Spectrophotometric Determination of Trypsin and Trypsin Inhibitors

Rhodes, Marvin B.; Hill, Robert M.; Feeney, Robert E.

doi:10.1021/ac60123a014

Cited by 65 publications

(15 citation statements)

References 10 publications

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“…In the present case, the assumption that effective hard sphere radii (or specific volumes) of each species are independent of composition is unrealistic, because at the pH of our experiments, all three proteins bear significant negative charge, 22,23 while TMAO is nearly neutral. 2 The effective radius of the hard particle representing TMAO ( r 2 ) is presumed to be unaffected by electrostatic interactions and does not depend upon whether it is interacting with itself or with protein.…”

Section: Materials and Experimental Methodsmentioning

confidence: 84%

Effect of Nonadditive Repulsive Intermolecular Interactions on the Light Scattering of Concentrated Protein−Osmolyte Mixtures

Fernández

Minton

2010

J. Phys. Chem. B

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The static light scattering of three globular proteins, bovine serum albumin, ovalbumin, and ovomucoid, and binary mixtures of each protein and trimethylamine oxide (TMAO) containing between 10 and 70% protein, were measured as a function of total weight per volume concentration up to 100 g/L. The observed dependence of scattering upon concentration may be accounted for quantitatively by an effective hard sphere model incorporating an extension that takes into account the nonadditive nature of the repulsive intermolecular interaction between protein and TMAO.

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Section: Materials and Experimental Methodsmentioning

confidence: 84%

Effect of Nonadditive Repulsive Intermolecular Interactions on the Light Scattering of Concentrated Protein−Osmolyte Mixtures

Fernández

Minton

2010

J. Phys. Chem. B

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“…Spectrophotometric determination , sensitive amperometric , and fluorimetric assays have been reported for trypsin activity assay and inhibitors screening. Gao et al.…”

Section: Introductionmentioning

confidence: 99%

Trypsin inhibitor screening in traditional Chinese medicine by using an immobilized enzyme microreactor in capillary and molecular docking study

Cheng

Chen

2017

J of Separation Science

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A trypsin immobilized enzyme microreactor was successfully prepared in capillary for studying enzyme kinetics of trypsin and online screening of trypsin inhibitors from traditional Chinese medicine through capillary electrophoresis. Trypsin was immobilized on the inner wall at the inlet of the capillary treated with polydopamine. The rest of the capillary was used as a separation channel. The parameters including the separation efficiency and the activity of immobilized trypsin were comprehensively evaluated. Under the optimal conditions, online screening of trypsin inhibitors each time can be carried out within 6 min. The Michaelis-Menten constant of immobilized trypsin was calculated to be 0.50 mM, which indicated high affinity of the immobilized trypsin for the substrate. The half-maximal inhibitory concentration of known inhibitor of benzamidine hydrochloride hydrate as a model inhibitor was 13.32 mM. The proposed method was successfully applied to screen trypsin inhibitors from 15 compounds of traditional Chinese medicine. It has been found that baicalin showed inhibitory potency. Molecular docking study well supported the experimental result by exhibiting molecular interaction between enzyme and inhibitors.

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“…Trypsin inhibiting activity of faba beans was determined spectrophotometrically using ptoluenesulfonylarginine methyl ester as the synthetic substrate (Rhodes et al 1957(Rhodes et al , 1960 …”

Section: Analysesmentioning

confidence: 99%

Growth Responses of Chicks and Rats Fed Diets Containing Four Cultivars of Raw or Autoclaved Faba Beans

Marquardt¹,

Campbell²,

Stothers³

et al. 1974

Can. J. Anim. Sci.

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Spectrophotometric Determination of Trypsin and Trypsin Inhibitors

Cited by 65 publications

References 10 publications

Effect of Nonadditive Repulsive Intermolecular Interactions on the Light Scattering of Concentrated Protein−Osmolyte Mixtures

Effect of Nonadditive Repulsive Intermolecular Interactions on the Light Scattering of Concentrated Protein−Osmolyte Mixtures

Trypsin inhibitor screening in traditional Chinese medicine by using an immobilized enzyme microreactor in capillary and molecular docking study

Growth Responses of Chicks and Rats Fed Diets Containing Four Cultivars of Raw or Autoclaved Faba Beans

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