Spectrophotometric determination of fenoterol hydrobromide in pure form and dosage forms

El-Shabrawy, Yasser; Belal, F.; El-Din, M. Sharaf; Shalan, Shereen

doi:10.1016/s0014-827x(03)00186-1

Cited by 15 publications

(7 citation statements)

References 23 publications

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“…In recentr eports. NBD-Clw as further used as a chromogenic reagent for the determinationofsome primary and secondaryamines [12][13][14][15][16]. In the present study, ISX was foundt or eactw ith NBD-Cl in borate buffer of pH 7.8 producing ayellow color with maximum absorbance at 395 nm.…”

Section: Resultsmentioning

confidence: 46%

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Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

2006

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As imple and sensitivek inetic spectrophotometric method was developedf or the determinationo fi soxsuprine in pharmaceutical preparations.T he method is basedupon akinetic investigationofthe coupling reaction between isoxsuprine HCl and4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.8f or af ixedt ime of 30 min. The absorbance of the yellow coloured product was measured at 395 nm. The absorbance-concentrationp lot was rectilinear over the rangeo f2 -20 µg mL -1 (r= 0.9994) with minimum detectability of 0.6 µg mL -1 (1.75 x10 -6 M). The different experimental parameters affecting the development and stability of the colourw ere carefully studied and optimized. The determination of isoxsuprine by the fixed-concentration and rate-constantm ethods is also feasible with the calibratione quations obtained, but the fixed time methodh as been found to be more applicable.T he proposed methodw as further applied to the determination of the drug in formulations. The results obtained were in good agreementw ith thoseo btained usingar eferencem ethod. The reaction pathway wasproposed. KeywordsKineticd etermination,i soxsuprine HCl, (NBD-Cl),p harmaceutical preparations.Scientia Pharmaceutica (Sci. Pharm.) 74, 99-119

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Section: Resultsmentioning

confidence: 46%

“…The latteri sau seful derivatizing agent for primary and secondary amines, thiol, etc. Several pharmaceutical compounds have been determined through this approach [11][12][13][14][15][16].…”

mentioning

confidence: 99%

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

2006

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“…30) In recent reports, NBD-Cl was further used as a chromogenic reagent for the determination of some primary and secondary amines. [14][15][16][17][18][19][20][21][22][23][24] In the present study, DSL was found to react with NBD-Cl in borate buffer of pH 7.6 producing a yellow color with maximum absorbance at 485 nm; Method I. Figure 2a illustrates the absorption spectrum of the reaction product.…”

Section: Resultsmentioning

confidence: 88%

“…14) Moreover, the condensation product with NBD-Cl was found to be also a fluorescent derivative. NBD-Cl has been used as a derivatizing agent for many compounds either per se or in formulations and biological fluids such as lisinopril, 15) gliclazide, 16) tianeptine, 17) ranitidine and nizatidine, 18) atenolol, 19) fenetrol, 20) trimetazidine, 21) and recently befunolol, 22) some b-blockers 23) and paroxetine. 24) Sanger's reagent (DNFB) on the other hand has been applied as a chromophore reactant in the spectrophotometric determination of norfloxacin, 25) enalapril and lisinopril.…”

mentioning

confidence: 99%

Spectrophotometric, Spectrofluorometric and HPLC Determination of Desloratadine in Dosage Forms and Human Plasma

