Spectrophotometric Determination of Paracetamol by Reduction of 18-Molybdo-2-Phosphate Heteropoly Anion

Abstract

A very simple, rapid and sensitive spectrophotometric method for paracetamol assay in pharmaceuticals based on the reduction of 18-molybdo-2-phosphate heteropoly complex was developed. Reaction of 18-molybdo-2-phosphate with paracetamol is very fast at room temperature. The stoichiometry of the reaction is 1:1. The molar absorptivity calculated for paracetamol was equal to that for heteropoly blue formed in the studied reaction and was of 1.15×10 4 l mol-1 cm-1 at 820 nm. Beer`s law was followed for paracetamol concentrations from 0.3 to 7.5 µg ml-1 (l = 1 cm). The detection limit of the method was 0.03 µg ml-1 (l = 5 cm), and a relative standard deviation did not exceed 2.5 %. The method was successfully applied to the determination of paracetamol in pharmaceuticals.

Validated HPLC and stability-indicating densitometric chromatographic methods for simultaneous determination of camylofin dihydrochloride and paracetamol in their binary mixture

“…Paracetamol (N-acetyl-para-amino-phenol) has antipyretic and analgesic action and weak anti-inflammatory effect [1]. Many techniques have been used for its determination, including spectrophotometry [15][16][17], HPLC [18][19][20][21] TLC [22][23][24][25], GC [26][27][28][29], fluorometry [30][31][32] and electrochemistry [33][34][35][36].…”