that the conditions of their origin and development was created as a result of their combination and cementing, including a potential microbiological influence (Borsato et al, 2000;Moral et al, 2012;Sallstedt et al, 2014). XRD analysis confirmed these as 100% calcite (Leél-Őssy et al, 2011;Bieniok et al, 2011).…”

Section: Mineralogical Depositsmentioning

confidence: 99%

Complex speleogenetic processes and mineral deposition in the Caucasus region of Georgia

Asanidze¹,

Chikhradze²,

Lezhava³

et al. 2017

JEB

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Aim: Methodology:Results: Interpretation:The present study aimed to characterize the speleogenesis, associated karst morphological processes and rare mineral deposition in Muradi Cave, which is located in the Caucasus region of Georgia. In this research, it was important to make basic survey and mapping of the Muradi Cave. Also, one of the goals was to collect information from a speleological point of view in order to understand the speleogenetic processes.Multiple methods were utilized to investigate Muradi Cave, including both physical and geochemical analyses, to characterize the cave's origin, morphology and mineralogical deposits. The morphological parameters and tectonic directions of Muradi Cave were identified by compass-clinometer and laser distance meter, from which we compiled the schematic plan and 3D model of the cave. Air temperature was measured in two different places within the cave using an Onset HOBO Pro V2 Data Logger. Speleothem mineralogy was analyzed using XRD.In the present study, recently investigated Muradi Cave in the Racha limestone massif, which contains different speleological, mineralogical and hydrological features providing insight to its formation and possible influences on cave development in the region. The uniqueness of Muradi Cave is given by the fact that it contains almost all types and subtypes of speleothems and sediments recorded nowadays in the caves of Caucasus. The mineral aggregates found in Muradi Cave are rare for the caves of the Caucasus. By taking into consideration, the geological-geomorphological peculiarities and the results of the archeological materials of the region, it became possible the identification of the age of the oldest deposits.Collectively, this investigation of an investigated area in Georgia, as well as the discovery of unique formations, provide new insight on the development of large limestone massifs in the region and has implications for deep cave development and the understanding of the region's speleological and geomorphological evolution.

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“…Известно, что полимерные вещества стимулируют образование биопленок и способствуют активному функционированию микробного сообщества [41]. Во многих случаях микроорганизмы и их внеклеточные полимерные вещества действуют как эффективные центры формирования новых структур, которые могут привести к пассивной инкрустации био пленок и повлиять на структуру натечных образований [46]. оптической плотности, был установлен в присутствии ДЭ и не зависел от температуры.…”

Section: физиологические особенности развития мк «лунное молоко»unclassified

Микроорганизмы И Образование Спелеотемы «лунное Молоко» В Карстовых Пещерах

Кондратьева¹,

Полевская²

2017

Biosfera

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Геологи и спелеологи исследуют карстовые пещеры, рассматривая их внутренние пейзажи в макромасштабе. Для оценки динамики биогеохимических процессов, происходящих в пещерах в контактной зоне вода-порода в микромасштабе, проведено экспериментальное исследование трансформации карбонатов кальция при участии микробных комплексов, входящих в состав натечного образования (спелеотемы) «лунное молоко» из карстовой пещеры Снежная (Абхазия). Интенсивную адгезию микроорганизмов за счет продуцирования полимерного матрикса и образование биопленок на поверхности стекла наблюдали на 30-е сутки в присутствии дрожжевого экстракта при 23 °С независимо от концентрации кальцита. Наиболее активная агрегация кристаллов карбоната кальция и их растворение происходили под влиянием слизистых полимеров. Полное растворение 1 г/л кристаллов кальцита бактериальным консорциумом «лунное молоко» наблюдали при 4 °С. С помощью сканирующей электронной микроскопии были показаны разные этапы формирования биопленок и характер растворения кальцита. Дополнительное внесение примесей органических веществ стимулировало растворение кристаллов. Рассмотрены предпосылки для возможной кальцификации порового пространства карстовых пещер под влиянием экологических факторов.

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Speleothem and biofilm formation in a granite/dolerite cave, Northern Sweden

Cited by 19 publications

References 26 publications

Metagenomic Analysis from the Interior of a Speleothem in Tjuv-Ante's Cave, Northern Sweden

Metagenomic Analysis from the Interior of a Speleothem in Tjuv-Ante's Cave, Northern Sweden

Complex speleogenetic processes and mineral deposition in the Caucasus region of Georgia

Микроорганизмы И Образование Спелеотемы «лунное Молоко» В Карстовых Пещерах

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