Background: Due to rapid development of microbial resistance against chemotherapeutic agents (mostly antibiotics), it has become essential currently to screen effective, safe, cheap, and available therapeutics from various medicinal plants-like herbs-for their potential antimicrobial effect. Aim: To estimate the antibacterial activity of aqueous, ethanol, and methanol extracts of each of Moringa oleifera L. leaves and Matricaria recutita L. flowers against antibiotic-resistant and sensitive bacterial strains isolated from patients having wound infections. Results: In the present study, a total of one hundred clinical samples were obtained from different cases of infected wounds. Forty isolates (40%) of pure bacterial cultures were detected. Pseudomonas aeruginosa was found to be the predominant agent isolated from the wound infections (32.5%) followed by Staphylococcus spp. (25%), E. coli (20%), Klebsiella spp. (20%), and Proteus mirabilis (2.5%). Sensitivity of the bacterial isolates was tested against antibiotic discs: piperacillin, , and clindamycin. Out of the 40 bacterial strains studied, 20 isolates were multidrug-resistant (MDR), 7 extensively drug-resistant (XDR) and 3 were pan drug-resistant (PDR). The in vitro susceptibility test showed that the water, ethanol (95%), and methanol (80%) extracts of Moringa oleifera L.(leaves) and Matricaria recutita L.(flowers) produced an inhibitory effect against 12 resistant MDR, XDR, and PDR test isolates, with minimum inhibitory concentration (MIC) ranging from 7.8-62.5 mg/ml. Water and methanol extracts of both plants represented good activity against most of the sensitive and resistant isolates whereas ethanol extract of both plants showed a lesser activity against nearly all of the isolates Conclusion: This study had the potential value to develop antibacterial agents against resistant (MDR, XDR, and PDR) and susceptible bacteria supporting the significant use of plant extracts in treating wound infections related to bacteria and these active extracts will provide useful information for discovering new compounds with better activity and more effective against resistant (MDR, XDR, and PDR) and susceptible bacteria responsible for wound infections than currently available antibiotic agents.

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