Spectrophotometric determination of cysteine

Bydalek, T. J.; Poldoski, J.E.

doi:10.1021/ac60268a042

Cited by 14 publications

(5 citation statements)

References 8 publications

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“…It was observed that under the described experimental conditions, the optimum pH range for the reaction was 2.5-4.5. This is markedly different from the 7-8 p H range selected previously for the colorimetric determination of cysteine (31) and thioethylamine (32) by means of nitrilotriacetateferrate(II1).…”

Section: Scheme Icontrasting

confidence: 79%

Colorimetric Determination of Acetylcysteine, Penicillamine, and Mercaptopropionylglycine in Pharmaceutical Dosage Forms

Raggi

Cavrini

Pietra

1982

Journal of Pharmaceutical Sciences

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Section: Scheme Icontrasting

confidence: 79%

Colorimetric Determination of Acetylcysteine, Penicillamine, and Mercaptopropionylglycine in Pharmaceutical Dosage Forms

Raggi

Cavrini

Pietra

1982

Journal of Pharmaceutical Sciences

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“…Thiols were dissolved (0.5 mM) in Balk's medium with amino acid composition of Eagle's basal medium, but lacking phenol red or serum, and incubated in flat T-flasks. Cysteine was measured by the method of Bydalek and Poldoski (11). Color development was slower with cysteamine and 2-mercaptoethanol; optical density readings were made after standing the reactants at room temperature for 20 and 100 min, respectively.…”

Section: Table II Growth Promoting Acti'dty Of Thiols Compared With Cmentioning

confidence: 99%

Promotion of Replication in Lymphoid Cells by Specific Thiols and Disulfides in Vitro

Broome

Jeng

1973

The Journal of Experimental Medicine

211

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Lymphocyte division has been the subject of many studies from the point of view of cellular immunology and antibody synthesis. Blast cell transformation and mitosis occur both in vitro and in vivo under stimulus from a number of agents which act on the cell surface, particularly in experimental situations by lectins (1).Although the sequence of events which connect the mitogenic stimulus to DNA synthesis is still incompletely known and several different cell types may be involved, a nmnber of preliminary changes have been recognized. Thus, for example, the cyclic AMP level in lymphocyte cultures rises within 2 rain of stimulation (2), membrane biosynthesis increases within 1/4 h of stimulation (3), nuclear template activity increases within 2 h (4). I t is of considerable importance to an understanding of the neoplastic change in lymphoid cells to know the extent to which such processes and factors controlling them are retained in replicating lymphoma cells, and the extent to which they are altered.We here report a comparison, between the effects of certain thiols and disulfides which we have found to be necessary for the growth of mouse lymphoma cells in vitro, and their effects in enhancing the action of mitogens on splenic lymphocytes.Our observations stem from the finding that mouse lymphoma cells of the line L1210 (V) which had previously been established in culture only with great difficulty (5), proliferate in a medium formulated by Balk (6). The specific growth promoting component of this medium was found to be its high concentration of added L-cysteine (1.5 mM) (7). As will be described, a number of thiols and disulfides can substitute for L-cysteine, some at very low concentration (to < 1 #M). Precise structure activity relationships exist. Furthermore, 13 of 23 other mouse leukemic and neoplastic lymphoid cell lines were also thiol-disulfide dependent in vitro.

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“…13 Therefore, it is important to develop sensitive method for the determination of L-cysteine. Various methods have been used for the determination of L-cysteine, such as photoelectrochemical detection, 14 spectrometric method, 15,16 chromatographic separation method 17 and electrochemical method. 18 Among these methods, electrochemical method using GCE modified with nanoscale materials has been widely reported to improve the selectivity and sensitivity for the detection of organic acids.…”

Section: Introductionmentioning

confidence: 99%

Growth of Li Doped Bismuth Oxide Nanorods and its Electrochemical Performance for the Determination of L-Cysteine

2017

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Li doped bismuth oxide nanorods have been prepared using sodium bismuthate and Li acetate. X-ray diffraction (XRD) pattern shows that the nanorods are composed of monoclinic Bi 2 O 4 and cubic LiBi 12 O 18.50 phases. Scanning electron microscopy (SEM) observation shows that the nanorods have the length and diameter of 1-5 μm and 50-350 nm, respectively. The formation of the Li doped bismuth oxide nanorods is closely relative to the hydrothermal conditions. The electrochemical performance for the determination of L-cysteine based on a Li doped bismuth oxide nanorods modified glassy carbon electrode (GCE) has been developed. The CV peak current increases obviously and linearly with increasing the scan rate. Under the optimal conditions, Li doped bismuth oxide nanorods modified GCE exhibits good analytical performance with good reproducibility and stability. The linear range of L-cysteine is 0.0001-2 mM and the detection limit is 0.36 μM and 0.17 μM for cvp1 and cvp2, respectively.

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Spectrophotometric determination of cysteine

Abstract: In a study (7) of the reaction between nitrilotriacetatoferrate (III) [Fe(III)NTA] and cysteine, a red-colored mixed complex was observed to form immediately on mixing and subsequently decompose. Investigation showed that the kinetics

Cited by 14 publications

References 8 publications

Colorimetric Determination of Acetylcysteine, Penicillamine, and Mercaptopropionylglycine in Pharmaceutical Dosage Forms

Colorimetric Determination of Acetylcysteine, Penicillamine, and Mercaptopropionylglycine in Pharmaceutical Dosage Forms

Promotion of Replication in Lymphoid Cells by Specific Thiols and Disulfides in Vitro

Growth of Li Doped Bismuth Oxide Nanorods and its Electrochemical Performance for the Determination of L-Cysteine

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