Spectrophotometric determination of saccharin in different materials by a solvent extraction method using nile blue as reagent

Hernández‐Córdoba, Manuel; López-Garcı́a, Ignacio

doi:10.1016/0039-9140(85)80088-6

Cited by 13 publications

(2 citation statements)

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“…Molecular ultraviolet/visible absorption methods are perhaps the most widely used of all quantitative analysis techniques in chemical and clinical laboratories throughout the world (21), owing mainly to its simplicity, often demanding low-cost equipment. As already mentioned, spectrophotometric methods of saccharin determination have been reported including reaction with azure B (16), Nile-blue (17), astrazone pink FG (18), iodine (19), and phenothazine (20). These methods are often time-consuming and tedious or require drastic reaction conditions.…”

Section: Introductionmentioning

confidence: 99%

“…To fulfill the requirements of quality control, several analytical methods have been described to determine saccharin. Included among these are polarography ( , ), gravimetry (), derivative ultraviolet spectrophotometry (), high-performance liquid chromatography (HPLC) ( 3 , − , potentiometry ( − ), and visible spectrophotometry ( − ). However, most of these methods are time-consuming or require expensive equipment and, consequently, are not ideal for routine analysis.…”

Section: Introductionmentioning

confidence: 99%

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A Simplified Spectrophotometric Method for Routine Analysis of Saccharin in Commercial Noncaloric Sweeteners

Weinert

Pezza

Oliveira

et al. 2004

J. Agric. Food Chem.

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A simple, rapid, and sensitive spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners is proposed. This method is based on the reaction of saccharin with tetrachloro-p-benzoquinone (p-chloranil) accelerated by hydrogen peroxide and conducted in an ethanol:acetone (4:1) medium, producing a violet-red compound (lambda(max) = 550 nm). Beer's law is obeyed in a concentration range of 2.05 x 10(-4) to 3.00 x 10(-3) M with an excellent correlation coefficient (r = 0.9998). The detection limit was 1.55 x 10(-5) M, and the effect of interferences on the spectrophotometric measurements was evaluated. The proposed procedure was applied successfully to the determination of saccharin in noncaloric sweeteners. Recoveries were within 99.2-104.3% with standard deviations ranging from to 0.5-1.6%. Results of the proposed method compare very favorably with those given by the high-performance liquid chromatography method recommended by the Food and Drug Administration.

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Section: Introductionmentioning

confidence: 99%