Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

Abstract

Biancaea sappan (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl3. The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

Phenolic content and antioxidant activity of formulated biscuits with banana, tempeh and moringa flours

“…The methanolic extract of biscuit samples was analyzed for TFC using the aluminium chloride method. The reaction of aluminium chloride and flavonoids (specifically the C-4 keto groups and C-3 or C-5 hydroxyl group of flavones and flavonols), produces yellow acid stable complex (Kumar et al, 2013;Nurlinda et al, 2021). TFC analysis was conducted based on Aminah et al ( 2017) with slight modifications.…”

Kajian Aktivitas Antioksidan Dari Kayu Secang (Caesalpinia Sappan L.)

“…Kayu secang sering dimanfaatkan untuk obat luka, penawar racun, aktivator sirkulasi darah, desinfektan, antibakteri, antivirus, antiseptik, antiradang, antioksidan, antitumor, dan antikanker. (Azliani & Nurhayati, 2018;Irawan et al, 2022;Nurlinda et al, 2021;Olanwanit & Rojanakorn, 2019;Rina, 2013;R. Sari & Suhartati, 2016).…”