Spectroscopic techniques as a diagnostic tool for early detection of osteoporosis

Abstract

Osteoporosis (OP) a kind of bone disease, is very serious in particular for old persons, and may lead them to immobility and death. Early detection of the diseases is the first consideration for the patients to have more options to live a healthy life. The biomarkers or bonemarkers provide a promising challenge in clinical proteomics for early disease detection. In this paper, optical techniques such as Fourier Transform Infrared Spectroscopy (FTIR) and UV/Visible spectroscopy are employed to find the bone markers and emphasis has been given on noninvasive modalities for early detection of osteoporosis. Blood plasma samples procured from two groups, patients and healthy persons were tested. Both of the optical techniques revealed obvious differences in the spectra; between two groups, for example, increase in intensity for OP persons. New peaks were found at 1646, 1540, 1456 and 1077 cm-1 in FTIR spectra. Except 1588 cm-1, we showed decrease in spectral intensity of OP persons. In UV/Visible spectroscopy results, new peaks appeared in the OP patients spectra at the wavelength of 279 nm and 414 nm. These differences in the spectra of the two types samples, allow rapid and cost-effective discrimination of the potential patients with the optical techniques which were verified by the bone densitometer in the hospitals. The new and novel technique is quick, reliable and effective.

Investigating optimum sample preparation for infrared spectroscopic serum diagnostics

“…9 However, the most popular biouids analysed by vibrational spectroscopy are blood-derived products such as plasma [10][11][12][13][14] and serum. [15][16][17][18] Proof-of-principle studies have shown the ability of FTIR spectroscopy to identify different types of cancer from serum samples.…”

Study of bioactive glass ceramic for use as bone biomaterial in vivo: Investigation by nuclear magnetic resonance and histology

“…generates a smaller formation of the silica rich layer [7]. Four months later, we saw the disappearance of the peaks of Na [18][19]. After four and nine months, the crystallization and the changing of 46S6-10Zn is seemed by the appearance of all peaks characterizing biological apatite.…”

Fabrication method, structure, mechanical, and biological properties of decellularized extracellular matrix for replacement of wide bone tissue defects

“…In both FTIR spectra, the peak at 1655-1675 cm À 1 is identified as being associated with the C¼O carbonyl functional group of proteins in the type 1 of amide compound. After decellularization, the absorption band characteristic of the C¼O bond from lipids shifted from 1715 to 1730 cm À 1 to the region of 1720-1740 cm À 1 (Singh et al, 2010). The N-H and C-N groups from amide 2 were observed to have characteristic peaks at 1566 and 1598 cm À 1 for the intact and decellularized bone, respectively.…”