Spectroscopic and histological evaluation of wound healing progression following Low Level Laser Therapy (LLLT)

Abstract

The present study focuses on the evaluation of the effect of He-Ne laser on tissue regeneration by monitoring collagen synthesis in wound granulation tissues in Swiss albino mice using analysis of laser induced fluorescence (LIF) and light microscopy techniques. The spectral analyses of the wound granulation tissues have indicated a dose dependent increase in collagen levels during the post-wounding days. The histological examinations on the other hand have also shown a significant increase in collagen deposition along with the reduced edema, leukocytes, increased granulation tissue, and fibroblast number in the optimal laser dose treated group compared to the non-illuminated controls.

The Effect of Low-Level Laser on Postoperative Pain After Tibial Fracture Surgery: A Single-Blind Controlled Randomized Clinical Trial

“…This finding is consistent with the findings of other researchers who reported that LLLT could decrease pain during and after the surgery and had a positive effect on wound healing and edema (12). LLLT is used in muscular fatigue (24), wound healing, and pain reduction in dental procedures in patients with and without diabetes (25-27).…”

“…These nonthermal effects are thought to be mediated by a photochemical reaction that alters cell membrane permeability, leading to increased mRNA synthesis and cell proliferation. FDA has started different investigations on LLLT for 15 years and has approved the use of LLLT for pain relief in carpal tunnel syndrome since 2002 (11, 12). It is also used to treat damages in sport injuries and musculoskeletal disorders.…”

Effects of 660 nm low-level laser therapy on muscle healing process after cryolesion

“…Despite the stimulatory effects of laser on tissue repair, investigators have applied it at a wide variety of doses, which makes it difficult to compare published results and determine the best laser parameters for evoking the best tissue response [15][16][17][18]. Therefore, it is important to examine the effects of different fluences of LLLT to define its safety and efficiency.…”

Biomedical Optics and Lasers

“…It has been demonstrated that laser energy at low doses has a positive effect on tissue www.intechopen.com regeneration by increased biomolecule synthesis, formation of connective tissue and increased blood circulation via generation of new vessels [76]. By the way it stimulates tissue healing while helping resolution of inflammation and pain [16].…”