Spectrophotometric determination of secondary amines

Karweik, Dale H.; Meyers, Carl H.

doi:10.1021/ac50038a046

Cited by 24 publications

(8 citation statements)

References 7 publications

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“…Reasonable quantitation was finally achieved by modifying the copper dithiocarbamate method (Karwelk & Meyers, 1979) and by using HPLC. No interference by methylamine (1 mM), methyl isocyanide, dithionite, or any other components of the enzyme assay mixture was evident.…”

Section: Methodsmentioning

confidence: 99%

Nitrogenase reactivity: methyl isocyanide as substrate and inhibitor

Rubinson¹,

Corbin²,

Burgess³

1983

Biochemistry

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We have examined the interaction of methyl isocyanide with the purified component proteins of Azotobacter vinelandii nitrogenase (Av1 and Av2). CH3NC was shown to be a potent reversible inhibitor (Ki = 158 microM) of total electron flow, apparently uncoupling magnesium adenosine 5'-triphosphate hydrolysis from electron transfer to substrate. CH3NC is a substrate (Km = 0.688 mM at Av2/Av1 = 8), and extrapolation of the data indicates that at high enough CH3NC concentration, H2 evolution can be eliminated. The products are methane plus methylamine (six electrons) and dimethylamine (four electrons). There is an excess (relative to methane) of methylamine formed, which may arise by hydrolysis of a two-electron intermediate. A rapid high-performance liquid chromatography/fluorescence method was developed for methylamine determination. The products C2H4 and C2H6 appear to be formed via a reduction followed by an insertion mechanism. CH3NC appears to be reduced at an enzyme state more oxidized than the one responsible for H2 evolution or N2 reduction. Other substrates (C2H2 greater than N2 congruent to azide greater than N2O) all both relieve CH3NC inhibition and inhibit CH3NC reduction. Both effects occur in the same relative order, implying productive (substrate) and nonproductive (inhibitor) modes of binding of CH3NC to the same site.

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Section: Methodsmentioning

confidence: 99%

Nitrogenase reactivity: methyl isocyanide as substrate and inhibitor

Rubinson¹,

Corbin²,

Burgess³

1983

Biochemistry

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“…The IFC can be constructed in two days and occupies a space of only 4X5X6 inches, making it highly portable. (6) Wide Applicability. The IFC has been successfully applied to our mass spectrometric vaporization studies for over a year.…”

Section: Discussionmentioning

confidence: 99%

“…The concentration of the extracted complex is determined spectrophotometrically. Recently an improved version of the methods of Dowden (4) and Umbreit (5) was reported in which ammonia was used as the base and chloroform as the extracting solvent (6). Further examination of 0003-2700/80/0352-1387S01.00/0 this method indicated that with some samples the color formation was not reproducible and that the color faded with time in less than a predictable manner.…”

Section: R'/mentioning

confidence: 99%

“…All solutions were prepared from water which had been doubly distilled from alkaline permanganate. Stock solutions were prepared as previously described (6), with the exception of the dibutylamine which was freshly distilled before the stock solution was prepared. Chloroform was purified for the ethanol interference study to remove the stabilizer (~0.75% ethanol) by conventional methods (7).…”

Section: R'/mentioning

confidence: 99%

“…Procedure. The basic procedure used has been previously reported (6) with the following addition. The pH of the aqueous layer was adjusted within a range of 7.0 to 9.3 by dropwise addition of 25% (v/v) acetic acid.…”

Section: R'/mentioning

confidence: 99%

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Stability of extracted bis(dibutyldithiocarbamate)copper(II) complexes

Tung¹,

Karweik²

1980

Anal. Chem.

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Selected Aliphatic and Aromatic Nitrogen Compounds

Ziegler‐Skylakakis

2023

Patty's Toxicology

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This is an update of a previous chapter. It presents an evaluation of the toxicological potential of a number of aliphatic and aromatic nitrogen compounds, with information on their use, production, and exposure scenarios. These compounds were originally selected on the basis of their use in industry. In general, there has been little new information published in the literature that has significantly altered the previous profile of each compound. The three‐membered ring compounds discussed are ethyleneimine, propylenenimine, and triethylenemelamine. They are alkylating agents and have toxicological properties similar to nitrogen mustards. Six nitrogen mustards and their hydrochlorides are discussed as a group of chemicals. Furthermore, data on representatives of five‐membered ring nitrogen compounds (pyrrolidine, N ‐methyl‐2‐pyrrolidone) and of six‐membered ring compounds (piperidine, piperazine, morpholine, and hexamethylenetetramine) are included. Captan, captafol, and folpet—three N ‐sulfenyl phthalimide fungicides—and benomyl, carbendazim, and thiophanate‐methyl—benzimidazole fungicides—are evaluated as separate groups. Data on 1‐ H ‐benzotriazol are also included in this chapter. The subchapter of each compound or group of compounds includes the physical and chemical properties, information as to use and production, data on the toxicological potential, and standards, regulations, or guidelines of exposure. In addition, data on the environmental impact of each compound or group of compounds are presented and evaluated.

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Spectrophotometric determination of secondary amines

Cited by 24 publications

References 7 publications

Nitrogenase reactivity: methyl isocyanide as substrate and inhibitor

Nitrogenase reactivity: methyl isocyanide as substrate and inhibitor

Stability of extracted bis(dibutyldithiocarbamate)copper(II) complexes

Selected Aliphatic and Aromatic Nitrogen Compounds

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