A Simplified Spectrophotometric Method for Routine Analysis of Saccharin in Commercial Noncaloric Sweeteners

Abstract

A simple, rapid, and sensitive spectrophotometric method for routine analysis of saccharin in commercial noncaloric sweeteners is proposed. This method is based on the reaction of saccharin with tetrachloro-p-benzoquinone (p-chloranil) accelerated by hydrogen peroxide and conducted in an ethanol:acetone (4:1) medium, producing a violet-red compound (lambda(max) = 550 nm). Beer's law is obeyed in a concentration range of 2.05 x 10(-4) to 3.00 x 10(-3) M with an excellent correlation coefficient (r = 0.9998). The detection limit was 1.55 x 10(-5) M, and the effect of interferences on the spectrophotometric measurements was evaluated. The proposed procedure was applied successfully to the determination of saccharin in noncaloric sweeteners. Recoveries were within 99.2-104.3% with standard deviations ranging from to 0.5-1.6%. Results of the proposed method compare very favorably with those given by the high-performance liquid chromatography method recommended by the Food and Drug Administration.

A simple spectrophotometric method for the determination of methyldopa using p-chloranil in the presence of hydrogen peroxide

“…To the best of our knowledge, there is a single spectrophotometric method for the determination of saccharin using p-cloranil described in the literature that also used H 2 O 2 as accelerator agent [37]. So, our results corroborate with those already described for analysis of saccharin, suggesting that reactions involving p-chloranil and electrons donors could be accelerated by using of H 2 O 2 .…”

Determinação espectrofotométrica de citrato de sildenafil em formulações farmacêuticas

“…No melhor do nosso conhecimento, há somente um método descrito na literatura que envolve o uso de peróxido de hidrogênio como acelerador da reação de transferência de carga com p-cloranil, o qual foi desenvolvido em nosso laboratório e aplicado na determinação de sacarina. 22 Um estudo inicial foi feito para selecionar o meio reacional mais adequado para o desenvolvimento da reação na presença de peróxido de hidrogênio. Foram testados acetona, acetonitrila e dioxano como solvente para a dissolução do p-cloranil e metanol, acetona, acetonitrila e dioxano para ajuste do volume dos balões.…”

Flow-Injection Spectrophotometric Determination of Azithromycin in Pharmaceutical Formulations Using p-Chloranil in the Presence of Hydrogen Peroxide

“…33,34 Spectrophotometric methods based on these interactions are usually simple and convenient because of the rapid formation of complexes. [34][35][36][37] Azithromycin (electron donor) reacts instantaneously with p-chloranil (p-acceptor) in the presence of hydrogen peroxide, resulting in the formation of an intense purple-red product at room temperature, which exhibits an absorption maximum at 540 nm. This reaction is markedly accelerated by hydrogen peroxide; however, without hydrogen peroxide, the reaction is very slow at room temperature.…”