2007

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Four sensitive, simple and specific methods were developed for the determination of desloratadine (DSL), a new antihistaminic drug in pharmaceutical preparations and biological fluids. Methods I and II are based on coupling DSL with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.6 where a yellow colored reaction product was obtained and measured spectrophotometrically at 485 nm (Method I). The same product could be measured spectrofluorometrically at 538 nm after excitation at 480 nm (Method II). Methods III and IV, on the other hand, involved derivatization of DSL with 2,4-dinitrofluorobenzene (DNFB) in borate buffer of pH 9.0 producing a yellow colored product that absorbs maximally at 375 nm (Method III). The same derivative was determined after separation adopting HPLC (Method IV). The separation was performed on a column packed with cyanopropyl bonded stationary phase equilibrated with a mobile phase composed of acetonitrile-water ( (Method IV). The four developed procedures were applied for the determination of DSL in tablets without any interference from the excipients. A low LOQ is a common requirement to support a clinical development program and when this is coupled with a simple procedure for sample preparation and feasible analytical tool, they would be the requirements of an optimized analytical method. Although the previously reported methods that were applied in pharmacokinetic studies of desloratadine possess the required low LOQ, they lack the simple sample preparation and method feasibility that were obtained in our spectrofluorometric method using NBD-Cl as a derivatizing agent where a low LOQ of 0.012 mg/ml was attained which allowed the analytical determination of DSL in spiked and real human plasma. ExperimentalMaterials and Reagents All chemicals were of analytical reagent grade. Desloratadine reference standard was kindly provided by ScheringPlough Corporation, U.S.A. Aerius tablets labeled to contain 5 mg DSL per tablet were purchased from commercial sources at a local pharmacy. Plasma samples were obtained from Mansoura University Hospital, Mansoura, Egypt, and were kept frozen until use after gentle thawing. Sep-Pak ® C18 solid phase extraction cartridges, 200 mg (SPE) were purchased from Waters Corporation (Milford, MA, U.S.A.). 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was purchased from Sigma (St. Louis, MO, U.S.A.). A stock solution containing 2 mg/ml was freshly prepared in methanol. 2,4-Dinitrofluorobenzene (DNFB) was also purchased from Sigma (St. Louis, MO, U.S.A.). A stock solution containing 0.25% (v/v) was freshly prepared in methanol. Borate buffer solutions (0.2 M) were prepared by mixing appropriate volumes of 0.2 M boric acid and 0.2 M NaOH and adjusting the pH to 7.6 and 9.0 using a pH meter.29) Formic acid 85%, acetonitrile and methanol; AR Grade, Aldrich (St. Louis, MO, U.S.A.).Apparatus A Shimadzu UV-Visible 1601 PC spectrophotometer was used for the spectrophotometric measurements (P/N 206-67001). The recording range was 0-1.0.The f...

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“…A detecção na urina por cromatografia gasosa acoplada a espectrometria de massas de vários agonistas adrenérgicos, incluindo o fenoterol, foi descrita por Damasceno et al (2002). El-Shabrawy et al (2003) e Beyene, Staden e Stefan (2004) relataram a determinação espectrofotométrica do BF após derivatização, produzindo compostos coloridos medidos na região do visível.…”

Section: Unitermosunclassified

Determinação espectrofotométrica do bromidrato de fenoterol em preparações farmacêuticas

Zamuner¹,

Carrion²,

Magalhães

2008

Rev. Bras. Cienc. Farm.

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Um método espectrofotométrico simples foi desenvolvido para a determinação do bromidrato de fenoterol (BF) em comprimidos. É um agonista adrenérgico β 2 -seletivo, usado como broncodilatador e tocolítico. As especialidades farmacêu-ticas comercializadas no Brasil, que contém o BF como princípio ativo, estão sob a forma de comprimidos, solução oral (gotas), xarope e aerossol dosificador para inalação. É comercializado, também, em associação com o ibuprofeno, um analgésico e antiinflamatório, derivado dos

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Spectrophotometric determination of fenoterol hydrobromide in pure form and dosage forms

Cited by 15 publications

References 23 publications

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Kinetic Spectrophotometric Determination of Isoxsuprine in Dosage Forms Through Derivatisation with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Spectrophotometric, Spectrofluorometric and HPLC Determination of Desloratadine in Dosage Forms and Human Plasma

Determinação espectrofotométrica do bromidrato de fenoterol em preparações farmacêuticas

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