Spectroscopy in the analysis of bacterial and eukaryotic cell footprints on implant surfaces

Kaivosoja, Emilia; Virtanen, Sannakaisa; Rautemaa, Riina; Lappalainen, Reijo; Yt, Konttinen

doi:10.22203/ecm.v024a05

Cited by 7 publications

(5 citation statements)

References 60 publications

(43 reference statements)

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“…For example, the zeta-potential for laser-ablated DLC was higher (-54.5±0.6 mV) 13 than for DLC film fabricated using filtered pulsed arc discharge (-70.2±1.1 mV, both measured in 1mM KCl, pH 7) 15 . This will most likely be reflected also as differences in the surface chemistries between the two samples, which could then translate into different electrochemical behavior during cyclic 12 voltammetry.…”

Section: Dopaminequinone Can Undergo a Chemical Reaction By Intramolementioning

confidence: 91%

Carbon thin films as electrode material in neural sensing

Kaivosoja

Sainio

Lyytinen

et al. 2014

Surface and Coatings Technology

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For therapeutic purposes, an accurate measurement of dopamine levels in situ would be highly desirable. A novel strategy for the selective determination of dopamine concentrations based on carbon thin film electrodes is presented in this paper. Traditionally, in order to make diamond films conductive, they are doped with different concentration of boron atoms. Here, carbon thin films with varying conductivities were achieved by unbalanced magnetron sputtering. The benefit of the method is that it can be performed at room temperature consequently broadening the selection of suitable substrates. The carbon thin films had a wide potential window, which showed strong dependence on conductivity. The potential window was largest (4.6 V) with the 2 most resistive carbon thin film. On the other hand, the sensitivity of the electrode towards dopamine was not significantly affected by the conductivity. In addition, relatively similar behavior with respect to the dopamine oxidation was observed between various surfaces. The slight differences observed in the electrochemical behavior among the thin films are most likely caused by 1) different conductivities and/or 2) different surface charges and subsequent differences in the chemical properties of the surfaces. In conclusion, it can be stated that a-C thin film is a very potential neural sensing material.

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Section: Dopaminequinone Can Undergo a Chemical Reaction By Intramolementioning

confidence: 91%

Carbon thin films as electrode material in neural sensing

Kaivosoja

Sainio

Lyytinen

et al. 2014

Surface and Coatings Technology

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“…Identifying mono-, di-, oligo-, and polysaccharides spectroscopically is often important for understanding the properties of formulated products . X-ray core level spectroscopies are structurally incisive methods that are increasingly used for the analysis of interfacial species in complex products, devices, and biomaterials. − The most commonly used variant, X-ray photoelectron spectroscopy (XPS), provides information on the chemical state of atoms through sensitivity to the local electron density variations caused by changes in chemical bonding, which lead to chemical shifts of core level binding energies, and interpretation can be complemented or enhanced with the results of density functional theory (DFT) calculations . Careful C 1s core level binding energy measurements by XPS of fructose, xylose, glucose, galactose, maltose, α-lactose, β-lactose, and cellulose have previously revealed that XPS is sensitive enough to distinguish mono-, di-, and polysaccharides…”

Section: Introductionmentioning

confidence: 99%

NEXAFS Sensitivity to Bond Lengths in Complex Molecular Materials: A Study of Crystalline Saccharides

et al. 2015

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Detailed analysis of the C K near-edge X-ray absorption fine structure (NEXAFS) spectra of a series of saccharides (fructose, xylose, glucose, galactose, maltose monohydrate, α-lactose monohydrate, anhydrous β-lactose, cellulose) indicates that the precise determination of IPs and σ* shape resonance energies is sensitive enough to distinguish different crystalline saccharides through the variations in their average C-OH bond lengths. Experimental data as well as FEFF8 calculations confirm that bond length variations in the organic solid state of 10(-2) Å can be experimentally detected, opening up the possibility to use NEXAFS for obtaining incisive structural information for molecular materials, including noncrystalline systems without long-range order such as dissolved species in solutions, colloids, melts, and similar amorphous phases. The observed bond length sensitivity is as good as that originally reported for gas-phase and adsorbed molecular species. NEXAFS-derived molecular structure data for the condensed phase may therefore be used to guide molecular modeling as well as to validate computationally derived structure models for such systems. Some results indicate further analytical value in that the σ* shape resonance analysis may distinguish hemiketals from hemiacetals (i.e., derived from ketoses and aldoses) as well as α from β forms of otherwise identical saccharides.

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“…For example, diamond-like carbon resisted microbial attachment in static conditions compared to titanium and tantalum, − and ta-C resisted protein attachment compared to platinum . The sensitivity of ta-C is usually not high enough for biological applications, but functionalization of the ta-C surface with different carbon allotropes, such as carbon nanotubes and nanofibers or nanodiamonds (ND), has shown great potential for the electrochemical detection of different biomolecules, such as DA, glutamate, and ascorbic acid .…”

mentioning

confidence: 99%

Electrochemical Fouling of Dopamine and Recovery of Carbon Electrodes

et al. 2017

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A significant problem with implantable sensors is electrode fouling, which has been proposed as the main reason for biosensor failures in vivo. Electrochemical fouling is typical for dopamine (DA) as its oxidation products are very reactive and the resulting polydopamine has a robust adhesion capability to virtually all types of surfaces. The degree of DA fouling of different carbon electrodes with different terminations was determined using cyclic voltammetry (CV) and scanning electrochemical microscopy (SECM) approach curves and imaging. The rate of electron transfer kinetics at the fouled electrode surface was determined from SECM approach curves, allowing a comparison of insulating film thickness for the different terminations. SECM imaging allowed the determination of different morphologies, such as continuous layers or islands, of insulating material. We show that heterogeneous modification of carbon electrodes with carboxyl-amine functionalities offers protection against formation of an insulating polydopamine layer, while retaining the ability to detect DA. The benefits of the heterogeneous termination are proposed to be due to the electrostatic repulsion between amino-functionalities and DA. Furthermore, we show that the conductivity of the surfaces as well as the response toward DA was recovered close to the original performance level after cleaning the surfaces for 10-20 cycles in HSO on all materials but pyrolytic carbon (PyC). The recovery capacity of the PyC electrode was lower, possibly due to stronger adsorption of DA on the surface.

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Spectroscopy in the analysis of bacterial and eukaryotic cell footprints on implant surfaces

Cited by 7 publications

References 60 publications

Carbon thin films as electrode material in neural sensing

Carbon thin films as electrode material in neural sensing

NEXAFS Sensitivity to Bond Lengths in Complex Molecular Materials: A Study of Crystalline Saccharides

Electrochemical Fouling of Dopamine and Recovery of Carbon Electrodes

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