Spectrum of Virus Inhibition by Consensus Interferon YM643

Yasuda, Shuhei; Huffman, John H.; Smee, Donald F.; Sidwell, Robert W.; Miyata, Keiji

doi:10.1177/095632020001100504

Cited by 10 publications

(3 citation statements)

References 18 publications

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“…It has been synthetized by Dr Gunter Bartels ASM Germany, on 08/09/2006 and is a derivative of the compounds previously synthetized and found active on Rhinovirus HRV14 and HRV39. The positive control is the Pirodavir (Janssen Pharmaceutics) [8][9][10][11][12].…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

Anti-Rhinovirus Activity of Ethyl 4-(3-(2-(3-Methylisoxazol-5-Yl) Ethoxy) Propoxy) Benzoate (EMEB)

Tarro¹

2015

Pharm Anal Acta

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The compuond EMEB has got a definite anti-Rhinovirus activity on both HRV14 (group A) and HRV39 (group B). The specific activity is lower than that found for Pirodavir used as a positive control, but, since the cytotoxic activity of EMEB on human HeLa cells is more favourable than that of Pirodavir (50 µg/ml against 3 µg/ml), the final Protection Index is higher for EMEB (> 700) as compared to Pirodavir (250). EMEB seems to be stable in aqueous solutions, since its activity after 10 days was unchanged. When EMEB is challanged with Rhinovirus infected HeLa cells during the whole reproduction cicle, its antiviral activity remains evident and strong even after 18 hours from infection. This fact is important because it means that the compound keeps functioning even when the viral infection is already in progress; this finding makes us to hypothesize that the compound EMEB could act not only as a prophylactic agent against the common cold, but also as a therapeutic drug in patients who already show the disease symptoms (at least within the first 24 hours from the start of symptoms). These last statements must be confirmed with assays on the mechanism of action of the compound, by analysing its adhesion to the cell virus internalization into the cells, the viral uncoating, transcription and translation, and finally on viral morphogenesis.

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Section: Chemicals and Reagentsmentioning

confidence: 99%

Anti-Rhinovirus Activity of Ethyl 4-(3-(2-(3-Methylisoxazol-5-Yl) Ethoxy) Propoxy) Benzoate (EMEB)

Tarro¹

2015

Pharm Anal Acta

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“…Interferon alfacon-1 is a consensus-type interferon that has been efficacious in the clinical treatment of hepatitis C virus (HCV) infections in human patients (Melian and Plosker, 2001;Sjogren et al, 2005;Yasuda and Miyata, 2002), as well as in treatment of various viral diseases and cancer in experimental animal models or cell culture models (Hisaka et al, 2004;Morrey et al, 2004a;Takemoto et al, 2004;Yasuda et al, 2000). This cytokine works well in humans as well as in hamsters against different viral diseases (Balan et al, 2006;Gowen et al, 2005;Morrey et al, , 2004a).…”

Section: Introductionmentioning

confidence: 99%

Effect of exogenous interferon and an interferon inducer on western equine encephalitis virus disease in a hamster model

Julander

Siddharthan

Blatt³

et al. 2007

Virology

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Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infection. Hamsters were highly sensitive to intraperitoneal (i.p.) infection with WEEV. Disease progression was rapid, and virus titers in serum, brain, liver, and kidney of infected hamsters peaked between 2 and 4 days post-virus inoculation (dpi). Foci of virus infection were detected in neurons of the cerebral cortex and midbrain. Pre-treatment i.p. with either interferon alfacon-1 (5 microg/kg/day) or with Ampligen (3.2 mg/kg/day) resulted in complete survival, reduced brain titers, and improved weight gain. This model of WEEV infection in hamsters appears to serve as a suitable model for the evaluation of potential therapeutic agents for the treatment of WEE disease.

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“…3 Enteroviruses of the Picornavirus family are small, nonenveloped, positive-sensed, single-stranded RNA viruses, which are classically among the most sensitive to type I IFN, with encephalomyocarditis virus (EMCV) being one of the most IFN sensitive of the enteroviruses. 4 …”

mentioning

confidence: 99%