Spectrophotometric Determination of Iron(II) after Solid Phase Extraction of Its 2,2′ Bipyridine Complex on Silica Gel-Polyethylene Glycol

Pourreza, Nahid; Rastegarzadeh, Saadat; Kiasat, Ali Reza; Yahyavi, Hossein

doi:10.1155/2013/548345

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…One of the major advantages of the new method is the small amount of sample needed. In case Fe concentrations were below the detection limit of this method and more sample were not available, extracts could be concentrated following the procedure proposed by Pourreza et al (). Anthocyans, if present in large amounts, potentially interfere with the dipyridyl method ( Ueda et al, ).…”

Section: Discussionmentioning

confidence: 99%

Micro‐method to determine iron concentrations in plant tissues using 2,2′ bipyridine

Hartmann

Asch

2018

J. Plant Nutr. Soil Sci.

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Research often needs to determine iron (Fe) concentrations in plant tissue samples. Current established methods depend on equipment and often require skilled laboratory staff, large sample sizes, and are relatively slow and expensive. We propose an efficient and fast method for measuring Fe concentrations of small rice samples via a microplate reader using sodium dithionite (SDT) as reducing agent and dipyridyl (DPD) as coloring agent. The results show that the method yields results comparable to ICP‐OES measurements which were used as standard method. Different concentrations of the chemicals used were tested for extraction, reducing power, and coloring efficiency, to optimize the method for the range of concentrations to be expected in rice under toxic Fe conditions. Best results were obtained using 500 mM SDT and 10 mM DPD, a sample size of 0.01g dry weight, and Fast Prep as extraction method. A linear calibration curve was obtained for 0 to 100 mg kg−1 iron within the measured samples. The method proposed here was successfully applied to measure total Fe concentration in oven‐dried, milled plant samples. Applicability of the method for tissues other than rice and suboptimal extraction methods are discussed.

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Section: Discussionmentioning

confidence: 99%

Micro‐method to determine iron concentrations in plant tissues using 2,2′ bipyridine

Hartmann

Asch

2018

J. Plant Nutr. Soil Sci.

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“…At present, some determination methods of iron(II) and GA respectively have been reported. For example, the methods used to detect iron(II) include chemiluminescence, [7,8] liquid chromatography, [9] colorimetry, [3,10] spectrophotometric, [11][12][13] flame atomic absorption spectrometry, [14] photothermal effect, [15] fluorescence analysis [16][17][18] and so on. Analysis and determination methods of GA include flow injection chemiluminescent method, [19] spectrophotometric, [20][21][22] isocratic column liquid chromatography, [23] differential pulse polarography, [24] colorimetry, [6,25] electrochemical method based on molecularly imprinted polymers, [26] fluorescence, [27,28] voltammetric method, [29][30][31] etc.…”

Section: Introductionmentioning

confidence: 99%

A dual‐mode optical assay for iron(II) and gallic acid based on Fenton reaction

Ding

et al. 2022

Luminescence

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The hydroxyl radicals (·OH) produced by the Fenton reaction of iron(II) and hydrogen peroxide (H2O2) can oxidize the colorless 3,3′,5,5′‐tetramethylbenzidine (TMB) to blue oxidized TMB (Ox‐TMB), resulting in a decrease in the fluorescence intensity of the reaction system and an increase in ultraviolet absorption. Ox‐TMB had a visible absorption peak at 625 nm and a fluorescence peak around 420 nm. When gallic acid (GA) was added to the system, Ox‐TMB was reduced to TMB, which made the color of the system disappear and the fluorescence recover. The linear ranges for determination of iron(II) were 0.5–10 μM (fluorometric) and 0.5–20 μM (colorimetric), and the detection limits were 0.25 μM (fluorometric) and 0.28 μM (colorimetric). The linear ranges for determination of GA were 0–80 μM (fluorometric) and 0–60 μM (colorimetric), and the detection limits were 0.31 μM (fluorometric) and 0.8 μM (colorimetric). The results of anti‐interference experiments shew that this dual‐mode assay had very good selectivity for the determination of iron(II) and GA.

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“…For determining the iron concentration in aqueous solution, several colorimetric methods have been reported with using thiocyanate ion, [3][4][5][6] 1,10-phenanthroline (phen), [7][8][9][10][11] 2,2′-bipyridine (bpy, Fig. 1) 12,13 and so on. Thiocyanate ion forms a stable bloody red-colored complex with Fe(III), and the complex is used for the quantification of Fe(III).…”

Section: Introductionmentioning

confidence: 99%

Mutual Separation of Fe(II) and Fe(III) Using Cyclohexane/Water/ Ionic-liquid Triphasic Extraction System with 2,2′-Bipyridine and Tri-n-octylphosphine Oxide

2020

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The triphasic extraction system with two extract phases can be used to separate two materials simultaneously into each phase. In this article, a possible mutual separation of Fe(II) and Fe(III) was studied using the cyclohexane/water/ionic liquid (IL) triphasic extraction system for Fe speciation. For Fe(II) and Fe(III) extraction, 2,2′-bipyridine (bpy) and tri-noctylphosphine oxide (TOPO) were selected as extractants, respectively. It was suggested that [Fe II (bpy)3] 2+ and Fe III (TOPO)4 3+ •3Tf2Nwere extracted into the IL (1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide, C4mimTf2N) phase and the cyclohexane phase, respectively, and both of the extractants also acted as masking agents. On simultaneous separation using the triphasic system, Fe(II) and Fe(III) were quantitatively extracted into the IL phase and the cyclohexane phase, respectively, and their mutual separation was achieved.

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Spectrophotometric Determination of Iron(II) after Solid Phase Extraction of Its 2,2′ Bipyridine Complex on Silica Gel-Polyethylene Glycol

Cited by 9 publications

References 20 publications

Micro‐method to determine iron concentrations in plant tissues using 2,2′ bipyridine

Micro‐method to determine iron concentrations in plant tissues using 2,2′ bipyridine

A dual‐mode optical assay for iron(II) and gallic acid based on Fenton reaction

Mutual Separation of Fe(II) and Fe(III) Using Cyclohexane/Water/ Ionic-liquid Triphasic Extraction System with 2,2′-Bipyridine and Tri-n-octylphosphine Oxide

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