Spectrum of Microbial Isolates from Wound Infections in Patients admitted in a Tertiary Care Hospital, Kolkata

Abstract

Increasing emergence of drug-resistant bacteria is becoming a major problem globally. A retrospective study of 790 culture and sensitivity reports on microbial isolates, from infected wounds was carried out in KPC Medical College and Hospital, Jadavpur, Kolkata, India. 504 patients were males and 286 females. Gram negative organisms were isolated in 561 and gram positive in 229. Among the gram negative organisms, Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli were the most common, whereas Staph aureus was the only gram positive organism isolated. Antibiotic sensitivity tests revealed most gram negative organisms susceptible to carbapenems, polymyxin and colistin. Staphylococci showed sensitivity to tigecycline, clindamycin, vancomycin, teicoplanin and linezolid. Interesting differences in the type of organisms isolated in male and female patients were noted. For example Enterococcus faecalis was found only in female patients. Increasing resistance of microorganisms cultured from infected wounds to first line and even second line antibiotics is a matter of great concern and can be attributed to indiscriminate and irrational use of broad spectrum antibiotics. This has to stop forthwith if we want to prevent resistance to antibiotics; otherwise morbidity, mortality and health care costs in treating patients with wound infections are going to increase exponentially

Curcumin assisted green synthesis of silver and zinc oxide nanostructures and their antibacterial activity against some clinical pathogenic multi-drug resistant bacteria

“…86 Antimicrobial resistance in E. coli has increased over the world. E. coli were resistant to tetracycline, cefotaxime, ciprooxacin, levooxacin and ceazidime about 100%, 100%, 86.7%, 86.7%, 73.3% respectively, however showed sensitivity to imipenem (93.3%), meropenem (80.0%), tigecycline (66.7%), cefoxitin (66.7%) and amikacin (60.0%) this results similar to previous studies reported by Saha et al, 87 and Yakha et al 88 Such studies stated that imipenem and amikacin are very effective drugs against E. coli isolates. In our study, the multidrug resistance ability within all identied bacteria was noticed.…”

Antimicrobial Activity of Typha angustata Bory and Chaub Inflorescence Against Wound Associated Bacteria

“…Escherichia coli isolates sensitivity against levofloxacin was found to be (92.3%) as shown in. Similar observations have been reported byYakha et al(2015);Gomatheswari andJeyamurugan (2017), andSaha et al(2017) that imipenem, amikacin, and gentamycin were very effective drugs against E. coli isolates but presented resistance against cefuroxime, ciprofloxacin, ofloxacin, cefotaxime, and ampicillin.…”

“…Hooper and Jacoby (2015) reported that resistance is due to changes in DNA gyrase enzyme and/or the topoisomerase enzymes or by the defective function of porine channels. Saha et al (2017) and Bhalchandra et al (2018) reported that P. aeruginosa strains were sensitive to levofloxacin, polymyxinb and ciprofloxacin and showed high resistance against ceftazidme and aztreonam. The diameters of the inhibition zones were interpreted according to CLSI ( 2016), The isolates were reported as R-resistant, I-intermediate and S-Sensitive.…”

Evaluation of antimicrobial activity of some plant extracts against antibiotic susceptible and resistant bacterial strains causing wound infection

“…This resistance is due to β-lactamase production (extended-spectrum beta lactamases) that hydrolyze these drugs (Palzkill, 2018). Earlier studies by Saha et al (2017) and Bhalchandra et al(2018) reported that P. aeruginosa strains were sensitive to polymyxinb, levofloxacin, and ciprofloxacin and showed high resistance against Aztreonam and ceftazidime. Yakha et al (2015) and Perimet al (2015) described high resistance against ceftazidime and polymyxin B.…”