Spectroscopic study on the interaction between mononaphthalimide spermidine (MINS) and bovine serum albumin (BSA)

Tian, Zhiyong; Zang, Fenglei; Luo, Wen; Zhao, Zhonghua; Wang, Yueqiao; Xu, Xuejun; Wang, Chaojie

doi:10.1016/j.jphotobiol.2014.10.013

Cited by 103 publications

(42 citation statements)

References 49 publications

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“…2B) showed a good linearity and the K SV values decreased with increasing temperature ( Table 1), suggesting that the fluorescence quenching mechanism may be static quenching. Moreover, K q values (1.31 Â 10 12 , 0.73 Â 10 12 , 0.65 Â 10 12 and 0.62 Â 10 12 L mol À1 s À1 at 292, 298, 304 and 310 K, respectively) were higher than the maximum scatter collision-quenching constant for various quenchers with biopolymers, 2.0 Â 10 10 L mol À1 s À1 , indicating that static quenching was the probable quenching mechanism of PSO-trypsin interaction [31,32].…”

Section: Fluorescence Spectra Studiesmentioning

confidence: 89%

Binding characteristics of psoralen with trypsin: Insights from spectroscopic and molecular modeling studies

Liu

Zhang

Liao

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Section: Fluorescence Spectra Studiesmentioning

confidence: 89%

Binding characteristics of psoralen with trypsin: Insights from spectroscopic and molecular modeling studies

Liu

Zhang

Liao

et al. 2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…The MRE value calculated these Equation .

MRE = \frac{Observed CD (mdeg)}{C p \times h \times l \times 10}

\propto - helix (%) = \frac{- MR {normalE}_{210} - 4000}{33000 - 4000} \times 10

where θ obs is the observed ellipticity in millidegrees, c is the concentration of BSA, l is the path length, and n is the number of amino acid residues …”

Section: Methodsmentioning

confidence: 99%

In Vitro Study of Ethyl‐4‐(3,4.5‐trimethoxyphenyl)‐2,7,7‐trimethyl‐5‐oxo1,4,5,6,7,8‐hexahydroquinoline‐3‐carboxylate and Bovine Serum Albumin Using Multi‐Spectroscopic Techniques and Molecular Docking

Kumbhar¹,

Gore

Choudhari

et al. 2019

Macromolecular Symposia

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The binding of quinolone derivative ethyl‐4‐(3,4.5‐trimethoxyphenyl)‐2,7,7‐trimethyl‐5‐oxo1,4,5,6,7,8‐hexahydroquinoline‐3‐carboxylate (ETMTMHQC) to bovine serum albumin (BSA) is investigated by various spectroscopic methods and molecular docking analysis. The fluorescence quenching spectroscopic results show that ETMTMHQC bind to the protein BSA. The binding constant value is found to be 5.2 × 10−6 K (mol dm3). The thermodynamic parameter of the system shows increase in temperature with gradual decrease in Stern–Volmer quenching constant thereby indicating static quenching mode. Negative entropy and positive enthalpy indicate the hydrogen bonding interaction. The (r) distance between BSA and ETMTMHQC obtained from fluorescence resonance energy transfer is found to be 7.0 nm. The UV–visible spectra reveal the increase in absorbance on formation of BSA–ETMTMHQC complex. The CD spectral study indicates reduction of α‐helical structure in BSA and small changes in the tertiary structure of the protein. ETMTMHQC interacts strongly with BSA, and small changes in protein morphology are advised by molecular docking results. Moreover, docking results show that ETMTMHQC binds to BSA at ASN390 residue.

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“…34 The inset in Figure 2 gives K SV values from which k q can be calculated. In some cases, the fluorophore, with the same quencher, can be quenched by a combination of static and dynamic quenching mechanism (combined quenching process).…”

Section: Fluorescence Quenching Mechanismmentioning

confidence: 99%

A Study of the Interaction Betweentrans-Dehydrocrotonin, a Bioactive Natural 19-nor-Clerodane, and Serum Albumin

Chaves¹,

Soares²,

Maciel³

et al. 2016

Journal of the Brazilian Chemical Society

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Spectroscopic study on the interaction between mononaphthalimide spermidine (MINS) and bovine serum albumin (BSA)

Cited by 103 publications

References 49 publications

Binding characteristics of psoralen with trypsin: Insights from spectroscopic and molecular modeling studies

Binding characteristics of psoralen with trypsin: Insights from spectroscopic and molecular modeling studies

In Vitro Study of Ethyl‐4‐(3,4.5‐trimethoxyphenyl)‐2,7,7‐trimethyl‐5‐oxo1,4,5,6,7,8‐hexahydroquinoline‐3‐carboxylate and Bovine Serum Albumin Using Multi‐Spectroscopic Techniques and Molecular Docking

A Study of the Interaction Betweentrans-Dehydrocrotonin, a Bioactive Natural 19-nor-Clerodane, and Serum Albumin

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