Spectroscopic Estimation of Tenofovir Alafenamide, an antiretroviral drug

Kumar, P V Nagendra; Jacob, Jane T; Ajina, Khadjeeth; Jazeela,; Fathima,

doi:10.5958/0974-360x.2016.00101.3

Cited by 6 publications

(2 citation statements)

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“…Tenofovir alafenamide is related to a HIV drugs groups which called nucleoside reverse transcriptase inhibitors (NRTIs) which acts by blocking reverse transcriptase, to prevent HIV from multiplying 1 . The literature revealed that various predetermination methods of tenofovir alafenamide either alone or with other drugs by spectroscopic methods [2][3][4][5] , HPLC [6][7][8][9][10][11][12][13][14][15] , LC 16 , LC-MS/ MS 17,18 . However, a few stability indicating methods have been carried for determination of the drug using spectrophotometric method 19,20 , HPLC [21][22][23][24][25] and RP-UPLC-PDA in multidrug combination 26 .…”

Section: Introductionmentioning

confidence: 99%

Green stability indicating UV- spectrophotometric techniques for estimation of tenofovir alafenamide in bulk form and dosage forms

Said

Nasr

Razeq

2021

Azhar International Journal of Pharmaceutical and Medical Scien

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Four simple, precise and sensitive UV-spectrophotometric procedures were achieved for estimation of tenofovir alafenamide in the presence of its alkaline degradate. Dual wavelength was the first one which based on determination of the drug at 235.5 nm and 261.5 nm. First -derivative spectrophotometric method was the second one which the amplitude values were measured at 274 nm using Δλ of 8 nm and a scaling factor of 20. Third one was ratio difference which peak's amplitudes ratio spectra difference of tenofovir alafenamide was measured between 261.5 nm and 252 nm using devisor of 10 μg mL -1 of its alkaline degradate. While the last method was a first derivative of ratio spectra using Δλ = 8 nm and a scaling factor = 10 to measure the amplitude at 275.6 nm. The linearity range was 5-35 μg mL -1 in all procedures. The suggested procedures were effectively utilized for the estimation of the cited drug in bulk form as well as its dosage forms. According to ICH guidelines, all procedures were validated. Moreover, Analytical eco-scale and Green Analytical Procedure Index (GAPI) were used to estimate the greenness of the suggested methods compared with a reported one as two assessment tools.

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Section: Introductionmentioning

confidence: 99%

Green stability indicating UV- spectrophotometric techniques for estimation of tenofovir alafenamide in bulk form and dosage forms

Said

Nasr

Razeq

2021

Azhar International Journal of Pharmaceutical and Medical Scien

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“…A literature survey has revealed that only two articles on UV spectroscopic assay of TAF are reported. A few methods in literature review are found for determination of TAF in combined dosage forms and include High Pressure Liquid Chromatography (HPLC) [1][2][3].A method based on the measurement absorbance of the drug in water at 260nm has been reported and the method obeys Beer's law in the 2-10 μg/ml concentration range [7]. of Tenofovir Alafenamide Fumarate (TAF) in bulk and its tablet dosage form.…”

Section: Introductionmentioning

confidence: 99%

UV-Spectrophotometric Estimation and Forced Degradation Studies of Tenofovir Alafenamide Fumarate (TAF) in its Bulk and Tablet Dosage Form

Bhavyasri¹,

Manisha²,

Rambabu³

2020

IGJPS

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Assessment of the greenness of new stability indicating micellar UPLC and HPTLC methods for determination of tenofovir alafenamide in dosage forms

Said

Nasr

Abdel-Razeq

2021

Journal of Chromatographic Science

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In this study, a green stability indicating chromatographic methods were developed and validated for the quantitative determination of tenofovir alafenamide in the presence of its degradation products in bulk powder as well as in dosage forms. The first method was micellar UPLC in which separation was achieved on kinetex ® 1.7 μm HILIC 100A, LC column using an ecofriendly micellar mobile phase consisting (0.05 M sodium dodecyl sulphate and 0.05 M sodium dihydrogen phosphate, (pH 5.5) and 10% 1-propanol (70:30) at a flow rate of 1 mL min−1 with a UV detection at 210 nm. The second method depended on HPTLC method performed on HPTLC plates pre-coated with silica gel 60 F254 using a mobile phase consisting of n-butanol—acetic acid (7:3, v/v) and detection at 260 nm. Tenofovir alafenamide was subjected to stress conditions including alkaline and acidic degradation. Beer’ law was obeyed over the concentration range of 1–18 μg mL−1 and 0.1–4 μg/spot for micellar UPLC and HPTLC methods, respectively. Both methods are successfully applied to the analysis of the drug in its tablets and validated according to ICH guidelines. In addition, their greenness was assessed using three different tools indicating their least hazardous effect on the environment.

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Spectroscopic Estimation of Tenofovir Alafenamide, an antiretroviral drug

Cited by 6 publications

References 0 publications

Green stability indicating UV- spectrophotometric techniques for estimation of tenofovir alafenamide in bulk form and dosage forms

Green stability indicating UV- spectrophotometric techniques for estimation of tenofovir alafenamide in bulk form and dosage forms

UV-Spectrophotometric Estimation and Forced Degradation Studies of Tenofovir Alafenamide Fumarate (TAF) in its Bulk and Tablet Dosage Form

Assessment of the greenness of new stability indicating micellar UPLC and HPTLC methods for determination of tenofovir alafenamide in dosage forms

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