Fabrication method, structure, mechanical, and biological properties of decellularized extracellular matrix for replacement of wide bone tissue defects

Anisimova, Natalia; Kiselevsky, M. V.; Сухорукова, И. В.; Shvindina, N.V.; Shtansky, Dmitry V.

doi:10.1016/j.jmbbm.2015.05.009

Cited by 18 publications

(12 citation statements)

References 56 publications

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“…Many studies have focused on the decellularization of natural tissues and organs, including the blood vessel [2], skin [3], small intestinal submucosa [4], urinary bladder [5], adipose tissue [6], spleen [7], and lung [8]. Their shortcomings include the limited amount of autogenous tissue derived from the patient, increased operation time, postoperative recovery time, and surgical complication such as risk of blood loss, wound infection, pain, shock, and functional damage in the donor part of the body [9]. Furthermore, current decellularization techniques are not able to remove the cellular components completely.…”

Section: Introductionmentioning

confidence: 99%

The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

Zhou

Wang

Zhang

et al. 2020

Stem Cells International

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To date, the decellularized scaffold has been widely explored as a source of biological scaffolds for regenerative medicine. However, the acellular matrix derived from natural tissues and organs has a lot of defects, including the limited amount of autogenous tissue and surgical complication such as risk of blood loss, wound infection, pain, shock, and functional damage in the donor part of the body. In this study, we prepared acellular matrix using adipose-derived stem cell (ADSC) sheets and evaluate the cellular compatibility and immunoreactivity. The ADSC sheets were fabricated and subsequently decellularized using repeated freeze-thaw, Triton X-100 and SDS decellularization. Oral mucosal epithelial cells were seeded onto the decellularized ADSC sheets to evaluate the cell replantation ability, and silk fibroin was used as the control. Then, acellular matrix was transplanted onto subcutaneous tissue for 1 week or 3 weeks; H&E staining and immunohistochemical analysis of CD68 expression and quantitative real-time PCR (qPCR) were performed to evaluate the immunogenicity and biocompatibility. The ADSC sheet-derived ECM scaffolds preserved the three-dimensional architecture of ECM and retained the cytokines by Triton X-100 decellularization protocols. Compared with silk fibroin in vitro, the oral mucosal epithelial cells survived better on the decellularized ADSC sheets with an intact and consecutive epidermal cellular layer. Compared with porcine small intestinal submucosa (SIS) in vivo, the homogeneous decellularized ADSC sheets had less monocyte-macrophage infiltrating in vivo implantation. During 3 weeks after transplantation, the mRNA expression of cytokines, such as IL-4/IL-10, was obviously higher in decellularized ADSC sheets than that of porcine SIS. A Triton X-100 method can achieve effective cell removal, retain major ECM components, and preserve the ultrastructure of ADSC sheets. The decellularized ADSC sheets possess good recellularization capacity and excellent biocompatibility. This study demonstrated the potential suitability of utilizing acellular matrix from ADSC sheets for soft tissue regeneration and repair.

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Section: Introductionmentioning

confidence: 99%

The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

Zhou

Wang

Zhang

et al. 2020

Stem Cells International

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“…Decellularized Bone and Matrix. Decellularized bone modeling involves decellularizing native bone tissue via chemical, enzymatic, physical or combination treatments 9,10 and has recently been explored for developing in vitro cancer-and-bone models. 11 These models circumvent immunogenicity caused by xenografts, mimic bone structure and microarchitecture, and offer cells a tissue-appropriate template.…”

Section: D Models Using Naturally Derived Materialsmentioning

confidence: 99%

Tissue-engineered 3D cancer-in-bone modeling: silk and PUR protocols

et al. 2016

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Cancers that metastasize or grow in the bone marrow are typically considered incurable and cause extensive damage to the bone and bone marrow. The bone is a complex, dynamic, three-dimensional (3D) environment composed of a plethora of cells that may contribute to, or constrain, the growth of tumor cells and development of bone disease. The development of safe and effective drugs is currently hampered by pre-clinical two-dimensional (2D) models whose poor predictive power does not accurately predict the success or failure of therapeutics. These inadequate models often result in drugs proceeding through extensive pre-clinical studies only to fail clinically. Consistently, 3D co-culture systems prove superior to 2D mono-cultures in modeling cell phenotypes, disease progression and response to therapeutics. As a complex, multicellular, multidimensional bone microenvironment, 3D models allow for more accurate predictions of tumor growth, cell-cell and cell-matrix interactions, and resulting therapeutic responses. In this review we will discuss various 3D models available and describe step-by-step protocols for two of the most well-established 3D culture models for studying tumor-induced bone disease.

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“…Porous scaffold has been used in tissue engineering to provide an appropriate environment and architecture for the tissue regeneration and mechanical properties control [ 1 , 2 , 3 ]. Compared with conventional manufacturing techniques, additive manufacturing (AM) techniques exhibited attractive attention for the abilities of constructing complex geometries [ 4 ], such as topology optimization structure and bionic bone trabeculae structure [ 5 ]. AM technologies mainly included stereolithography (STL) of a photosensitive resin, fused filament fabrication (FFF) of polymer and selective laser sintering (SLS) of metal powder [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Raster Angle and Material Components on Mechanical Properties of Polyether-Ether-Ketone/Calcium Silicate Scaffolds

et al. 2021

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Polyetheretherketone (PEEK) was widely used in the fabrication of bone substitutes for its excellent chemical resistance, thermal stability and mechanical properties that were similar to those of natural bone tissue. However, the biological inertness restricted the osseointegration with surrounding bone tissue. In this study, calcium silicate (CS) was introduced to improve the bioactivity of PEEK. The PEEK/CS composites scaffolds with CS contents in gradient were fabricated with different raster angles via fused filament fabrication (FFF). With the CS content ranging from 0 to 40% wt, the crystallinity degree (from 16% to 30%) and surface roughness (from 0.13 ± 0.04 to 0.48 ± 0.062 μm) of PEEK/CS scaffolds was enhanced. Mechanical testing showed that the compressive modulus of the PEEK/CS scaffolds could be tuned in the range of 23.3–541.5 MPa. Under the same printing raster angle, the compressive strength reached the maximum with CS content of 20% wt. The deformation process and failure modes could be adjusted by changing the raster angle. Furthermore, the mapping relationships among the modulus, strength, raster angle and CS content were derived, providing guidance for the selection of printing parameters and the control of mechanical properties.

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Fabrication method, structure, mechanical, and biological properties of decellularized extracellular matrix for replacement of wide bone tissue defects

Cited by 18 publications

References 56 publications

The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine

Tissue-engineered 3D cancer-in-bone modeling: silk and PUR protocols

Effects of Raster Angle and Material Components on Mechanical Properties of Polyether-Ether-Ketone/Calcium Silicate Scaffolds

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