Spectroscopic Studies of Cholesterol: Fourier Transform Infra-Red and Vibrational Frequency Analysis

Gupta, Ujval; Singh, V. B.; Kumar, Vinay; Khajuria, Yugal

doi:10.1166/mat.2014.1161

Cited by 76 publications

(40 citation statements)

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“…8a. The absorptions peaks reveal the presence of cholesterol molecule present in the gallstone sample and are in good agreement with the experimental data reported by Gupta et al and co-workers for cholesterol molecule [19]. The broad absorption peak at 204 nm is due the -C=C-band present in the cholesterol molecule.…”

Section: Uv-vis Spectroscopy Of Cholesterol Gallstonesupporting

confidence: 90%

Multi-spectroscopic analysis of cholesterol gallstone using TOF-SIMS, FTIR and UV–Vis spectroscopy

et al. 2015

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Section: Uv-vis Spectroscopy Of Cholesterol Gallstonesupporting

confidence: 90%

Multi-spectroscopic analysis of cholesterol gallstone using TOF-SIMS, FTIR and UV–Vis spectroscopy

et al. 2015

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“…Cholesterol spectrum shows an absorption band at 3419 cm −1 due to O-H stretching. The bands in the region of 2800-3000 cm −1 are attributed to asymmetric and symmetric stretching vibrations of CH 2 and CH 3 groups [59]. The spectrum of the liposome physical mixture shows characteristic bands at 2926, 2854, 1740, 1635, 1616 and 1599 cm −1 due to the various absorption bands of its components.…”

Section: Particle Size and Polydispersity Index Of Liposome And Ethosmentioning

confidence: 96%

Liposomal and Ethosomal Gels for the Topical Delivery of Anthralin: Preparation, Comparative Evaluation and Clinical Assessment in Psoriatic Patients

2020

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To enhance anthralin efficacy against psoriasis and reduce its notorious side effects, it was loaded into various liposomal and ethosomal preparations. The nanocarriers were characterized for drug encapsulation efficiency, size, morphology and compatibility between various components. Optimum formulations were dispersed in various gel bases and drug release kinetics were studied. Clinical efficacy and safety of liposomal and ethosomal Pluronic®F-127 gels were evaluated in patients having psoriasis (clinicaltrials.gov identifier is NCT03348462). Safety was assessed by recording various adverse events. Drug encapsulation efficiency ≥97.2% and ≥77% were obtained for liposomes and ethosomes, respectively. Particle sizes of 116 to 199 nm and 146 to 381 nm were observed for liposomes and ethosomes, respectively. Fourier-Transform infrared (FT-IR) spectroscopy and differential scanning calorimetry (DSC) studies confirmed the absence of interaction between anthralin and various nanocarrier components. Tested gel bases showed excellent ability to sustain drug release. At baseline, the patients had a median Psoriasis Area and Severity Index (PASI) of 3.4 for liposomes and 3.6 for ethosomes without significant difference. After treatment, mean PASI change was −68.66% and −81.84% for liposomes and ethosomes, respectively with a significant difference in favor of ethosomes. No adverse effects were detected in both groups. Anthralin ethosomes could be considered as a potential treatment of psoriasis.

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“…The FT-IR spectra of quercetin exhibited its distinguishing characteristic peaks at 3289.58 cm −1 (-OH stretching), 1672.73 cm −1 (C=O stretching), 1521.9 cm −1 (aromatic C=C stretching) and 1212 cm −1 (aromatic C-O stretching) [45,46]. Cholesterol showed its characteristic peaks at 3405.24 cm −1 (-OH stretching), 2866.69 cm −1 (CH 2 symmetric stretching vibration), 1466.54 cm −1 (asymmetric stretching vibration of CH 2 and CH 3 ), 1376.82 cm −1 (CH 2 and CH 3 bending vibration) and 1056.58 cm −1 (C-O bending vibration) [47,48].…”

Section: Ft-ir Studiesmentioning

confidence: 99%

Quercetin Loaded Monolaurate Sugar Esters-Based Niosomes: Sustained Release and Mutual Antioxidant—Hepatoprotective Interplay

et al. 2020

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Flavonoids possess different interesting biological properties, including antibacterial, antiviral, anti-inflammatory and antioxidant activities. However, unfortunately, these molecules present different bottlenecks, such as low aqueous solubility, photo and oxidative degradability, high first-pass effect, poor intestinal absorption and, hence, low systemic bioavailability. A variety of delivery systems have been developed to circumvent these drawbacks, and among them, in this work niosomes have been selected to encapsulate the hepatoprotective natural flavonoid quercetin. The aim of this study was to prepare nanosized quercetin-loaded niosomes, formulated with different monolaurate sugar esters (i.e., sorbitan C12; glucose C12; trehalose C12; sucrose C12) that act as non-ionic surfactants and with cholesterol as stabilizer (1:1 and 2:1 ratio). Niosomes were characterized under the physicochemical, thermal and morphological points of view. Moreover, after the analyses of the in vitro biocompatibility and the drug-release profile, the hepatoprotective activity of the selected niosomes was evaluated in vivo, using the carbon tetrachloride (CCl 4 )-induced hepatotoxicity in rats. Furthermore, the levels of glutathione and glutathione peroxidase (GSH and GPX) were measured. Based on results, the best formulation selected was glucose laurate/cholesterol at molar ratio of 1:1, presenting spherical shape and a particle size (PS) of 161 ± 4.6 nm, with a drug encapsulation efficiency (EE%) as high as 83.6 ± 3.7% and sustained quercetin release. These niosomes showed higher hepatoprotective effect compared to free quercetin in vivo, measuring serum biomarker enzymes (i.e., alanine and aspartate transaminases (ALT and AST)) and serum biochemical parameters (i.e., alkaline phosphatase (ALP) and total proteins), while following the histopathological investigation. This study confirms the ability of quercetin loaded niosomes to reverse CCl 4 intoxication and to carry out an antioxidant effect.Pharmaceutics 2020, 12, 143 2 of 18 and anticancer activities [1][2][3]. These intrinsic properties, together with their biocompatibility and remarkable antioxidant activity, have attracted researchers' attention to explore potential therapeutic applications [4,5].Quercetin (3, 30, 4, 5, 7-pentahydroxyflavone) is probably the most studied bioflavonoid, belonging to the family of flavonols; its therapeutic applications include hepatoprotection [6], prevention of neural cell apoptosis [7] and cancer chemo-prevention and treatment [8].Quercetin acts as strong antioxidant by scavenging free radicals and transition metal ions, thus decreasing the process of lipid peroxidation, which is responsible for the development of many diseases, e.g., cardiovascular and neurodegenerative diseases, as well as liver damage [9][10][11].However, quercetin therapeutic applications present challenges due to (i) low aqueous solubility, (ii) photo and oxidative degradability, (iii) high first-pass effect, (iv) poor intestinal absorption and, hence, low systemic...

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Spectroscopic Studies of Cholesterol: Fourier Transform Infra-Red and Vibrational Frequency Analysis

Cited by 76 publications

References 1 publication

Multi-spectroscopic analysis of cholesterol gallstone using TOF-SIMS, FTIR and UV–Vis spectroscopy

Multi-spectroscopic analysis of cholesterol gallstone using TOF-SIMS, FTIR and UV–Vis spectroscopy

Liposomal and Ethosomal Gels for the Topical Delivery of Anthralin: Preparation, Comparative Evaluation and Clinical Assessment in Psoriatic Patients

Quercetin Loaded Monolaurate Sugar Esters-Based Niosomes: Sustained Release and Mutual Antioxidant—Hepatoprotective Interplay

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