Spectrophotometric determination of gabapentin in pharmaceutical formulations using ninhydrin and π-acceptors

Siddiqui, Farhan Ahmed; Arayne, M. Saeed; Sultana, Najma; Qureshi, Faiza; Mirza, Agha Zeeshan; Zuberi, M. Hashim; Bahadur, S. Asath; Afridi, Nawab Sher; Shamshad, Hina; Rehman, Nadia

doi:10.1016/j.ejmech.2010.02.058

Cited by 49 publications

(31 citation statements)

References 22 publications

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“…[39][40][41][42][43][44][45] Ninhydrin is extensively used to detect compounds of pharmaceutical importance [46][47][48][49][50][51][52][53][54][55][56][57] and in kinetic studies. [58][59] Scheme 1.…”

Section: -10mentioning

confidence: 99%

Ninhydrin in synthesis of heterocyclic compounds

Ziarani¹,

Lashgari²,

Azimian³

et al. 2015

Arkivoc

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Ninhydrin has been utilized in many heterocyclic preparations and considered as an important building block in organic synthesis. There is a wide range of reactions that include ninhydrin in the synthesis of heterocyclic compounds. This review highlights the advances in the use of ninhydrin as starting material in the synthesis of various organic compounds and drugs in a fully comprehensive way, from its first isolation in 1910 to the end of 2013. There is also a diversity of multi-component reactions of ninhydrin and we highlight the recent reports in this review.

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Section: -10mentioning

confidence: 99%

Ninhydrin in synthesis of heterocyclic compounds

Ziarani¹,

Lashgari²,

Azimian³

et al. 2015

Arkivoc

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“…1) The analytical method development for PGB, GBP and VGB determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been proposed [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] and used for their determination in bulk or pharmaceutical dosage forms, plasma, serum, and urine. These methods employed derivatization reagents such as fluorescamine, [2][3][4][17][18][19] 7-chloro-4-nitrobenzofurazan, [5][6][7] 1-fluoro-2,4-dinitrobenzene, 8) o-phtaldialdehyde, 9,10) 2,4,6-trinitrobenzene sulfonic acid, 11,12) and 9-fluorenylmethylchloroformate.…”

Section: Pregabalin ((3s)-3-(aminomethyl)-5-methylhexanoic Acid) (Pgbmentioning

confidence: 99%

“…13) The resulted derivatives were measured using non-separation spectrophotometric 5,6,[15][16][17] and spectrofluorimetric methods. 2,[5][6][7]17,19) Moreover, HPLC 3,[8][9][10][11][12][13][14]18) and capillary electrophoresis (CE) methods 4) using precolumn derivatization with either UV or fluorescent detector were also used.…”

Section: Pregabalin ((3s)-3-(aminomethyl)-5-methylhexanoic Acid) (Pgbmentioning

confidence: 99%

A Simple High-Throughput Method for Determination of Antiepileptic Analogues of γ-Aminobutyric Acid in Pharmaceutical Dosage Forms Using Microplate Fluorescence Reader

Martinc

Vovk

2013

CHEMICAL & PHARMACEUTICAL BULLETIN

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Pregabalin (PGB), gabapentin (GBP), and vigabatrin (VGB) are structural analogues of γ-aminobutyric acid used for the treatment of different forms of epilepsy. Their analytical determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been developed and used for their determination in bulk or pharmaceutical dosage forms. We aimed to develop a highthroughput method using a microplate reader with fluorescence detection and simple derivatization with fluorescamine. Obtained method involves derivatization step of only 5 min at room temperature and simultaneous measurements of 96 samples (λ ex 395, λ em 476 nm) thus rendering excellent high-throughput analysis. The method was found to be linear with r 2 >0.998 across investigated analytical ranges of 0.75 to 30.0 µg/ mL for PGB, 2.00 to 80.0 µg/mL for GBP, and 1.50 to 60.0 µg/mL for VGB. Intraday and interday precision values did not exceed 4.93%. The accuracy was ranging between 96.6 to 103.5%. The method was also found to be specific since used excipients did not interfere with the method. The robustness study showed that derivatization procedure is more robust than spectrofluorimetric conditions. The developed high-throughput method was successfully applied for determination of drug content and dissolution profiles in pharmaceutical dosage forms of studied antiepileptic drugs.

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“…This type of complexation is widely applied since last decade to analyze and characterize many of organic compounds like carboxylic acids [6], amines [7] and so on. These are of great importance for determining a number of parameters like ionization potential [8], dipole moment [9], oscillator's strength [10] and resonance energy [11].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Spectrophotometric Determination Ibuprofen Charge Transfer Complexes with P-Chloranil, 7,7,8,8-Tetracyanoquinodimethane, Bromothymol Blue, Methyl Orange and Picric Acid

M¹

2013

J Bioanal Biomed

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Three simple, sensitive and inexpensive spectrophotometric methods have been described for the assay of ibuprofen in bulk drugs and pharmaceutical formulations. The developed methods are based on the formation of colored charge transfer complexes of ibuprofen with p-chloranil, 7,7,8,8-tetracyanoquinodimethane, bromothymol blue, methyl orange and picric acid in acetonitrile as solvent. These newly formed complexes were found to absorb at 438, 394, 403, 418, 374 nm respectively. Optimizations of various experimental conditions are described. Beer's law obeyed in the concentration range6-54, 2-24, 4-28, 3-21 and 4-28 µgmL -1 with correlation coefficient >0.998 in each case and lower limit of detection values were 76, 90, 234, 63 and 189 ng mL -1 , respectively. The association constants and standard free energy changes were studied using Benesi-Hildebrand plots. Oscillator's strength, ionization potential and energy of complexes in the ground state for all the complexes have been calculated. For further confirmation, solid charge transfer complexes were synthesized and characterized by IR and 1 H-NMR spectroscopy. The applicability of the method was demonstrated by the determination of studied drugs in commercial tablets with satisfactory results. No interference from excipients was observed in the formulations.

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Spectrophotometric determination of gabapentin in pharmaceutical formulations using ninhydrin and π-acceptors

Cited by 49 publications

References 22 publications

Ninhydrin in synthesis of heterocyclic compounds

Ninhydrin in synthesis of heterocyclic compounds

A Simple High-Throughput Method for Determination of Antiepileptic Analogues of γ-Aminobutyric Acid in Pharmaceutical Dosage Forms Using Microplate Fluorescence Reader

Synthesis and Spectrophotometric Determination Ibuprofen Charge Transfer Complexes with P-Chloranil, 7,7,8,8-Tetracyanoquinodimethane, Bromothymol Blue, Methyl Orange and Picric Acid

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