Spectroscopic analysis of polypeptide conformation in polymethyl glutamate monolayers

Loeb, George I.; Baier, Robert E.

doi:10.1016/0021-9797(68)90007-6

Cited by 99 publications

(36 citation statements)

References 9 publications

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“…The possibility of obtaining of polypepfides in different macromolecular conformations has been demonstrated in previous papers [1]. In particular, the existence of monolayers of poly-y-methyl-L-glutamate (I~MG) and of poly-L-alanine (PLA)in the ce-helix conformation when the spreading solvent is chloroform not containing pyridine has been demonstrated [2].…”

Section: Introductionmentioning

confidence: 94%

Mixed monolayers of polypeptides

Gabrielli

Puggelli

Dei

et al. 1988

Colloid & Polymer Sci

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Mixed monolayers of two polypeptides, poly-F-methyl-L-glutamate and poly-L-alanine, in the same ce-helix surface conformation, were studied at the water/air interface.From a comparison of the experimental n-A isotherms with Hugglns' theory and from the examination of the MIR spectra for the two components and their mixtures, it was possible to deduce that poly-y-methyl-L-glutamate and poly-L-alanine were totally miscible at the surface with prevalently attractive interactions and that the mixtures maintained the conformation of the two components.The low values of the interaction parameters, deduced by applying Joos' theory to the collapse pressures of the mixtures, demonstrated that the interactions were prevalently hydrophobic.

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Section: Introductionmentioning

confidence: 94%

Mixed monolayers of polypeptides

Gabrielli

Puggelli

Dei

et al. 1988

Colloid & Polymer Sci

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“…The understanding of the physical chemistry of such monolayers is mainly derived from studies of hydrophobic ester derivatives of poly-L-glutamate and poly-L-aspartate, e.g., poly-γ -methyl-Lglutamate and poly-γ -L-benzyl-L-aspartate (1)(2)(3)(4)(5)(6)(7)(8)(9). In the general case, the compression isotherms of α-helical peptide films display a steep rise, followed by a more or less horizontal plateau.…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic Homopolymers of Native α-L-Amino Acids at the Air–Water Interface: A Study by Circular Dichroism Spectroscopy, Atomic Force Microscopy, and Surface Balance Experiments

Ulvenlund

Gillgren²,

Stenstam³

et al. 2001

Journal of Colloid and Interface Science

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“…[10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] These rigid rodlike molecules lie down flat on the water surface with 2D nematiclike structures. As such they differ from the aforementioned more conventional amphiphilic LMs and liquid crystal films in that the director n, or principal axis of the molecules, lies within the monolayer plane rather than pointing away from it.…”

Section: Introductionmentioning

confidence: 99%

Structure of poly(γ-benzyl-L-glutamate) monolayers at the gas–water interface: A Brewster angle microscopy and x-ray scattering study

Fukuto

Heilmann

Pershan

et al. 1999

The Journal of Chemical Physics

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. 1999. Structure of poly(\(\gamma\)-benzyl-L-glutamate) monolayers at the gas-water interface: A Brewster angle microscopy and x-ray scattering study. Journal of Chemical Physics 111 (21) This paper reports Brewster angle microscopy ͑BAM͒, x-ray specular reflectivity ͑XR͒, grazing incidence diffraction ͑GID͒ and off-specular diffuse scattering ͑XOSDS͒ measurements of Langmuir monolayers formed on water by both mono-and polydisperse samples of ␣-helical poly͑␥-benzyl L-glutamate͒ ͑PBLG͒ as a function of area/monomer A. The microscopic behavior does not exhibit any discernible effects due to differing dispersity. At low surface densities ͑A Ͼϳ21 Å 2 /monomer, surface pressure ⌸ϭ0͒, BAM images reveal partial surface coverage by solidlike monolayer islands. GID measurements show an interhelix peak corresponding to a local parallel alignment of rodlike PBLG molecules, indicating their tendency to aggregate laterally without external pressure. Compression to AϽ21 Å 2 /monomer first leads to full and uniform surface coverage by the monolayer, followed by a steep rise in ⌸ that is accompanied by a decrease in the interhelix distance. Further compression results in a plateau of constant ⌸ in the ⌸-A isotherm ͑ϳ11.5ϽAϽϳ18.5 Å 2 /monomer, ⌸ϳ9 dyn/cm͒, which has previously been attributed to a first-order monolayer-bilayer transition. The interfacial electron density profiles determined by the XR measurements on both sides of the coexistence plateau provide direct evidence for this transition. On the basis of x-ray scattering results, the film on the high-density side of the plateau is shown to consist of a newly formed incomplete and incommensurate second layer that sits on top of and has lower average density than a homogeneous first layer. GID measurements indicate that the second layer can be characterized by larger interhelix d-spacing than the first layer, while XOSDS measurements on the bilayer suggest that the second layer is microscopically inhomogeneous. For both mono-and bilayers, the analysis of observed GID peak widths indicates that the extent of lateral positional correlations between parallel PBLG rods ranges from a few to no more than ϳ15 interhelix distances, implying short-range order.

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Spectroscopic analysis of polypeptide conformation in polymethyl glutamate monolayers

Cited by 99 publications

References 9 publications

Mixed monolayers of polypeptides

Mixed monolayers of polypeptides

Hydrophobic Homopolymers of Native α-L-Amino Acids at the Air–Water Interface: A Study by Circular Dichroism Spectroscopy, Atomic Force Microscopy, and Surface Balance Experiments

Structure of poly(γ-benzyl-L-glutamate) monolayers at the gas–water interface: A Brewster angle microscopy and x-ray scattering study

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