Spectrophotometric determination of microgram amounts of adrenaline with chloranil

Al-Abachi, Mouayed Q.; Al-Ghabsha, Thabit S.; Shahbaz, Nazli A.

doi:10.1016/0026-265x(85)90113-4

Cited by 13 publications

(7 citation statements)

References 7 publications

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“…With the production of •OH via a Fenton reaction, the thioketal bonds in the nanocomplexes can be broken efficiently by the produced •OH and release the dopamine groups. We used tetrachlorobenzoquinone (TCBQ) to test the release of dopamine . The maximum absorption wavelength of the complex between TCBQ and dopamine was 480 nm by UV–vis absorbance spectra.…”

Section: Resultsmentioning

confidence: 99%

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Protein-Delivering Nanocomplexes with Fenton Reaction-Triggered Cargo Release to Boost Cancer Immunotherapy

Zhou

Japir

et al. 2022

ACS Nano

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Immunotherapeutic efficacy of tumors based on immune checkpoint blockade (ICB) therapy is frequently limited by an immunosuppressive tumor microenvironment and cross-reactivity with normal tissues. Herein, we develop reactive oxygen species (ROS)-responsive nanocomplexes with the function of ROS production for delivery and triggered release of anti-mouse programmed death ligand 1 antibody (αPDL1) and glucose oxidase (GOx). GOx and αPDL1 were complexed with oligomerized (−)-epigallocatechin-3-O-gallate (OEGCG), which was followed by chelation with Fe 3+ and coverage of the ROS-responsive block copolymer, POEGMA-b-PTKDOPA, consisting of poly(oligo(ethylene glycol)methacrylate) (POEGMA) and the block with thioketal bond-linked dopamine moieties (PTKDOPA) as the side chains. After intravenous injection, the nanocomplexes show prolonged circulation in the bloodstream with a half-life of 8.72 h and efficient tumor accumulation. At the tumor sites, GOx inside the nanocomplexes can produce H 2 O 2 via oxidation of glucose for Fenton reaction to generate hydroxyl radicals (•OH) which further trigger the release of the protein cargos through ROSresponsive cleavage of thioketal bonds. The released GOx improves the production efficiency of •OH to kill cancer cells for release of tumor-associated antigens via chemodynamic therapy (CDT). The enhanced immunogenic cell death (ICD) can activate the immunosuppressive tumor microenvironment and improve the immunotherapy effect of the released αPDL1, which significantly suppresses primary and metastatic tumors. Thus, the nanocomplexes with Fenton reaction-triggered protein release show great potentials to improve the immunotherapeutic efficacy of ICB via combination with CDT.

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Section: Resultsmentioning

confidence: 99%

“…We used tetrachlorobenzoquinone (TCBQ) to test the release of dopamine. 64 The maximum absorption wavelength of the complex between TCBQ and dopamine was 480 nm by UV− vis absorbance spectra. The amount of the released dopamine was determined by the standard curve (Figure S12).…”

Section: Resultsmentioning

confidence: 99%

Protein-Delivering Nanocomplexes with Fenton Reaction-Triggered Cargo Release to Boost Cancer Immunotherapy

Zhou

Japir

et al. 2022

ACS Nano

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“…В літературі описано СФ метод аналізу АД за використання різних хромогенів. Перші спроби колориметричного визначення АД обмежувались реакцією взаємодії його з 1 мМ хлораміном в лужному середовищі при нагріванні (60 °С) та вимірюванні кінцевого кольорового продукту при 350 нм (Al-Abachi et al, 1989). Діапазон лінійності такого методу становив 0,4-28 мг/мл.…”

Section: обговоренняunclassified

A new spectrophotometric method analysis of adrenaline in pharmaceuticals based on laccase-like nanozymes

et al. 2022

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Nanozymes, which have high enzyme-like activity of natural enzymes, are very promising for analytical purposes, in particular, for the development of methods for sensitive, quantitative detection of practically important analytes – biomarkers of common diseases or pharmaceutical products. Recently, it has been reported that artificial enzymes with laccase-like activity or “nanolaccases (nLacs),” can serve as catalytic elements for the creation of sensitive methods for catecholamines. Our work aimed to obtain laccase-like nanozymes and characterize and demonstrate their suitability for spectrophotometric adrenaline (AD) analysis. In this article, we report on preparing five hexacyanoferrate nanoparticles (HCF NPs) that possess laccase-like activity, particularly, Co-HCF, Ni-HCF, Mn-HCF, Zn-HCF, and Cu-HCF. Among the investigated nLacs, Cu-HCF was selected and characterized. It was shown that Cu-HCF reveals the highest activities, is stable in various pH conditions in the range 3.0–6.5, and has satisfactory stored stability. A new spectrophotometric method for the quantitative detection of AD was created using the selected nLacs. The linearity of the proposed method is in the range from 5 μM to 50 μM (0.66–11 μg/ml), and the limit of detection is 1.5 μM (0.33 μg/ml), which is lower than that catalyzed by native laccase (1.15 μg/ml). The proposed method was tested on the real samples of pharmaceuticals, and the obtained data agree with the data declared by the producer. The resulting nLacs have great potential for use in catalysis of mimetics, environmental restoration, and sensor design. Thus methods, the obtained Cu-HCF has great potential application in spectrophotometric and biosensor method for analysis of biologically active toxic compounds in surface waters.

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“…The charge transfer complex reaction has been used for determination of catecholamine by using various organic reagents such as chloranil (27) , DDQ (28) and fluoranil (29) . The present paper describe simple, sensitive, and accurate spectrophotometric determination of dopamine hydrochloride, methyl dopa and L-dopa using charge transfer complex formation reaction in aqueous medium with bromanil in the presences of solution of pH 9 borate buffer.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Catecholamines in Pharmaceutical Preparations Via Charge Transfer Complex Formation Using Bromanil Reagent

Al-Sharook¹

2007

J.Edu.Sci.

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Spectrophotometric determination of microgram amounts of adrenaline with chloranil

Cited by 13 publications

References 7 publications

Protein-Delivering Nanocomplexes with Fenton Reaction-Triggered Cargo Release to Boost Cancer Immunotherapy

Protein-Delivering Nanocomplexes with Fenton Reaction-Triggered Cargo Release to Boost Cancer Immunotherapy

A new spectrophotometric method analysis of adrenaline in pharmaceuticals based on laccase-like nanozymes

Spectrophotometric Determination of Catecholamines in Pharmaceutical Preparations Via Charge Transfer Complex Formation Using Bromanil Reagent

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